Antimicrobial efficacy of ozonated water, gaseous ozone, sodium hypochlorite and chlorhexidine in infected human root canals

Estrela, Carlos; Decúrcio, Daniel de Almeida; Hollanda, Augusto César Braz; Silva, J. A.

doi:10.1111/j.1365-2591.2006.01185.x

Cited by 160 publications

(180 citation statements)

References 47 publications

Supporting

Mentioning

131

Contrasting

Unclassified

Order By: Relevance

“…This is in accordance with the results obtained by other authors [14,[19][20][21]. However, it is in disagreement with Estrela et al, [22]. It was found to be more effective than CH, which is in agreement with results obtained by Ballal et al, and Gomes et al, [21,23].…”

Section: Resultssupporting

confidence: 89%

See 1 more Smart Citation

Study Comparing the Effectiveness of Chlorhexidine, Calcium Hydroxide and Linezolid Based Medicaments Against Enterococcus Faecalis

Pavaskar¹

2014

JCDR

View full text Add to dashboard Cite

Section: Resultssupporting

confidence: 89%

“…EF can survive inside dentin tubules for atleast 10 days without nutrient supply [16,30]. It may also survive in smear layer and debris, and be extremely difficult to eliminate during instrumentation and irrigation [22]. Moreover, serine protease and ACE aid in the adhesion of EF with dentin [31].…”

Section: Resultsmentioning

confidence: 99%

Study Comparing the Effectiveness of Chlorhexidine, Calcium Hydroxide and Linezolid Based Medicaments Against Enterococcus Faecalis

Pavaskar¹

2014

JCDR

View full text Add to dashboard Cite

“…The ability to invade the dentinal tubules, be resistant to antimicrobial agents and interfere with host defenses reveals its pathogenic role [6][7][8]. The use of antibacterial strategies that can promote the disruption of the biofilm is essential to the success of the endodontic therapy [3,5].…”

Section: Introductionmentioning

confidence: 99%

Antibacterial action of red and green propolis extract in infected root canal

Endo

Estrela

Alencar

et al. 2017

Rev. Odonto Ciênc. (Online)

View full text Add to dashboard Cite

OBJECTIVE: This study investigated the antibacterial action of 30% red propolis, 40% green propolis and 2.5% sodium hypochlorite with irrigation protocols in infected root canals. METHODS: During 60 days, twenty-four root canals were inoculated with E. faecalis. In all experimental groups were performed root canal preparation and two irrigation protocols -passive ultrasonic irrigation and conventional irrigation. In the groups 1, 3, 5 and 7 it were made root canal preparation associated to conventional irrigation with 30% propolis, 40% propolis, 2.5% sodium hypochlorite and distilled water, respectively. In groups 2, 4, 6 and 8 it was made root canal preparation associated with passive ultrasonic irrigation with the solutions described above. The groups 9 and 10 were the controls (negative and positive). Samples of the root canals were collected and immersed in 7 mL of BHI, for 48 hours, at 37ºC. Bacterial growth was analyzed by turbidity of the culture medium. RESULTS: Antibacterial action was observed of 30% red propolis and 2.5% sodium hypochlorite when used passive ultrasonic irrigation and conventional irrigation after 20 minutes, but in 72 hours it was not effective. CONCLUSION: The irrigating agents and protocols were not effective to eliminate the microorganisms of the infected root canals. Clinical Significance: Propolis presents potential for clinical application due to its antimicrobial, anti-inflammatory, antioxidant and low toxicity properties. Key words: Endodontic; Propolis; Sodium hypochlorite Ação antimicrobiana do extrato de própolis vermelho e verde em canais radiculares infectados RESUMO OBJETIVO: Investigar a ação antibacteriana do própolis vermelhos 30%, do própolis verde 40% e do hipoclorito de sódio 2,5% com protocolos de irrigação em canais radiculares infectados. MÉTODOS: Durante 60 dias, vinte e quatro canais radiculares foram inoculados com E. faecalis. Em todos os grupos experimentais foi realizado o preparo do canal radicular e dois protocolos de irrigação -irrigação ultra-sônica passiva e irrigação convencional. Nos grupos 1, 3, 5 e 7 foi feito o preparo do canal radicular associado à irrigação convencional com própolis 30%, própolis 40%, hipoclorito de sódio 2,5% e água destilada, respectivamente. Nos grupos 2, 4, 6 e 8 foi feito o preparo do canal radicular associado à irrigação ultra-sônica passiva com as soluções descritas acima. Os grupos 9 e 10 foram os controles (negativos e positivos). Amostras dos canais radiculares foram coletadas e imersas em 7 mL de BHI, durante 48 horas, a 37ºC. O crescimento bacteriano foi analisado por turvação do meio de cultura. RESULTADOS: Ação antibacteriana foi observada com própolis vermelho 30% e hipoclorito de sódio 2,5% quando utilizado irrigação ultra-sônica passiva e irrigação convencional após 20 minutos, porém, após 72 horas as substâncias não foram efetivas. CONCLUSÃO: Os agentes de irrigação e os protocolos não foram efetivos para eliminar os microorganismos dos canais radiculares infectados.

