Antimicrobial, antioxidant and cytotoxic activities of propolis from Melipona orbignyi (Hymenoptera, Apidae)

Campos, Jaqueline Ferreira; Santos, Uilson Pereira dos; Macorini, Luís Fernando Benitez; Melo, Adriana Mary Mestriner Felipe de; Balestieri, José Benedito Perrella; Paredes‐Gamero, Edgar Julian; Cardoso, Cláudia Andréa Lima; Souza, Kely de Picoli; Santos, Edson Lucas dos

doi:10.1016/j.fct.2014.01.008

Cited by 125 publications

(116 citation statements)

References 33 publications

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“…Nevertheless, the antioxidant profile of M. mondury geopropolis was higher compared with that of M. subnitida geopropolis, which is known as Jandaíra in northeastern Brazil (Souza et al 2013). Previously, it has been suggested that reducing compounds such as phenolics are responsible for the antioxidant activity of geopropolis (Souza et al 2013, Campos et al 2014, Dutra et al 2014, which may explain why the BFGP had the highest antioxidant activity in all the assays. Infectious diseases are important causes of morbidity and mortality among humans, especially in developing and poor countries.…”

Section: Resultsmentioning

confidence: 99%

“…Geopropolis has also been proposed as an alternative for prevention and/or treatment of infectious diseases because it has broad antimicrobial activities. In fact, several types of geopropolis have antibacterial properties (Liberio et al 2011, Campos et al 2014.…”

Section: Resultsmentioning

confidence: 99%

“…The biological activities and chemical compositions of geopropolis have been reported for many different stingless bee species (Liberio et al 2011, Campos et al 2014. However, there have been no previous pharmacological and chemical analyses of geopropolis from M. mondury.…”

Section: Introductionmentioning

confidence: 99%

“…Studies of different types of geopropolis have demonstrated their significant antinociceptive , anti-inflammatory , Campos et al 2015, antioxidant , Campos et al 2014, immunomodulatory (Liberio et al 2011), antibacterial (Liberio et al 2011, Campos et al 2014, and antiproliferative , Campos et al 2014 activities. Their chemical compositions are extremely complex and their diversity depends on the flora visited and the season (Marcucci 1995, Bankova et al 2000, Sforcin and Bankova 2011.…”

Section: Introductionmentioning

confidence: 99%

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Melipona mondury produces a geopropolis with antioxidant, antibacterial and antiproliferative activities

Santos

Queiroz

Sawaya

et al. 2017

An. Acad. Bras. Ciênc.

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Geopropolis is a special type of propolis produced by stingless bees. Several pharmacological properties have been described for different types of geopropolis, but there have been no previous studies of the geopropolis from Melipona mondury. In this study, we investigated the antioxidant, antibacterial, and antiproliferative activities of M. mondury geopropolis, and determined its chemical profile. The antioxidant activity was determined using in vitro ABTS ·+ , · DPPH, and β-carotene/linoleic acid co-oxidation methods. The antibacterial activity was determined using a microdilution method with Pseudomonas aeruginosa, Staphylococcus aureus, and methicillin-resistant S. aureus. The antiproliferative effect was determined in tumor cell lines using the Alamar Blue assay. The chemical profile was obtained using UHPLC-MS and UHPLC-MS/MS. The butanolic fraction had the highest concentration of phenolic compounds and more potent antioxidant properties in all assays. This fraction also had bacteriostatic and bactericidal effects against all bacterial strains at low concentrations, especially S. aureus. The hexane fraction had the highest antiproliferative potential, with IC 50 values ranging from 24.2 to 46.6 µg/mL in HL-60 (human promyelocytic leukemia cell) and K562 (human chronic myelocytic leukemia cell), respectively. Preliminary chemical analysis indicates the presence of terpenes and gallic acid in the geopropolis. Our results indicate the therapeutic potential of geopropolis from M. mondury against inflammatory, oxidative, infectious, and neoplastic diseases.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Melipona mondury produces a geopropolis with antioxidant, antibacterial and antiproliferative activities

Santos

Queiroz

Sawaya

et al. 2017

An. Acad. Bras. Ciênc.

