Antimicrobial potential of mentha spp. essential oils as raw and loaded solid lipid nanoparticles against dental caries

Mostafa, Dalia A.; Bayoumi, Faten S.; Taher, Heba M.; Abdelmonem, Basma H.; Eissa, Tarek F.

doi:10.5958/0974-360x.2020.00781.7

Cited by 6 publications

(6 citation statements)

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“…In particular, CT-NLC ( Figure 6 a,b) showed a very irregular arrangement of nanoparticles, with a central oily core and a large shell. N-NLC ( Figure 6 c,d) and M-NLC ( Figure S4 ) showed the presence of a faint central oily core surrounded by the solid lipid and surfactants layers, in agreement with previous studies [ 63 , 64 ]. On the other side, L-NLC ( Figure 6 f) and R-NLC ( Figure S5 ) showed a structure characterized by the presence of many oily droplets dispersed in the solid lipid matrix, in agreement with previous findings [ 65 ].…”

Section: Resultssupporting

confidence: 92%

“…No cytotoxic effects were observed when murine fibroblasts were treated with N-NLC, while the highest concentration (500 µg/mL) significantly reduced human fibroblast viability by a hefty 60%. This result is supported by previous studies showing Neem cytotoxic effects on different cell lines (embryonic 3T3 fibroblasts, HeLa tumor cells, HaCat keratinocytes, and V79-4 pulmonary fibroblasts) when used at low concentration and in combination with oleic acid [63]. Furthermore, Neem oil was found to induce cell death in a time-dependent manner on HCT116 cells, whose effect was attributed to the cell cycle arrest and apoptosis due to Neem limonoids, even if the molecular mechanism of Neem limonoid-induced cell death has not been described [68].…”

Section: In Vitro Cytocompatibilitysupporting

confidence: 88%

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Essential Oil-Loaded NLC for Potential Intranasal Administration

et al. 2021

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Complementary and alternative medicines represent an interesting field of research on which worldwide academics are focusing many efforts. In particular, the possibility to exploit pharmaceutical technology strategies, such as the nanoencapsulation, for the delivery of essential oils is emerging as a promising strategy not only in Italy but also all over the world. The aim of this work was the development of nanostructured lipid carriers (NLC) for the delivery of essential oils (Lavandula, Mentha, and Rosmarinus) by intranasal administration, an interesting topic in which Italian contributions have recently increased. Essential oil-loaded NLC, projected as a possible add-on strategy in the treatment of neurodegenerative diseases, were characterized in comparison to control formulations prepared with Tegosoft CT and Neem oil. Homogeneous (polydispersity index, PDI < 0.2) nanoparticles with a small size (<200 nm) and good stability were obtained. Morphological and physical-chemical studies showed the formation of different structures depending on the nature of the liquid oil component. In particular, NLC prepared with Lavandula or Rosmarinus showed the formation of a more ordered structure with higher cytocompatibility on two cell lines, murine and human fibroblasts. Taken together, our preliminary results show that optimized positively charged NLC containing Lavandula or Rosmarinus can be proposed as a potential add-on strategy in the treatment of neurodegenerative diseases through intranasal administration, due to the well-known beneficial effects of essential oils and the mucoadhesive properties of NLC.

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Section: Resultssupporting

confidence: 92%

Section: In Vitro Cytocompatibilitysupporting

confidence: 88%

Essential Oil-Loaded NLC for Potential Intranasal Administration

et al. 2021

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“…Solid lipid nanoparticles of CuSQ were prepared by w/o/w type double emulsification method as shown in Table 6. The preparation method was previously described elsewhere [46]. The surface morphology of CuSQ SLNs was observed by scanning electron microscope (SEM).…”

Section: Synthesis Of Cusq Solid Lipid Nanoparticles (Sln)mentioning

confidence: 99%

“…PDI values bigger than 0.7 indicate that the sample has a very broad particle size distribution. Zeta potential (surface charge) was determined by measuring the electrophoretic mobility of nanoparticles using Malvern Mastersizer [46].…”

Section: Particle Size Analysis Polydispersity Index and Zeta Potentialmentioning

confidence: 99%

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Enhanced In Vivo Wound Healing Efficacy of a Novel Hydrogel Loaded with Copper (II) Schiff Base Quinoline Complex (CuSQ) Solid Lipid Nanoparticles

et al. 2022

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Wound dressings created using nanotechnology are known as suitable substrates to speed up the healing of both acute and chronic wounds. Therapeutic substances can be delivered using these materials. In this study, a hydrogel loaded with Cu (II) Schiff base 8-hydroxy quinoline complex (CuSQ) solid lipid nanoparticles (SLN) was formulated to investigate its wound healing potential in an excision wound healing model in rats. The CuSQ SLN were spherical shaped with sizes ranging from 111 to 202 nm and a polydispersity index (PDI) ranging from 0.43 to 0.76, encapsulation efficiency (EE) % between 85 and 88, and zeta potential (ZP) of −11.8 to −40 mV. The formulated hydrogel showed good homogeneity, good stability, and a pH of 6.4 which indicates no skin irritation and had no cytotoxicity on the human skin fibroblast (HSF) cell line. In the in vivo study, animals were placed in five groups: control, standard, plain hydrogel, low dose, and high dose of CuSQ hydrogel. Both doses of CuSQ showed significantly faster healing rates compared to standard and control rats. In addition, the histopathology study showed more collagen, improved angiogenesis, and intact re-epithelization with less inflammation. A significant increase in transforming growth factor-beta1 (TGF-β1) level and increased immune expression of vascular endothelial growth factor (VEGF) by CuSQ treatment validates its role in collagen synthesis, proliferation of fibroblasts and enhancement of angiogenesis. Matrix metalloproteinase-9 (MMP-9) was found to be significantly reduced after CuSQ treatment. Immunohistochemistry of tumor necrosis factor alpha (TNF-α) revealed a marked decrease in inflammation. Thus, we concluded that CuSQ would be a beneficial drug for cutaneous wound healing since it effectively accelerated wound healing through regulation of various cytokines and growth factors.

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