Antimicrobial susceptibility of potentially pathogenic halophilic vibrios isolated from seafood

Ottaviani, Donatella; Bacchiocchi, I.; Masini, Laura; Leoni, Francesca; Carraturo, Antonio; Giammarioli, Monica; Sbaraglia, Giovanni

doi:10.1016/s0924-8579(01)00358-2

Cited by 104 publications

(81 citation statements)

References 18 publications

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“…Li et al [21] reported remarkable sensitivity to the aminoglycosides (streptomycin and kanamycin), in agreement with the observation of this study; however, other reports [16,21] suggested otherwise. Vibrio strains in this study were also considerably sensitive to nalidixic acid, ofloxacin, ciprofloxacin, chloramphenicol, and rifampicin (Table 2), contrary to the observation of Ottaviani et al [22], who reported resistance against rifampicin. Marin et al [16] also reported an intermediate/reduced sensitivity to chloramphenicol and ciprofloxacin, and resistance against nalidixic acid, contrary to the observation of this study.…”

Section: Discussioncontrasting

confidence: 90%

Multi-drug resistant Vibrio species isolated from abattoir effluents in Nigeria

Odjadjare

Igbinosa

2017

J Infect Dev Ctries

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Introduction: The antibiograms of Vibrio species isolated from abattoir effluents in the Niger Delta region of Nigeria were investigated with respect to their public health significance. Methodology: Vibrio species were isolated and identified using standard microbiological and molecular techniques, while antibiogram of isolates was tested and interpreted using the disk diffusion method described by the Clinical and Laboratory Standards Institute. Results: Of 150 presumptive isolates, 48 (32%) were confirmed to be Vibrio spp. by PCR; of these, 23 (47.9%) were V. cholerae, 11 (22.9%) were V. fluvialis, 8 (16.7%) were V. vulnificus, and 6 (12.5%) were V. parahaemolyticus. The antibiogram revealed that Vibrio species were generally resistant to ampicillin (60%-67%), trimethoprim (80%-100%), and tetracycline (60%-83%), whereas they were sensitive to ceftriaxone (86%-100%), the aminoglycosides (67%-100%), imipenem (86%-100%), ofloxacin (83%-100%), and chloramphenicol (67%-100%). The isolates exhibited multiple antibiotic resistance (MAR) with an average MAR index of 0.23. Conclusions: This study demonstrated that abattoir effluents are important reservoirs for multidrug-resistant Vibrio species that might be considerable contributors to the recurrent episodes of epidemic cholera and non-Vibrio cholera infections in Nigeria.

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Section: Discussioncontrasting

confidence: 90%

Multi-drug resistant Vibrio species isolated from abattoir effluents in Nigeria

Odjadjare

Igbinosa

2017

J Infect Dev Ctries

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“…The few recent studies that examined the antimicrobial susceptibilities of non-cholerae Vibrio spp. and other aquatic bacteria were all conducted in other countries and included very limited numbers of either V. parahaemolyticus or V. vulnificus isolates (1,20,25,29,30). Therefore, the present study aimed to provide an update on the antimicrobial susceptibilities of these two important vibrios by using isolates from Louisiana-harvested raw oysters.…”

mentioning

confidence: 99%

Antimicrobial Susceptibilities of Vibrio parahaemolyticus and Vibrio vulnificus Isolates from Louisiana Gulf and Retail Raw Oysters

Han

Walker

Janes

et al. 2007

Appl Environ Microbiol

159

102

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The antimicrobial susceptibilities of 168 Vibrio parahaemolyticus and 151 Vibrio vulnificus isolates recovered from 82 Louisiana Gulf and retail oysters in 2005 and 2006 were determined. Overall, the two vibrios remained susceptible to the majority of antimicrobials tested; reduced susceptibility was detected only in V. parahaemolyticus for ampicillin (81%; MIC ≥ 16 μg/ml). Additionally, V. parahaemolyticus displayed significantly higher MICs for cefotaxime, ciprofloxacin, and tetracycline than V. vulnificus.

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“…S8). The gene encoding β-lactamase was also found in many Vibrio species, including non-O1/non-O139 Vibrio cholerae, Vibrio harveyi, and Vibrio alginolyticus (31,32). These results suggest that direct recognition of β-lactam antibiotic by VbrK is a well-conserved mechanism to induce β-lactamase gene expression in Vibrio species.…”

Section: Discussionmentioning

confidence: 85%

Sensor histidine kinase is a β-lactam receptor and induces resistance to β-lactam antibiotics

Wang

Zhang

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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β-Lactams disrupt bacterial cell wall synthesis, and these agents are the most widely used antibiotics. One of the principle mechanisms by which bacteria resist the action of β-lactams is by producing β-lactamases, enzymes that degrade β-lactams. In Gram-negative bacteria, production of β-lactamases is often induced in response to the antibiotic-associated damage to the cell wall. Here, we have identified a previously unidentified mechanism that governs β-lactamase production. In the Gram-negative enteric pathogen Vibrio parahaemolyticus, we found a histidine kinase/response regulator pair (VbrK/VbrR) that controls expression of a β-lactamase. Mutants lacking either VbrK or VbrR do not produce the β-lactamase and are no longer resistant to β-lactam antibiotics. Notably, VbrK autophosphorylation is activated by β-lactam antibiotics, but not by other lactams. However, single amino acid substitutions in the putative periplasmic binding pocket of VbrK leads its phosphorylation in response to both β-lactam and other lactams, suggesting that this kinase is a β-lactam receptor that can directly detect β-lactam antibiotics instead of detecting the damage to cell wall resulting from β-lactams. In strong support of this idea, we found that purified periplasmic sensor domain of VbrK binds penicillin, and that such binding is critical for VbrK autophosphorylation and β-lactamase production. Direct recognition of β-lactam antibiotics by a histidine kinase receptor may represent an evolutionarily favorable mechanism to defend against β-lactam antibiotics.

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Antimicrobial susceptibility of potentially pathogenic halophilic vibrios isolated from seafood

Cited by 104 publications

References 18 publications

Multi-drug resistant Vibrio species isolated from abattoir effluents in Nigeria

Multi-drug resistant Vibrio species isolated from abattoir effluents in Nigeria

Antimicrobial Susceptibilities of Vibrio parahaemolyticus and Vibrio vulnificus Isolates from Louisiana Gulf and Retail Raw Oysters

Sensor histidine kinase is a β-lactam receptor and induces resistance to β-lactam antibiotics

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