Antimicrobial Activity of Isoniazid in Conjugation with Surface-Modified Magnetic Nanoparticles against Mycobacterium tuberculosis and Nonmycobacterial Microorganisms

Abstract

Isoniazid, the choice antitubercular agent, has only been employed against Mycobacterium tuberculosis. This study evaluated if the enzyme-mimetic activities of magnetic nanoparticles could accelerate the activation process of isoniazid against mycobacterial and, more importantly, non-mycobacterial microorganisms. First, magnetic nanoparticles were synthesized and coated by lipoamino acid; then, isoniazid was conjugated to synthesized nanoparticles. Antibacterial activities of nanoconjugated isoniazid were evaluated against Mycobacterium tuberculosis and four Gram-positive and Gram-negative nonmycobacterial strains through a microdilution broth process. Results showed that the required amount of isoniazid against Mycobacterium tuberculosis would decrease to 44.8% and 16.7% in conjugation with naked and surface-modified magnetic nanoparticles, respectively. Also, 32 μg/mL and 38 μg/mL of isoniazid in conjugation with naked and surface-modified nanoparticles, respectively, could prevent the growth of Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus. Hence, the vicinity of magnetic nanoparticles with isoniazid could declare promising aspects of isoniazid antibacterial capabilities.

Bioinorganic Synthesis of Polyrhodanine Stabilized Fe3O4/Graphene Oxide in Microbial Supernatant Media for Anticancer and Antibacterial Applications

“…Although the antimicrobial effects of GO/Fe nanocomposite have been studied in few studies, many recent studies have examined the antimicrobial effects of GO nanocomposites with other inorganic materials, especially silver nanoparticles [ 23 , 34 , 35 ]. However, it has been repeatedly shown that the antimicrobial effects of magnetic nanoparticles are negligible [ 28 , 36 , 37 ], and the antimicrobial results of this study are comparable to the results of GO/silver nanocomposites. These effects, which are naturally less than GO/silver nanocomposites, could be due to the added effect of natural compounds attached to the nanoparticle surface due to bioinorganic synthesis.…”

“…is experiment was designed for MFC value calculation when the fungal strain was the culture in the RPMI medium. e lowest concentration value of the sample, which causes less than four Bioinorganic Chemistry and Applications visible colonies, was considered MBC for all bacterial strains and MFC for fungal strain [28,29]. ese tests were accomplished in triplicate.…”

Antibacterial Effects of Green-Synthesized Silver Nanoparticles Using Ferula asafoetida against Acinetobacter baumannii Isolated from the Hospital Environment and Assessment of Their Cytotoxicity on the Human Cell Lines

“…So far, many attempts have been designed to distinguish the effect of silver nanoparticles against various tumors. One of the broadly acknowledged hypotheses has ascribed the potential generation of reactive oxygen species created by nanoparticles and their ions, preventing cell growth and eventually cell apoptosis [8,53].…”

“…They exhibit anti-inflammatory, antioxidant, antitumor, and antimicrobial properties, leading to their broad applications in biomedicine [7]. Overall, the biogenic synthesis of NPs is considered a valuable strategy by providing more profitable NPs with higher stability and biocompatibility [8].…”

From Genome to Drugs: New Approaches in Antimicrobial Discovery

“…Although antimicrobial activity of INH is thought to be selective for mycobacteria, likely due to its ability to inhibit mycolic acid synthesis, Bb FabI and Mtb InhA (the protein target of INH) share the same and domains and are structural homologs, except for the presence of a long loop of interaction with the substrate found in InhA ( Andrade et al, 2008 ). Moreover, it was recently shown that isoniazid in conjugation with nanoparticles could prevent the growth of Enterococcus faecalis, E. coli, Pseudomonas aeruginosa, and S. aureus ( Zargarnezhad et al, 2020 ). This prodrug is activated by the heme enzyme catalase-peroxidase (KatG) endogenous to M. tuberculosis.…”