Antimicrobial Activities of Lactic Acid Bacteria Strains Isolated from Human Breast Milk Against Human Pathogenic Strains

Abstract

This study aims to screen the antimicrobial activity of lactic acid bacteria (LAB) with probiotic properties isolated from human breast milk. A total of six from twenty five LAB isolated showed clear zone on modified MRS-CaCO 3 agar, catalase negative and Gram positive were considered as LAB. All of the six selected isolates were able to tolerance pH 2, 0.3% bile salts for 3h. The antibacterial properties of these isolates against (Escherichia coli, Bacillus subtilis, Staphylococcus aureus and Salmonella typhimurium) were examined using dual agar overlay and microtiter plates methods. Results found that both the cells and supernatants of six selected LAB isolated showed very good inhibitory activity against the target bacteria. The LAB-HM6 isolate showed the highest inhibitory activity (32.0 mm) against S. aureus followed by LAB-HM5 and LAB-HM4 (30.3 mm), then LAB-MH1 (29.3 mm) against S. typhimurium. Supernatant LAB-HM5 caused complete inhibition of all target bacteria, LAB-HM3 inhibited S. typhimurium and B. subtilis, and LAB-HM5 also inhibited the growth of S. aureus during 72 h incubation. Thus, these Lab isolates could be considered as potential antimicrobial probiotic strains human pathogens and should be further studied for their human health benefits.

Evaluation of Lactobacillus spp. isolated from locally consumed probiotic food in Nsukka, Enugu State, Nigeria for antimicrobial activity utilizing agar well diffusion and pH tolerance tests

“…Isolate -Y 1 had the broadest activity inhibiting all the pathogens except antimicrobial activity of Lactobacilli against enteropathogens are corroborated by another author who reported their in vitro inhibitory activity on Bacillus sp., P. aeruginosa, Staph. aureus, E. coli and S. typhimurium (Abubakr, 2018). Among the pathogens tested, Bacillus sp.…”

Human milk as a potential source for isolation of probiotic lactic acid bacteria: a mini review

“…If the process is being interrupted, there will be higher chances of diseases in later life. Breast milk is considered as the major contributing postnatal factor in microbiome, metabolome and immunological programming (Amenu, 2014;Abubakr, 2018). A large body of literature has investigated the effect of human milk on the health of infants; however, few writers have been able to draw on any systematic research into the microbiology of breast milk.…”