Antimicrobial activity, phenolic profile and role in the inflammation of propolis

Abstract

a b s t r a c tNowadays a great amount of information regarding chemical and biological aspects of bee products is available in the literature, but few data on its therapeutic uses are found. The aim of this study was to evaluate the phenolic profile, the in vitro antimicrobial activity and effect in the hyaluronidase enzyme (widely related with the inflammation process) of propolis harvested in Portugal. The efficacy of three extracts (hydro-alcoholic, methanolic and aqueous) was also compared. It was chosen the hydroalcoholic extract, because this was the most effective for extracting phenolic compounds. The antimicrobial activity was accessed in Gram-positive and Gram-negative bacteria and yeasts, isolated from different biological fluids and the results were then compared with the obtained for reference microorganisms. The propolis from Bragança was the one that possessed the highest polyphenols' content. The sample from Beja showed the less significant inhibition of the hyaluronidase enzyme. Concerning the antimicrobial activity, Candida albicans was the most resistant and Staphylococcus aureus the most sensitive. The reference microorganisms were more sensitive than the ones isolated from biological fluids.

Propolis and its constituent caffeic acid suppress LPS-stimulated pro-inflammatory response by blocking NF-κB and MAPK activation in macrophages

“…This product has attracted researchers' interest in the last decade because of its numerous biological and pharmacological properties, such as antimicrobial (Sforcin et al, 2000;Freitas et al, 2006;Búfalo et al, 2009), antitumoral (Bazo et al, 2002), immunomodulatory (Sforcin, 2007), anti-inflammatory (Silva et al, 2012), antioxidant (Kumazawa et al, 2010), among others.…”

Preliminary Studies of the Immunomodulator Effect of the Propolis Trigona spp. Extract in a Mouse Model

“…[2,3,4,5,6]. This product has attracted the interest of researchers in recent decades because of the properties of biological and pharmacological, among others such as antimicrobial [7,8,9], antitumor [10], immunomodulator [11], anti-inflammatory [12], an antioxidant [13].…”

Development and characterization of orally-disintegrating films for propolis delivery

“…Among propolis characteristics, antimicrobial, antifungal, antioxidant, anti-inflammatory properties may be emphasized (KOÇ et al, 2011;SILVA et al, 2012) making it a potential source of active components.…”