Antimicrobial high molecular weight pectin polysaccharides production from diverse citrus peels using a novel PL10 family pectate lyase

A CRISPR/Cas9-based visual toolkit enabling multiplex integration at specific genomic loci in Aspergillus niger

“…Xylanase activity was quantified in units (U), representing the amount of enzyme required to produce 1 μmol of reducing sugar under the specified assay conditions (50 °C, pH 5.5). Similarly, the activity of pelA was determined using the DNS method [ 23 ]. Briefly, 50 μL of appropriately diluted enzyme solution was added to 450 μL of 0.1% polygalacturonic acid substrate (Tris-HCl, pH 7.0).…”

Biochemical characterization of extended-spectrum β-lactamases fromAkkermansiagenus

“…To investigate the effect of temperature on enzyme activity, the purified β-lactamase was added to ampicillin in 20 mM Tris-HCl buffer (pH 7.0), and the reaction was carried out at 10, 20, 30, 40, 50, 60, 70, 80, 90, and 100°C for 10 min, respectively (24). Subsequently, the enzyme activity was measured according to the method outlined in Section 2.4.…”

“…To test pH stability, the purified β-lactamase was added to different pH buffers and incubated at 4°C for 1 h. Subsequently, the enzyme activity was evaluated. (24).…”