Antimicrobial activity of Prunella Vulgaris extracts against multi-drug resistant Escherichia Coli from patients of urinary tract infection

Komal, Sumra; Kazmi, Syed Asif Jahanzeb; Khan, Junaid; Gilani, Mashkoor Mohsin

doi:10.12669/pjms.343.14982

Cited by 20 publications

(22 citation statements)

References 17 publications

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“…Multi-drug resistant bacteria are serious threat in clinical health settings and very challenging to treat infectious disease 8. From current study it was found that 84% of clinical isolates have multi-drug resistance pattern.…”

Section: Discussionmentioning

confidence: 71%

Antibacterial effect of copper sulfate against multi-drug resistant nosocomial pathogens isolated from clinical samples

Benhalima

Amri²,

Bensouilah³

et al. 2019

Pak J Med Sci

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Background and Objective: With the emergence of antibiotic resistance and the hospital acquired infection, the interest for antimicrobial agents has recently increased again in public health. Copper is recommended as a supplementary method of increasing biological safety in the hospital environment. The objective of this study was to determine the antibacterial activity of copper sulfate salts on strains of bacterial pathogens isolated from different clinical pictures in different health establishment in Algeria. Methods: A total of 25 different bacterial isolates (16 Enterobacteriaceae, 5 Staphylococci, and 4 Pseudomonas) were tested for susceptibility to copper sulfate using minimum inhibitory concentration (MIC-Cu) and minimum bactericidal concentrations (MBC-Cu) determinations. All isolates were also tested for susceptibility to six antibiotics. Results: Antibiotic susceptibility studies revealed that 100% of isolates were resistant to one or more antibiotics. Fifty two percent of isolates were very susceptible to copper sulfate, with MICs ranging from 100 to 200 µg/ml. MBC-Cu = 1600 μg/ml showed the best bactericidal effect against the great majority of studied bacteria (52%). A good bactericidal activities of copper sulfate were recorded against Proteus vulgaris and Staphylococcus aureus (MBC/MIC=1). The Gram-negative bacteria isolates which were copper resistant also showed a high resistance to chloramphenicol (r=0.78) and Trimethoprime (r=0.61). Furthermore, the strains that were no-susceptible to three different antimicrobial classes (Escherichia coli, Staphylococcus saprophyticus) were not resistant to copper sulfate. Conclusion: Copper sulfate salts has significant antibacterial activity against multi-drug resistant nosocomial pathogens. doi: https://doi.org/10.12669/pjms.35.5.336 How to cite this:Benhalima L, Amri S, Bensouilah M, Ouzrout R. Antibacterial effect of copper sulfate against multi-drug resistant nosocomial pathogens isolated from clinical samples. Pak J Med Sci. 2019;35(5):---------. doi: https://doi.org/10.12669/pjms.35.5.336 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Section: Discussionmentioning

confidence: 71%

Antibacterial effect of copper sulfate against multi-drug resistant nosocomial pathogens isolated from clinical samples

Benhalima

Amri²,

Bensouilah³

et al. 2019

Pak J Med Sci

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“…Aproximadamente 80% da administração de antimicrobianos são desnecessárias. O uso incorreto dos antimicrobianos desempenha um papel significativo no desenvolvimento de resistência entre os microrganismos, produzindo efeitos adversos significativos sobre a saúde humana (25).…”

Section: Resultsunclassified

“…A partir de um estudo realizado com Prunella vulgaris L., foi observado que essa espécie, quando utilizada com antibióticos convencionais, no tratamento de ITU, apresentou melhores resultados, aumentando a sua eficácia, reduzindo a toxicidade e proporcionando uma atividade antimicrobiana de largo espectro (25). 10.14450/2318-9312.v33.e1.a2021.pp18-30…”

Section: Resultsunclassified

Infecções Do Trato Urinário: Uma Revisão Sobre as Evidências Científicas Das Principais Plantas Medicinais Utilizadas Na Prática Clínica

