Antimicrobial susceptibility of Histophilus somni isolated from clinically affected cattle in Australia

Goldspink, Lauren; Mollinger, Joanne L.; Barnes, Tamsin S.; Groves, Mitchell D.; Mahony, Timothy J.; Gibson, Justine S.

doi:10.1016/j.tvjl.2014.12.008

Cited by 15 publications

(13 citation statements)

References 15 publications

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“…Bovine respiratory disease (BRD) is an infectious disease that causes a significant economic loss for the beef industry, accounting for 70–80% morbidity and 40–50% mortality. BRD is a major cause of lost production within the Australian feedlot industry with an estimated annual cost of around AUD 40 million [ 1 , 2 , 3 ]. BRD is a complex, multifactorial condition that requires the interaction of animals, infectious agents, and environmental factors.…”

Section: Introductionmentioning

confidence: 99%

“…Studies investigating AMR development in BRD pathogens isolated from Australian feedlot cattle are limited, which may in part be due to the low incidence of BRD and the generally good response to treatment observed in individual BRD cases [ 2 , 14 , 18 ]. Nonetheless, surveillance of AMR in BRD bacterial pathogens is essential to develop treatment protocols that maintain antimicrobial effectiveness, facilitate the implementation of antimicrobial stewardship policies, and improve the health and welfare of animals.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

First Emergence of Resistance to Macrolides and Tetracycline Identified in Mannheimia haemolytica and Pasteurella multocida Isolates from Beef Feedlots in Australia

et al. 2021

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Bovine respiratory disease (BRD) causes high morbidity and mortality in beef cattle worldwide. Antimicrobial resistance (AMR) monitoring of BRD pathogens is critical to promote appropriate antimicrobial stewardship in veterinary medicine for optimal treatment and control. Here, the susceptibility of Mannheimia haemolytica and Pasteurella multicoda isolates obtained from BRD clinical cases (deep lung swabs at post-mortem) among feedlots in four Australian states (2014–2019) was determined for 19 antimicrobial agents. The M. haemolytica isolates were pan-susceptible to all tested agents apart from a single macrolide-resistant isolate (1/88; 1.1%) from New South Wales (NSW). Much higher frequencies of P. multocida isolates were resistant to tetracycline (18/140; 12.9%), tilmicosin (19/140; 13.6%), tulathromycin/gamithromycin (17/140; 12.1%), and ampicillin/penicillin (6/140; 4.6%). Five P. multocida isolates (3.6%), all obtained from NSW in 2019, exhibited dual resistance to macrolides and tetracycline, and a further two Queensland isolates from 2019 (1.4%) exhibited a multidrug-resistant phenotype to ampicillin/penicillin, tetracycline, and tilmicosin. Random-amplified polymorphic DNA (RAPD) typing identified a high degree of genetic homogeneity among the M. haemolytica isolates, whereas P. multocida isolates were more heterogeneous. Illumina whole genome sequencing identified the genes msr(E) and mph(E)encoding macrolide resistance, tet(R)-tet(H) or tet(Y) encoding tetracycline resistance, and blaROB-1 encoding ampicillin/penicillin resistance in all isolates exhibiting a corresponding resistant phenotype. The exception was the tilmicosin-resistant, tulathromycin/gamithromycin-susceptible phenotype identified in two Queensland isolates, the genetic basis of which could not be determined. These results confirm the first emergence of AMR in M. haemolytica and P. multocida from BRD cases in Australia, which should be closely monitored.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

First Emergence of Resistance to Macrolides and Tetracycline Identified in Mannheimia haemolytica and Pasteurella multocida Isolates from Beef Feedlots in Australia

et al. 2021

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“…BRD control is thus a major target of antimicrobial usage [90, 91], and possibly an important source of AMR pathogens. BRD involves a complex of etiological agents including Mannheimia haemolytica, the predominant agent [92], Pasteurella multocida , and Histophilus somni [92, 93]. H. somni occurs sporadically, and can cause fatal septicemia in cattle.…”