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“…While, Choudhari et al (2013) found that IC 50 for ethanolic extract of propolis (EEP) of four cancer cell lines: human colon adenocarcinoma (HT-29), human epithelial colorectal adenocarcinoma (Caco-2), and murine melanoma cell lines (B16F1), MCF-7 to be 250 µg/mL. In another study, Campos et al (2014) reported that EEP promoted cytotoxic activity and primarily necrotic death in K562 erythroleukemia cells (Barzin et al, 2011;Campos et al, 2014). On the other hand, fewer studies reported cytotoxicity of bee pollen (Barzin et al, 2011).…”

Section: Resultsmentioning

confidence: 99%

A Comparative Study on Propolis and Pollen Extracts: Chemical Profile Analysis, Antioxidant and Anticancer Activity

Shady¹,

Mohamed²,

Sayed-Ahmed³

et al. 2016

Int.J.Curr.Microbiol.App.Sci

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Structure Determination of Monomeric Phloroglucinol Derivatives with a Cinnamoyl Group Isolated from Propolis of the Stingless Bee, Tetragonula carbonaria

et al. 2016

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Tetragocarbones A and B were isolated from the propolis of an Australian stingless bee, Tetragonula carbonaria. These largely planar structures were proposed as a result of NMR analysis as well as comparison of their NMR data with those of related monomeric phloroglucinol derivatives. To elucidate relative and absolute stereochemistry of tetragocarbone A, the synthesis of the proposed structure of tetragocarbone A was performed. As a result, the synthetic compound bearing stereogenic centers 1R*,3R*,6S* was identical with naturally occurring tetragocarbone A. The absolute stereochemistry of tetragocarbone A was established to be 1R,3R,6S by chiral HPLC separation and X-ray analysis. Comparison of the NMR data of tetragocarbone A with that of tetragocarbone B indicated that tetragocarbone B would be a natural congener of tetragocarbone A.Stingless bees are a large group of social honey bees and live in tropical and subtropical areas.[1] Stingless bees produce propolis as their hive material. The propolis of stingless bees has been use in traditional medicines and health supplements worldwide. Because of their pharmacological utility, the propolis of stingless bees has attracted much attention as a rich source of biologically active natural products. [2,3] Many efforts have been devoted to analyzing and identifying biologically active natural products in the propolis. [2,3] However, to the best of our knowledge, novel natural products have not been isolated from the propolis of stingless bees. Herein, we report the isolation and structure determination of monomeric phloroglucinol derivatives [4] tetragocarbone A (1 a) and B (1 b, Figure 1) isolated from the propolis of the Australian social stingless bee, Tetragonula carbonaria (= Trigona carbonaria). Tetragocarbone A and B are the first examples of tetramethylated monomeric phloroglucinol derivatives bearing a cinnamoyl group. The relative and absolute stereochemistry of 1 a was unambiguously determined by its total synthesis and X-ray crystal analysis. Comparison of the NMR data of tetragocarbone B (1 b) with that of 1 a provides a proposed structure of 1 b that would be a natural congener of 1 a. Hexane extracts of the propolis of T. carbonaria were purified by repeated normal phase silica gel chromatography, normal phase HPLC, and chiral normal phase HPLC to give 1 a and 1 b (Scheme S1 in the supporting information). Tetragocarbone A (1 a) and B (1 b) were obtained as yellow oil. These optical rotations were found to be levorotatory (1 a: [a] = À13.6 (c = 0.28, CHCl 3 ), 1 b: [a] = À17.1 (c = 0.14, CHCl 3 )). The molecular formulas of 1 a and 1 b were putatively assigned as C 26 The planar structures of 1 a and 1 b were proposed as a result of NMR analysis and comparison of their NMR data with those of monomeric phloroglucinols 2 a, 2 b, and 3 [a] Dr.

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Antimicrobial, antioxidant and cytotoxic activities of propolis from Melipona orbignyi (Hymenoptera, Apidae)

Cited by 125 publications

References 33 publications

Melipona mondury produces a geopropolis with antioxidant, antibacterial and antiproliferative activities

Melipona mondury produces a geopropolis with antioxidant, antibacterial and antiproliferative activities

A Comparative Study on Propolis and Pollen Extracts: Chemical Profile Analysis, Antioxidant and Anticancer Activity

Structure Determination of Monomeric Phloroglucinol Derivatives with a Cinnamoyl Group Isolated from Propolis of the Stingless Bee, Tetragonula carbonaria

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