Antimicrobial activity of Lippia sidoides Cham. (Verbenaceae) essential oil against Staphylococcus aureus and Escherichia coli

Abstract

ABSTRACT:The antibacterial effect of Lippia sidoides (rosemary pepper) essential oil was tested against the bacteria Staphylococcus aureus and Escherichia coli isolated from homemade Minas cheese produced in Brazil. The Minimum Inhibitory Concentration (MIC) determined in the Dilution Test was 13 L oil mL -1 for both bacteria, which characterizes inhibitory action in broth for a 24-hour interaction period. The Minimum Bactericidal Concentration (MBC) determined in the Suspension Test, with one minute of contact, was 25 L oil mL -1 for both tested bacteria, obtaining at this concentration a bactericidal effect of 99.9% on the viable bacterial cells from each sample. Results demonstrated the bacterial activity of Lippia sidoides essential oil against S. aureus and E. coli, suggesting its use as an antibacterial agent in foods.

Antimicrobial activity and acute and chronic toxicity of the essential oil of Lippia origanoides

“…Our results are in agreement with data in the literature, which show the bactericidal activity of the L. origanoides essential oil against various microorganisms (Veras et al 2014) and suggests that this concentration is most efficient in oils obtained from plants collected between the months of December and May. The results obtained in other studies using the essential oil of L. origanoides extracted from plants from the North of Minas Gerais also concur with the optimal concentration found in this study (Castro et al 2011, Costa et al 2011, Pinho et al 2012.…”

“…The antimicrobial effect of L. origanoides essential oil has been tested against the Staphylococcus aureus and Escherichia coli isolated from artisanal Minas cheese produced in Brazil (Castro et al 2011). The results showed the bactericidal activity of the essential oil of L. origanoides, suggesting the possibility of its use as an alternative antibacterial agent.…”

“…The results showed the bactericidal activity of the essential oil of L. origanoides, suggesting the possibility of its use as an alternative antibacterial agent. Other studies assessed the antimicrobial and antiseptic activity of L. origanoides essential oil on 25 strains of bacteria (coagulase-negative Staphylococcus, coagulase-positive Staphylococcus, esculin-positive Streptococcus, esculin-negative Streptococcus, and Mannheimia haemolytica) isolated from sheep with mastitis in the north region of Minas Gerais (Castro et al 2011). The antimicrobial activity of the L. origanoides extract was also tested on isolates of S. aureus in which inhibition of microbial growth was shown (Oliveira et al 2006a(Oliveira et al , 2006b).…”

Atividade antisséptica do óleo essencial de Lippia origanoides Cham. (Alecrim-pimenta) na presença de leite bovino

“…Estudos realizados com óleo essencial de alecrim-pimenta provenientes da mesma área de reserva nativa do ICA-UFMG na região Norte de Minas Gerais apresentaram resultados semelhantes quanto aos compostos majoritários, (10) carvacrol, seguido de (03) p-cimeno, (7) timilmetil éter, (11) cariofileno e o γ-terpineno (05) (Costa et al 2011, Castro et al 2011, Queiroz et al 2014. Resultados relatados por autores em outras regiões do estado de Minas Gerais também são condizentes com os aqui descritos, ainda que os compostos tenham sido encontrados em percentual variável.…”

“…O potencial uso deste óleo essencial como antimicrobiano e antisséptico em saúde e produção animal, já foi confirmado in vitro em bactérias isoladas de leite e outros alimentos de origem animal (Costa et al 2011, Castro et al 2011, Queiroz et al 2014 , no entanto, pesquisas que avaliem a possível interferência de matéria orgânica na atividade ainda não foram publicadas.…”