show abstract

“…It also covalently binds to metal ions and sequesters calcium ions present in hydroxyapatite dentin. 18 NaOCl is an auxiliary irrigant that is used during root canal instrumentation to promote debridement, lubrication, disinfection, tissue dissolution, collagen layer removal and dentin dehydration ,14, 15,16 and that also causes protein denaturation. 17 EDTA condit ion i ng of dent i ne promotes adhesion, migration and differentiation of the pulp stem cells from permanent teeth.…”

Section: Introductionmentioning

confidence: 99%

“…4 Two irrigants currently used during cleaning and shaping of the root canal are ethylenediaminetetraacetic acid (EDTA) 13 and sodium hypochlorite (NaOCl). 14, 15,16,17 EDTA is a final irrigant that causes dentin demineralization and provides excellent cleaning of the canal walls. 13 It acts on the inorganic components of the smear layer, leading to decalcification of the peri-and intertubular dentin.…”

Section: Introductionmentioning

confidence: 99%

Effect of EDTA on TGF-β1 released from the dentin matrix and its influence on dental pulp stem cell migration

et al. 2016

View full text Add to dashboard Cite

Bioactive molecules stored in dentin, such as transforming growth factor beta1 (TGF-b1), may be involved in the signaling events related to dental tissue repair. The authors conducted an in vitro evaluation of the amount of TGF-b1 released from dentin slices after treatment with 10% ethylenediaminetetraacetic acid (EDTA), 2.5% sodium hypochlorite (NaOCl) or phosphate-buffered saline (PBS), and the effect of this growth factor on stem cell migration from human exfoliated deciduous teeth (SHED). Sixty 1-mm-thick tooth slices were prepared with or without the predentin layer, and treated with either 10% EDTA for 1 minute, 2.5% NaOCl for 5 days or kept in PBS. Tooth slice conditioned media were prepared and used for TGF-b1 ELISA and migration assays. Culture medium with different concentrations of recombinant human TGF-b1 (0.5, 1.0, 5.0 or 10.0 ng/mL) was also tested by migration assay. The data were evaluated by ANOVA and Tukey's test. Optical density values corresponding to media conditioned by tooth slices either containing or not containing the predentin layer and treated with 10% EDTA were statistically greater than the other groups and close to 1 ng/mL. Increased rates of migration toward media conditioned by tooth slices containing the predentin layer and treated with PBS, 10% EDTA or 2.5% NaOCl were observed. Recombinant human TGF-b1 also stimulated migration of SHED, irrespective of the concentration used. EDTA may be considered an effective extractant of TGF-b1 from the dentin matrix. However, it does not impact SHED migration, suggesting that other components may account for the cell migration.

show abstract

Antimicrobial efficacy of ozonated water, gaseous ozone, sodium hypochlorite and chlorhexidine in infected human root canals

Abstract: The irrigation of infected human root canals with ozonated water, 2.5% NaOCl, 2% chlorhexidine and the application of gaseous ozone for 20 min was not sufficient to inactivate E. faecalis.

Cited by 160 publications

References 47 publications

Study Comparing the Effectiveness of Chlorhexidine, Calcium Hydroxide and Linezolid Based Medicaments Against Enterococcus Faecalis

Study Comparing the Effectiveness of Chlorhexidine, Calcium Hydroxide and Linezolid Based Medicaments Against Enterococcus Faecalis

Antibacterial action of red and green propolis extract in infected root canal

Effect of EDTA on TGF-β1 released from the dentin matrix and its influence on dental pulp stem cell migration

Contact Info

Product

Resources

About