Antimicrobial Activity of Chitosan Membranes Against Staphylococcus Aureus of Clinical Origin

Escárcega-Galaz, Ana A.; López‐Cervantes, Jaime; Sánchez-Machado, Dalia I.; Brito-Zurita, Olga Rosa; Campas‐Baypoli, Olga N.

doi:10.5772/65980

Cited by 6 publications

(6 citation statements)

References 35 publications

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“…Tannic acid spectra were consistent with values given by other studies [28]. Its FTIR spectrum exhibited characteristic bands of aromatic rings in the wavenumber range of 1445-1698 cm −1 .…”

Section: Fourier Transform Infrared Spectroscopysupporting

confidence: 90%

Tannic Acid Tailored-Made Microsystems for Wound Infection

Guimarães

Costa

Madureira

et al. 2023

IJMS

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Difficult-to-treat infections make complex wounds a problem of great clinical and socio-economic impact. Moreover, model therapies of wound care are increasing antibiotic resistance and becoming a critical problem, beyond healing. Therefore, phytochemicals are promising alternatives, with both antimicrobial and antioxidant activities to heal, strike infection, and the inherent microbial resistance. Hereupon, chitosan (CS)-based microparticles (as CM) were designed and developed as carriers of tannic acid (TA). These CMTA were designed to improve TA stability, bioavailability, and delivery in situ. The CMTA were prepared by spray dryer technique and were characterized regarding encapsulation efficiency, kinetic release, and morphology. Antimicrobial potential was evaluated against methicillin-resistant and methicillin-sensitive Staphylococcus aureus (MRSA and MSSA), Staphylococcus epidermidis, Escherichia coli, Candida albicans, and Pseudomonas aeruginosa strains, as common wound pathogens, and the agar diffusion inhibition growth zones were tested for antimicrobial profile. Biocompatibility tests were performed using human dermal fibroblasts. CMTA had a satisfactory product yield of ca. 32% and high encapsulation efficiency of ca. 99%. Diameters were lower than 10 μm, and the particles showed a spherical morphology. The developed microsystems were also antimicrobial for representative Gram+, Gram−, and yeast as common wound contaminants. CMTA improved cell viability (ca. 73%) and proliferation (ca. 70%) compared to free TA in solution and even compared to the physical mixture of CS and TA in dermal fibroblasts.

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Section: Fourier Transform Infrared Spectroscopysupporting

confidence: 90%

Tannic Acid Tailored-Made Microsystems for Wound Infection

Guimarães

Costa

Madureira

et al. 2023

IJMS

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“…In addition, it was possible to isolate Candida albicans with a prevalence of 19.35 %. Previous studies related to gram-positive bacteria were published, particularly with regard to S. aureus (Escárcega-Galaz et al 2017). Specifically, this work focused on gram-negative bacteria.…”

Section: Phenotypic Identification Of Microorganisms In Skin Ulcersmentioning

confidence: 99%

Effect of chitosan membranes against gram-negative bacteria isolated from cutaneous ulcers

López‐Cervantes

Escárcega-Galaz

Sánchez‐Machado

et al. 2021

Nova Biotechnol. Chim.

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The objective of this study was to evaluate the in vitro effectiveness of membranes developed with pure chitosan and chitosan in a mixture with glycerol-honey against gram-negative bacteria isolated from skin ulcers. The membranes were prepared by the solvent evaporation technique. The identification and antibiotic sensitivity of microorganisms were determined in microplates, and in vitro tests were developed by the agar diffusion technique. The most frequently isolated microorganism was Escherichia coli with 43.75 % prevalence. All membranes showed antimicrobial effects by direct contact against Proteus mirabilis, Escherichia coli, Enterobacter aerogenes, Pseudomonas aeruginosa, Klebsiella pneumoniae and Morganella morganii. Antibiograms showed that most of these microorganisms are multi-resistant to antibiotics. All of this suggests that chitosan-based membranes are a safe alternative for the treatment of infected cutaneous ulcers compared to traditional antibiotics. The outcomes of this study confirm that membranes made of a biodegradable polymer, such as chitosan have activity against multidrug-resistant gram-negative bacteria that grow in infected skin ulcers.

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“…mast nebo náplast na zevní potírání akné bez vedlejších účinků léčby jako je tomu v případě antibiotik či kortikoidů. Podobně mohou být likvidovány i vůči antibiotikům odolné bakteriální kmeny, jako jsou některé druhy Escherichia coli nebo Staphylococcus aureus (34,35).…”

Section: Užití Chitosanu Jako Jednoho Z Moderních úSpěchů Biomedicínsunclassified