Lopes¹,

Cechinel-Zanchett²

2021

Infarma

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Infecções do trato urinário (ITU) caracterizam-se pela resposta do organismo diante uma invasão e multiplicação bacteriana. O tratamento através de terapias alternativas, entre elas plantas medicinais, tem se mostrado eficaz na prevenção de patologias. Dessa forma, o presente estudo objetivou realizar uma revisão bibliográfica sobre as principais plantas medicinais utilizadas para o tratamento de ITU. A busca foi realizada nas bases de dados PubMed, Scielo, Medline, Science Direct e Google acadêmico, nos idiomas português, inglês e espanhol e em documentos oficiais dos últimos 12 anos. Sugere-se que os constituintes fitoquímicos presentes em algumas espécies, agem modulando o estresse oxidativo, impedem a fixação e proliferação de microrganismos. Existem muitos estudos sobre o potencial das plantas medicinais nas ITU, porém ainda são necessárias mais investigações visando o estabelecimento de doses, forma e tempo de uso, além de seus respectivos mecanismos de ação.

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“…Studies on the action of aqueous, alcoholic and hydroalcoholic extract have highlighted the antimicrobial, antifungal, and antiviral properties. The aqueous and hydroalcoholic extract of the species Prunella vulgaris L. revealed the anti-bacterial effect on Escherichia coli, Klebsiella pneumoniae, Proteus vulgaris, and on different Staphylococcus species (7)(8)(9). The methanolic extract of Prunella vulgaris L. (root) has demonstrated in vitro antiproliferative activity on different breast cancer cell lines: MCF-7, MDA-MB-231BO.…”

Section: Introductionmentioning

confidence: 99%

Antibacterial and antitumor activity of the species Prunella vulgaris L.

Groşan

Vari

Ștefănescu

et al. 2020

Revista Romana De Medicina De Laborator

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Background: Prunella vulgaris L., known as self-healing herb, is a widely spread species in the spontaneous flora with beneficial effects on human health, a fact proven in particular by Asian researchers. The aim of this study was to evaluate the antitumor activity and the antibacterial effect on different bacterial strains, including multidrug-resistant ones, depending on the type of solvent used (aqueous, hydroalcoholic), the plant product taken into consideration (spike inflorescence, leaf), its quantity and the concentration of active principles.Material and method: For screening of antimicrobial susceptibility, both minimum inhibitory concentration and minimum bactericidal concentration were determined on Escherichia coli, Klebsiella pneumoniae, Providencia stuartii, Pseudomonas aeruginosai, and methicillin-resistant Staphylococcus aureus species, including reference strains and hospital strains. Leaves and flower extracts (aqueous and 70% methanolic) were first assessed, and the one with the best antibacterial potential was further tested as a concentrated extract. The antitumor activity was determined on MDA-MB-231 breast adenocarcinoma cells and on a non-tumor cell line, MCF-10A breast epithelial cells by means of Alamar blue technique and Scratch assay.Results: Inflorescence extracts showed better bacteriostatic effects than leaf extracts on most bacteria, in both aqueous and hydroalcoholic extracts. The concentrated extract of spike inflorescence showed measurable activity with good effects on Gram-positive bacteria, but also on multidrug-resistant Gram-negative ones. The 70% methanolic extract of the species Prunella vulgaris L. (spike inflorescence) demonstrated a concentration-dependent cytotoxic and anti-migratory activity on MDA-MB-231 breast cancer cells, while affecting the non-tumor cell line less.Conclusions: The results suggest that Prunella vulgaris extracts present antibacterial potential in the complementary treatment of multidrug-resistant infections. The extract from the spike inflorescence of Prunella vulgaris L. produced a dose and time-dependent reduction in cell viability and migration, eliciting a stronger effect on the breast adenocarcinoma cell line.

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Antimicrobial activity of Prunella Vulgaris extracts against multi-drug resistant Escherichia Coli from patients of urinary tract infection

Cited by 20 publications

References 17 publications

Antibacterial effect of copper sulfate against multi-drug resistant nosocomial pathogens isolated from clinical samples

Antibacterial effect of copper sulfate against multi-drug resistant nosocomial pathogens isolated from clinical samples

Infecções Do Trato Urinário: Uma Revisão Sobre as Evidências Científicas Das Principais Plantas Medicinais Utilizadas Na Prática Clínica

Antibacterial and antitumor activity of the species Prunella vulgaris L.

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