Section: Antimicrobial Resistance In Bovine Pathogensmentioning

confidence: 99%

Antimicrobial usage and resistance in beef production

Cameron

McAllister

2016

J Animal Sci Biotechnol

140

122

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Antimicrobials are critical to contemporary high-intensity beef production. Many different antimicrobials are approved for beef cattle, and are used judiciously for animal welfare, and controversially, to promote growth and feed efficiency. Antimicrobial administration provides a powerful selective pressure that acts on the microbial community, selecting for resistance gene determinants and antimicrobial-resistant bacteria resident in the bovine flora. The bovine microbiota includes many harmless bacteria, but also opportunistic pathogens that may acquire and propagate resistance genes within the microbial community via horizontal gene transfer. Antimicrobial-resistant bovine pathogens can also complicate the prevention and treatment of infectious diseases in beef feedlots, threatening the efficiency of the beef production system. Likewise, the transmission of antimicrobial resistance genes to bovine-associated human pathogens is a potential public health concern. This review outlines current antimicrobial use practices pertaining to beef production, and explores the frequency of antimicrobial resistance in major bovine pathogens. The effect of antimicrobials on the composition of the bovine microbiota is examined, as are the effects on the beef production resistome. Antimicrobial resistance is further explored within the context of the wider beef production continuum, with emphasis on antimicrobial resistance genes in the food chain, and risk to the human population.

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“…Culture has the advantage of providing the laboratory isolates for use in antimicrobial susceptibility assays, MALDI-TOF mass spectroscopic characterization, other comparative analyses, and epidemiological studies (Tegtmeier et al 2000;Portis et al 2012;Kuhnert et al 2012;Frey and Kuhnert 2015). Antimicrobial susceptibility assays for H. somni include disk diffusion (Kirby-Bauer, Edisk) and broth microdilution (D'Amours et al 2011;Goldspink et al 2015). H. somni is susceptible to multiple antimicrobial classes in vitro.…”

Section: Laboratory Diagnosis and Antimicrobial Resistance Testingmentioning

confidence: 99%

Histophilosis as a Natural Disease

O’Toole

Sondgeroth

2015

Current Topics in Microbiology and Immunology

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Histophilus somni is responsible for sporadic disease worldwide in cattle and, to a lesser extent, in small ruminants, bighorn sheep (Ovis canadensis), and North American bison (Bison bison). The importance of H. somni diseases can be attributed to improved clinical and laboratory recognition, combined with the growth in intensive management practices for cattle. Although outbreaks of bovine histophilosis can occur year-round, in northern and southern hemispheres, it is most frequent in late fall and early winter. Weather, stress, dietary changes, and comingling of cattle are likely to be major triggers for outbreaks. The most frequent clinical expressions of histophilosis include undifferentiated fever, fibrinosuppurative pneumonia, encephalitis-leptomeningitis, necrotizing myocarditis, and diffuse pleuritis. Neurological disease occurs either as thrombotic meningoencephalitis (TME) or as suppurative meningitis with ventriculitis. Acute myocarditis is characteristically necrotizing and generally involves one or both papillary muscles in the left ventricular myocardium. Biofilm-like aggregates of bacteria occur in capillaries and veins in myocardium, in the central nervous system, and on endocardial surfaces. H. somni is a component of bovine respiratory disease (BRD) complex. In our experience, it is most commonly diagnosed in subacute-to-chronic polymicrobial pulmonary infections in combination with Mannheimia haemolytica, Trueperella pyogenes, Pasteurella multocida, or Mycoplasma bovis. Other, less common forms of H. somni disease present as polyarthritis/tenosynovitis, abortion with placentitis and fetal septicemia, epididymitis-orchitis, and ocular infections. It is likely that H. somni is under-recognized clinically and diagnostically. Most state and provincial laboratories in North America rely on bacterial isolation to confirm infection. The use of more sensitive detection methods on field cases of histophilosis will help resolve the pathogenesis of H. somni in natural outbreaks, and whether the disease is as common elsewhere as it is in Canada.

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Antimicrobial susceptibility of Histophilus somni isolated from clinically affected cattle in Australia

Cited by 15 publications

References 15 publications

First Emergence of Resistance to Macrolides and Tetracycline Identified in Mannheimia haemolytica and Pasteurella multocida Isolates from Beef Feedlots in Australia

First Emergence of Resistance to Macrolides and Tetracycline Identified in Mannheimia haemolytica and Pasteurella multocida Isolates from Beef Feedlots in Australia

Antimicrobial usage and resistance in beef production

Histophilosis as a Natural Disease

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