Antimicrobial Activity, Antioxidant Properties and Phytochemical Screening of Aesculus hippocastanum Mother Tincture against Food-borne Bacteria

Bonomo, Maria Grazia; Cafaro, Caterina; Russo, Daniela; Calabrone, Luana; Milella, Luigi; Saturnino, Carmela; Capasso, Anna; Salzano, Giovanni

doi:10.2174/1570180816666181108114115

Cited by 22 publications

(25 citation statements)

References 18 publications

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“…In phytochemical assay, our study matches with [56] who reported that the methanol extract of different varieties of pomegranate had the highest phenolic content. The total phenolic content, total flavonoids, and tannins showed a proportional relationship with antioxidant values, as already observed by other authors [62]. The total phenolic content of methanolic and ethanolic extract of orange peels in this study (18.61 and 17.704 mg GAE/ml) was higher than that of [51] for the same extract (12.5 and 10.25mg GAE/ml).…”

Section: Discussionsupporting

confidence: 89%

Chemical profiling, in vitro antimicrobial and antioxidant activities of pomegranate, orange and banana peel-extracts against pathogenic microorganisms

Hanafy¹,

El-Shafea²,

Saleh

et al. 2021

Journal of Genetic Engineering and Biotechnology

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Background The use of natural preservatives became of great interest; good examples of these natural preservation agents are plant peels. The use of plant peels has dual benefits; first is their antimicrobial activity against food-borne pathogens, while the second is minimizing agro-industrial wastes. Results The evaluation of the antimicrobial potential of both methanolic and ethanolic extracts of three fruit peels (orange, pomegranate, and banana), against 4 Gram-positive (G+), 3 Gram-negative bacteria (G−), and 2 fungal strains revealed that both pomegranate peel extracts exhibited significantly higher inhibitory effect on all tested G+ bacteria. Methanolic extract of pomegranate peel gave higher activity than the ethanolic one against G+ and G− bacteria except for S. typhimurium. Against A. flavus and A. niger, both pomegranate and orange extracts showed activity ranging between 65 and 100% more than the positive control. The ethanolic extracts of all tested peels showed a considerable capacity of antioxidant compounds compared to the methanolic extracts. The highest antioxidant capacity was found for ethanolic and methanolic extracts of pomegranate, 66.870 and 56.262 mg/ml, respectively. Generally, the concentration of total phenolic compounds was higher than that of total flavonoids followed by tannins. The highest readings of all tested constituents were reported for pomegranate extracts followed by orange and then banana. The total phenolic content, total flavonoids, and tannins were proportional to antioxidant values. GC-MS of pomegranate peel extracts identified 23 compounds in the methanolic extract versus 31 compounds in the ethanolic one. These components were identified based on their retention times and mass spectral fragmentation pattern. 5-hydroxymethylfufural (HMF) represented the major component in both methanolic and ethanolic extracts with peak area percentage of 65.78% and 48.43%, respectively. Conclusions The results showed negative effect of methanolic and ethanolic extracts of pomegranate on G+ and G− bacteria and two fungal pathogenic strains. The phytochemical analysis regarded these results to the high content of phenols, flavonoids, and tannins. GC-MS chromatogram identified many compounds known to be effective as antioxidants and antibacterial and antifungal agents. These indications show that pomegranate peel may be a superior natural food-preserver, but further studies about the suitable formulation, dosage, and possible side-effects are still needed.

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Section: Discussionsupporting

confidence: 89%

Chemical profiling, in vitro antimicrobial and antioxidant activities of pomegranate, orange and banana peel-extracts against pathogenic microorganisms

Hanafy¹,

El-Shafea²,

Saleh

et al. 2021

Journal of Genetic Engineering and Biotechnology

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“…There are also some studies in the literature regarding the antioxidant activity potential of this species. In a study investigating the antioxidant activity of A. hippocastanum mother tincture (TM), it was reported that the polyphenolic content of the sample was determined to be 506.8 mg GAEs/100 mL and exhibited significant antioxidant activity [43]. In another study evaluating the antioxidant activity of flower extracts obtained from A. hippocastanum, it was determined that the IC 50 value measured as a result of the FRAP assay was 159.82 µg/mL and a dose-related decrease in reactive oxygen species production have been determined [6].…”

