Antiinflammatory, antioxidant and cytotoxic actions of β-glucan-rich extract from Geastrum saccatum mushroom

Dore, Celina Maria Pinto Guerra; Azevedo, Tarciana Carvalho Gurgel de; Souza, Micheline Cristiane Rocha de; Rego, Leonardo Augusto R. de Souza; Dantas, Julio C.M. de; Silva, Fernando R.F.; Rocha, Hugo Alexandre Oliveira; Baseia, Iuri Goulart; Leite, Edda Lisboa

doi:10.1016/j.intimp.2007.04.010

Cited by 98 publications

(24 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Body weight of individual animal was recorded before and at the end of experiment. After 24 hours of the administration of the last dose, the animals were euthanized by anesthetizing with ketamine hydrochloride and xylazine hydrochloride administered [24]. Whole blood was then sampled from the retro-orbital sinus with suitable hematological tube.…”

Section: Methodsmentioning

confidence: 99%

Gracilaria edulis extract induces apoptosis and inhibits tumor in Ehrlich Ascites tumor cells in vivo

Patra

Muthuraman

2013

BMC Complement Altern Med

View full text Add to dashboard Cite

BackgroundMarine environment is inestimable for their chemical and biological diversity and therefore is an extraordinary resource for the discovery of new anticancer drugs. Recent development in elucidation of the mechanism and therapeutic action of natural products helped to evaluate for their potential activity.MethodsWe evaluated Gracilaria edulis J. Ag (Brown algae), for its antitumor potential against the Ehrlich ascites tumor (EAT) in vivo and in vitro. Cytotoxicity evaluation of Ethanol Extract of Gracilaria edulis (EEGE) using EAT cells showed significant activity. In vitro studies indicated that EEGE cytotoxicity to EAT cells is mediated through its ability to produce reactive oxygen species (ROS) and therefore decreasing intracellular glutathione (GSH) levels may be attributed to oxidative stress.ResultsApoptotic parameters including Annexin-V positive cells, increased levels of DNA fragmentation and increased caspase-2, caspase-3 and caspase-9 activities indicated the mechanism might be by inducing apoptosis. Intraperitoneally administration of EEGE to EAT-bearing mice helped to increase the lifespan of the animals significantly inhibited tumor growth and increased survival of mice. Extensive hematology, biochemistry and histopathological analysis of liver and kidney indicated that daily doses of EEGE up to 300 mg/kg for 35 days are well tolerated and did not cause hematotoxicity nor renal or hepatotoxicity.ConclusionComprehensive antitumor analysis in animal model and in Ehrlich Ascites Tumor cells was done including biochemical, and pathological evaluations indicate antitumor activity of the extract and non toxic in vivo. It was evident that the mechanism explains the apoptotic activity of the algae extract.

show abstract

Section: Methodsmentioning

confidence: 99%

Gracilaria edulis extract induces apoptosis and inhibits tumor in Ehrlich Ascites tumor cells in vivo

Patra

Muthuraman

2013

BMC Complement Altern Med

View full text Add to dashboard Cite

show abstract

“…Among these polysaccharides are xyloglucan, mannogalactan and ␤-d-glucan [3][4][5]. Interestingly, a variety of biological effects have been ascribed to ␤-d-glucans, namely antitumoral, antioxidant, anti-inflammatory and immunomodulatory properties [6][7][8][9]. Recently, our group reported that (1→3),(1→6)-linked ␤-d-glucan (GL) isolated from P. pulmonarius attenuated nociceptive responses, as well as inhibited leukocyte migration and plasma extravasation induced by acetic acid in mice [4].…”

Section: Introductionmentioning

confidence: 99%

Antinociception of β-d-glucan from Pleurotus pulmonarius is possibly related to protein kinase C inhibition

Baggio

Freitas

Marcon

et al. 2012

International Journal of Biological Macromolecules

View full text Add to dashboard Cite

β-D-Glucan, a polysaccharide isolated from an edible mushroom Pleurotus pulmonarius (Fr.) Quel., presented antinociceptive activity in mice. This study evaluated the involvement of transient receptor potential (TRP) channels and protein kinase C (PKC) on antinociceptive effect of a (1→3),(1→6)-linked β-D-glucan (GL) in mice. Intraperitoneal administration of GL potently inhibited nociceptive responses induced by intraplantar injections of capsaicin, cinnamaldehyde, menthol, acidified saline and phorbol myristate acetate (PMA). Moreover, Western blot analysis revealed that GL treatment also prevented PMA-induced PKCɛ activation. Collectively, present results demonstrate that GL could constitute an attractive molecule of interest for the development of new analgesic drugs.

show abstract

“…Subcutaneous formalin administration in rats' paw gen-ture, among them the inhibition of cycloxygenase and nitric oxide synthase 10 . In addition to the anti-inflammatory action, the 2-amino-3H-phenoxazine-3-ona compound, obtained from Agaricus bisporus Imbach, has inhibited nitric oxide and prostaglandin E2 production in macrophages after activation with LPS and gamma interferon, in addition to inhibiting cycloxygenase 1 and 2 activity.…”

Section: Discussionmentioning

confidence: 99%

Antinociceptive and anti-inflammatory effect of Agaricus blazei Murill in rats submitted to the modified formalin test

Carvalho

Alves

Monteiro³

et al. 2011

Rev. dor

View full text Add to dashboard Cite

Sociedade Brasileira para o Estudo da Dor c Rev Dor. São Paulo, 2011 jan-mar;12(1):35-8 SUMMARY BACKGROUND AND OBJECTIVES:The consumption of mushrooms in popular and traditional medicine is associated to immune modulating activity. It is known that decreasing the inflammatory process will decrease nociception. This study aimed at checking the antinociceptive and anti-inflammatory activity of Agaricus blazei Murill, mushroom of the sun, in Wistar rats using the modified formalin test. METHOD: Animals (n = 18) were distributed in the following groups: Control Group (CG): non-treated animals receiving just 2% Tween solvent (n = 6); Treated Group (TG): animals daily treated orally with 2.65 mg of A. blazei powder in 2% Tween solvent for 15 days (n = 12). After treatment, they were submitted to the modified formalin test. RESULTS: After formalin injection, the three phases of the formalin test were observed in CG and TG animals. The treatment with A. blazei has not interfered with formalin test phases I or II, but has decreased the number of paw elevations in phase III. CONCLUSION: The treatment with Agaricus blazei during 15 days had action in nociceptive response and acute inflammation because rats treated with Agaricus had a lower number of paw movements during phase III,

show abstract

Antiinflammatory, antioxidant and cytotoxic actions of β-glucan-rich extract from Geastrum saccatum mushroom

Cited by 98 publications

References 28 publications

Gracilaria edulis extract induces apoptosis and inhibits tumor in Ehrlich Ascites tumor cells in vivo

Gracilaria edulis extract induces apoptosis and inhibits tumor in Ehrlich Ascites tumor cells in vivo

Antinociception of β-d-glucan from Pleurotus pulmonarius is possibly related to protein kinase C inhibition

Antinociceptive and anti-inflammatory effect of Agaricus blazei Murill in rats submitted to the modified formalin test

Contact Info

Product

Resources

About