Antihyperglycaemia and related gene expressions of aqueous extract ofGongronema latifoliumleaf in alloxan-induced diabetic rats

Abstract

Context: Gongronema latifolium Benth (Asclepiadaceae) has been highly utilized in controlling diabetes mellitus traditionally in the eastern part of Nigeria.Objectives: Antihyperglycaemic and related gene expressions of aqueous extract of Gongronema latifolium leaf in alloxan-induced diabetic rats.Materials and methods: Forty-eight female Wistar rats were induced intraperitoneally using alloxan (150 mg/kg body weight). The rats were separated into six groups (n = 8) as follows: non-diabetic control, diabetic control, diabetic rats administered 5 mg/kg body weight of metformin, and diabetic rats administered 6.36, 12.72 and 25.44 mg/kg body weight (ethnobotanical doses) of G. latifolium orally daily. On the 14th day, the animals were sacrificed and different antihyperglycaemic parameters were evaluated as well as its related gene expressions.Results: Diabetic rats administered three doses of aqueous extract of G. latifolium significantly (p < 0.05) lowered the fasting blood glucose, glycated haemoglobin, serum lipid profiles, lipid peroxidation (5.62–1.2 μ/mg protein) levels, as well as gene expression of glucose-6-phosphatase in alloxan-induced diabetic rats. There was a significant (p < 0.05) increase in the liver glycogen content (16.23–112.5 mg glucose/2 g), antioxidant enzymes activities, glucose transporter (GLUT-2 and GLUT-4) levels and relative gene expression of hexokinase in diabetic rats administered different doses of aqueous extract of G. latifolium.Discussion and conclusions: It can be deduced that the aqueous extract of G. latifolium leaf at these doses may be useful in managing diabetes mellitus and its associated complications. Therefore, this extract may be a potent antidiabetic agent in clinical therapy in the future.

<i>Sterculia tragacantha</i> Lindl Aqueous Leaf Extract Ameliorate Cardiomyopathy in Streptozotocin-induced Diabetic Rats via Urotensin II and FABP3 Expressions

“…In another word, diabetic animals showed a reduction in cardiac GSH, designating its excessive usage in the redox-tackled cellular vicinity 19) . SOD, an enzyme involved in the dismutation of superoxide, CAT and GPx are enzymes that catalyze the breakdown of hydrogen peroxide (free radical) to water and oxygen 20) , and GST is a detoxification enzyme that functions in safeguarding cellular macromolecules from attack by reactive electrophiles 2) . The levels of all these (enzymatic and non-enzymatic) were noticeably increased in diabetic animals treated with the two doses of ST leaf, which were drastically reduced in diabetic rats, supporting their roles in scavenging the generated ROS due to STZ.…”

“…Diabetes mellitus (DM) is a metabolic malady tagged with persistent hyperglycemia resulting in instabilities of carbohydrate, protein, and lipid metabolisms because of flaws in insulin secretion, insulin action, or both 1) . This triggers a series of clinical signs such as polyuria, polydipsia, polyphagia, and abnormal weight loss, amongst others 2) . Currently, more than 422 million people are suffering from this disease globally, with a death rate of over 4 million each year 2) .…”

“…This triggers a series of clinical signs such as polyuria, polydipsia, polyphagia, and abnormal weight loss, amongst others 2) . Currently, more than 422 million people are suffering from this disease globally, with a death rate of over 4 million each year 2) .…”

Screening of potential antidiabetic phytochemicals from Gongronema latifolium leaf against therapeutic targets of type 2 diabetes mellitus: multi-targets drug design

“…Glide) to find the best ligand that fits into the binding pocket of a receptor at the atomic and sub-atomic levels, thereby simulating intermolecular interaction between them [21,22]. Recent studies have shown the antidiabetic properties of Gongronema latifolium in rat model [23][24][25]. In order to investigate the binding capacity of the bioactive compound in Gongronema latifolium leaf on proteins related to diabetes in humans, the fractions of Gongronema latifolium leaf consisting of different phytochemicals were docked with fifteen protein targets associated with type 2 DM to discover the possible hit compounds.…”

Biochemical and Hepatoprotective Effects of Gangronema latifolium Extract on Alloxan-Induced Diabetic Rats

“…1 Recently, there has been an increase in plant-based therapeutic products in both developed and developing countries because they are mostly non-toxic, have fewer side effects, and are available at affordable prices. 2,3 One such medicinal plant is Gongronema latifolium. The plant belongs to the family of Asclepiadacae.…”