Antimicrobial Activity and Stability of Weakly Acidified Chlorous Acid Water

Horiuchi, Isanori; Kawata, Hiroyuki; Nagao, Taeko; Imaohji, Haruyuki; Murakami, Kazuya; Kino, Yasuhiro; Yamasaki, Hisashi; Koyama, A. Hajime; FUJITA, Yatsuka; Goda, Hisataka; Kuwahara, Tomomi

doi:10.4265/bio.20.43

Cited by 23 publications

(27 citation statements)

References 25 publications

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“…For inactivation of microorganisms in spilled body fluids such as blood and feces, although at these concentrations the compound may produce carcinogens and poisonous substances resulting from its reaction with organic molecules. On the other hand, Hydrochlorous acid HOCl solution pH 6 is superior to ClO -solution in terms of higher microbicidal activity against a broad range of microorganisms Horiuchi et al, 2015 . However, HOCl is less stable to various environmental factors than is ClO -.…”

mentioning

confidence: 99%

“…Given this reagent s antimicrobial activity and low cost, ClO -is widely used for sanitation and decontamination in various fields, including healthcare and food processing Fukuzaki et al, 2006, Ardizzoni et al, 2009, Horiuchi et al, 2015 -has relatively poor antimicrobial activities against some microorganisms such as Aspergillus oryzae, bacterial spores, poliovirus, and norovirus. High concentrations >1000 ppm of ClO -are generally recommended in clinical settings for the decrease of residual chlorine levels were also evaluated FIG.1B .…”

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confidence: 99%

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Stability of Weakly Acidic Hypochlorous Acid Solution with Microbicidal Activity

et al. 2017

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Stability of Weakly Acidic Hypochlorous Acid Solution with Microbicidal Activity

et al. 2017

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“…Chlorous acid (HClO 2 )-based sanitizers such as acidified sodium chlorite solution (ASC) and weakly acidified chlorous acid water (WACAW) have been applied to food and environmental sanitation [23–26]. HClO 2 -based sanitizers have been reported to be more stable than NaClO under organic-matter-rich conditions [24, 26, 27].…”

Section: Introductionmentioning

confidence: 99%

“…HClO 2 -based sanitizers have been reported to be more stable than NaClO under organic-matter-rich conditions [24, 26, 27]. They contain various forms of chlorinated oxides such as HClO 2 , dissolved ClO 2 and ClO 2 - , and the former two are responsible for most of the microbicidal activity of WACAW.…”

Section: Introductionmentioning

confidence: 99%

Microbicidal effects of weakly acidified chlorous acid water against feline calicivirus and Clostridium difficile spores under protein-rich conditions

Goda¹,

Yamaoka

Nakayama‐Imaohji

et al. 2017

PLoS ONE

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Sanitation of environmental surfaces with chlorine based-disinfectants is a principal measure to control outbreaks of norovirus or Clostridium difficile. The microbicidal activity of chlorine-based disinfectants depends on the free available chlorine (FAC), but their oxidative potential is rapidly eliminated by organic matter. In this study, the microbicidal activities of weakly acidified chlorous acid water (WACAW) and sodium hypochlorite solution (NaClO) against feline calcivirus (FCV) and C. difficile spores were compared in protein-rich conditions. WACAW inactivated FCV and C. difficile spores better than NaClO under all experimental conditions used in this study. WACAW above 100 ppm FAC decreased FCV >4 log10 within 30 sec in the presence of 0.5% each of bovine serum albumin (BSA), polypeptone or meat extract. Even in the presence of 5% BSA, WACAW at 600 ppm FAC reduced FCV >4 log10 within 30 sec. Polypeptone inhibited the virucidal activity of WACAW against FCV more so than BSA or meat extract. WACAW at 200 ppm FAC decreased C. difficile spores >3 log10 within 1 min in the presence of 0.5% polypeptone. The microbicidal activity of NaClO was extensively diminished in the presence of organic matter. WACAW recovered its FAC to the initial level after partial neutralization by sodium thiosulfate, while no restoration of the FAC was observed in NaClO. These results indicate that WACAW is relatively stable under organic matter-rich conditions and therefore may be useful for treating environmental surfaces contaminated by human excretions.

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“…log N/N 0 = -kCT 2 where N and N 0 are the number of surviving cells at time T and time zero, min, respectively; C is the NaClO 2 concentration mM , and k is the first-order inactivation ASC , which is produced by the addition of food-gradeacid such as citric acid and phosphoric acid to NaClO 2 solution, has been demonstrated to have an excellent antimicrobial effect Benford et al, 2008;Horiuchi et al, 2015 . Acidification of NaClO 2 solution results in the protonation of the chlorite ion ClO 2 -to chlorous acid HClO 2 .…”

Section: Inactivation Of V Parahaemolyticus By Naclo 2 Solutionmentioning

confidence: 99%

Kinetics of the Inactivation of <i>Vibrio parahaemolyticus</i> in Weakly Acidic Sodium Chlorite Solution

2017

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The kinetics of the inactivation of Vibrio parahaemolyticus in sodium chlorite NaClO 2 solution was studied in the weakly acidic pH range of 4.0 to 6.5 and at various temperatures. The logarithmic reduction of the survival ratio depended on the concentration-time product, and all the inactivation curves showed a linear reduction phase. The first-order inactivation rate constant k increased by approximately twice for every 0.44 unit fall in pH. During the inactivation experiments, no formation of chlorine dioxide occurred. These data indicated that undissociated HClO 2 was the active species governing the inactivation of V. parahaemolyticus. It was also shown that the use of weakly acidic NaClO 2 solutions containing high concentrations of ionized ClO 2 -gave slower kinetics of the inactivation, whereas it could achieve the significant reduction of viable cells of more than 4-log. The k value showed an Arrhenius-type temperature dependence in the temperature range of 5 to 40 . The apparent activation energy for the inactivation of V. parahaemolyticus was estimated to be 43.5 kJ/mol. The k value increased by approximately 1.8 times for every 10 rise in temperature.

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Antimicrobial Activity and Stability of Weakly Acidified Chlorous Acid Water

Cited by 23 publications

References 25 publications

Stability of Weakly Acidic Hypochlorous Acid Solution with Microbicidal Activity

Stability of Weakly Acidic Hypochlorous Acid Solution with Microbicidal Activity

Microbicidal effects of weakly acidified chlorous acid water against feline calicivirus and Clostridium difficile spores under protein-rich conditions

Kinetics of the Inactivation of <i>Vibrio parahaemolyticus</i> in Weakly Acidic Sodium Chlorite Solution

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