Section: Antioxidant Activitymentioning

confidence: 99%

Enzyme and Biological Activities of the Water Extracts from the Plants Aesculus hippocastanum, Olea europaea and Hypericum perforatum That Are Used as Folk Remedies in Turkey

et al. 2020

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Phenolic compounds are secondary metabolites that are found ubiquitously in plants, fruits, and vegetables. Many studies have shown that regular consumption of these compounds could have a positive effect on our health. The aim of this study was to compare the phytochemical contents of the water extracts from three different plants used as folk remedies in Turkey: Aesculus hippocastanum, Olea europaea, and Hypericum perforatum. A liquid chromatography-electrospray tandem mass spectrometry (LC-ESI-MS/MS) analysis was performed to explore the phenolic profiles. The biological activities of these extracts were also evaluated in terms of their antioxidant activities (2,2-diphenyl-1-picrylhydrazyl DPPH, 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid ABTS, Ferric Reducing Antioxidant Power Assay FRAP, cupric ion reducing antioxidant capacity CUPRAC, β-carotene, phosphomolybdenum, and metal chelating) and enzyme inhibitory properties (against acetylcholinesterase, butyrylcholinesterase, and tyrosinase). The aqueous extract of H. perforatum showed the highest levels of total phenolic, flavonoid, and saponin contents. Protocatechuic acid, vanillic acid, verbascoside, hesperidin, hyperoside, apigenin 7-hexosides, and quercetin were the most common compounds found in this species. The results confirm that A. hippocastanum, O. europaea, and H. perforatum represent a potential source of natural-derived molecules with positive properties that could be used as valid starting point for new food supplements, and drugs in the pharmaceutical, cosmetic, and food industries.

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“…A Aesculus hippocastanum L. (família Sapindaceae) corresponde a uma árvore robusta popularmente conhecida como castanheiro-da-índia (Colson et al, 2019). Os extratos de suas sementes que são chamadas de castanha-da-índia, são utilizados há muitos séculos com fim terapêutico, sendo especialmente empregadas no tratamento de insuficiência venosa crônica, ulcerações, queimaduras e tratamento de inflamações na pele (Bonomo et al, 2020).…”

Section: De Acordo Comunclassified

Avaliação físico-química e microbiológica de fitoterápicos utilizados em uma farmácia municipal de manipulação

Meotti

Silva

Gumy

et al. 2021

RSD

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O medicamento fitoterápico possui como insumo, plantas medicinais ou seus derivados. Apesar de inúmeros benefícios, o uso indiscriminado e falhas durante a produção podem expor o consumidor final a efeitos nocivos à sua saúde. Nesse sentido, o estudo avaliou a qualidade físico-química e microbiológica de insumos vegetais utilizados em uma farmácia de manipulação municipal na região sudoeste do Paraná. Foram analisadas amostras de extratos secos de: Cymbopogon citratus, Baccharis trimera., Aesculus hippocastanum, Equisetum arvense, Echinodorus grandiflorus, Curcuma longa, Ginkgo biloba, Piper methysticum, Melissa officinalis e Passiflora incarnata. Para a avaliação físico-química, foram realizados os testes, segundo a Farmacopeia Brasileira (2019): características organolépticas, umidade, granulometria, pH, densidade, solubilidade e constituintes secundários, dentre os quais flavonoides, saponinas e taninos. Para a análise microbiológica, determinou-se a presença de microrganismos totais, bolores, leveduras, Salmonella sp e Shiguella sp, segundo Barbosa (2014). A avaliação organoléptica revelou que todas as amostras se apresentaram com aspecto de pó fino e homogêneo, com odor e cor característicos de cada espécie. Os extratos secos apresentaram resultados de teor de umidade, granulométricos, pH e densidade dentro dos valores de referência farmacopéicos. Com relação a solubilidade, a amostra de E. arvense se revelou praticamente insolúvel ao solvente utilizado. Todas as amostras testaram positivo para a presença de flavonoides. As amostras de G. biloba, P. methysticum e P. incarnata não apresentaram saponinas. Nenhuma amostra apresentou contaminação por bactérias totais, Salmonella sp e Shiguella sp. Portanto, os resultados obtidos nesta pesquisa atendem aos parâmetros da legislação vigente, comprovando a qualidade dos extratos estudados.

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Antimicrobial Activity, Antioxidant Properties and Phytochemical Screening of Aesculus hippocastanum Mother Tincture against Food-borne Bacteria

Cited by 22 publications

References 18 publications

Chemical profiling, in vitro antimicrobial and antioxidant activities of pomegranate, orange and banana peel-extracts against pathogenic microorganisms

Chemical profiling, in vitro antimicrobial and antioxidant activities of pomegranate, orange and banana peel-extracts against pathogenic microorganisms

Enzyme and Biological Activities of the Water Extracts from the Plants Aesculus hippocastanum, Olea europaea and Hypericum perforatum That Are Used as Folk Remedies in Turkey

Avaliação físico-química e microbiológica de fitoterápicos utilizados em uma farmácia municipal de manipulação

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