Antimicrobial Wound Dressings against Fluorescent and Methicillin-Sensitive Intracellular Pathogenic Bacteria

Abstract

There is limited evidence indicating that drug-eluting dressings are clinically more effective than simple conventional dressings. To shed light on this concern, we have performed evidence-based research to evaluate the antimicrobial action of thymol (THY)-loaded antimicrobial dressings having antibiofilm forming ability, able to eradicate intracellular and extracellular pathogenic bacteria. We have used four different Staphylococcus aureus strains, including the ATCC 25923 strain, the Newman strain (methicillin-sensitive strain, MSSA) expressing the coral green fluorescent protein from the vector pCN47, and two clinical reference strains, Newman-(MSSA) and USA300-(methicillin-resistant strain), as traceable models of pathogenic bacteria commonly infecting skin and soft tissues. Compared to non-loaded dressings, THY-loaded polycaprolactone-based electrospun dressings were also able to eliminate pathogenic bacteria in coculture models based on infected murine macrophages. In addition, by using confocal microscopy and the conventional microdilution plating method, we corroborated the successful ability of THY in preventing also biofilm formation. Herein, we demonstrated that the use of wound dressings loaded with the natural monoterpenoid phenol derivative THY are able to eliminate biofilm formation and intracellular methicillin-sensitive S aureus more efficiently than with their corresponding THY-free counterparts.

Small Molecule-Capped Gold Nanoclusters for Curing Skin Infections

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Synthesis and Characterization of Size- and Charge-Tunable Silver Nanoparticles for Selective Anticancer and Antibacterial Treatment

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“…Extracted from Thymus vulgaris, Thymol (THY) has shown antimicrobial activity against different bacterial strains and inhibition of the formation of biofilms. Sara Garcia‐Salinas et al 112 loaded THY on PCL ENMs to overcome the solubility limitations, and the studies corroborate that with 5 mg PCL‐THY nanofibers the bacterial attachment to the wall was inhibited and 7 mg PCL‐THY nanofibers could eliminate already formed biofilms of S. aureus . The highest dose of 30 mg PCL‐THY nanofibers could inhibit the clinical strains biofilms MSSA Newman and MRSA USA300.…”