Antigenotoxic effects of Citrus aurentium L. fruit peel oil on mutagenicity of two alkylating agents and two metals in the Drosophila wing spot test

Abstract

Antigenotoxic effects of Citrus aurentium L. (Rutaceae) fruit peel oil (CPO) in combination with mutagenic metals and alkylating agents were studied using the wing spot test of D. melanogaster. The four reference mutagens, potassium dichromate (K2Cr2O7), cobalt chloride (CoCl2), ethylmethanesulfonate (EMS), and N-ethyl-N-nitrosourea (ENU) were clearly genotoxic. CPO alone at doses from 0.1 to 0.5% in Tween 80 was not mutagenic and did not enhance the mutagenic effect of the reference mutagens. However, antigenotoxic effects of CPO were clearly demonstrated in chronic cotreatments with mutagens and oil, by a significant decrease in wing spots induced by all four mutagens. The D. melanogaster wing spot test was found to be a suitable assay for detecting antigenotoxic effects in vivo.

The Potential of Orange Peel Oil as a Suppressor of Cell Proliferation in Animal Feed and Human Nutrition: An Experimental Study

“…Citrus avonoids have antioxidant, anticancer (Lai et al, 2013, Im et al, 2014, anti-mutagenic (Hosseinimehr and Karami, 2005;Demir et al, 2009), anti-allergic, antiinflammatory, and antimicrobial (Hamdan et al, 2013) properties. Additionally, citrus shells were used as a source of animal feed, fiber production, and fuel production (Bampidis and Robinson, 2006;Lashkari and Taghizadeh, 2013).…”

Human Hazard Assessment Using Drosophila Wing Spot Test as an Alternative In Vivo Model for Genotoxicity Testing—A Review

“…Buddleja globosa leaf extract might directly interact with ethyl methanesulfonate (EMS), an ethylating agent causing DNA mutation by transferring alkyl group to nucleotides, thereby inhibiting the alkylation process [121]. Citrus aurentium peel oil Yes 0.1-0.5% v/v of Citrus aurentium fruit peel oil inhibits wing spot formation induced by 0.5 mM EMS [126] Cryptocarya alba leaf extract Yes 4.74-9.79 mg/mL of Cryptocarya alba inhibits wing spot formation induced by 0.12 mg/mL EMS [34] Peumus boldus leaf extract Yes 2.28-9.12 mg/mL of Peumus boldus extract inhibits wing spot formation induced by 0.12 mg/mL EMS [ Ficus dubia root extract Weak 0.25, 1, and 2 mg/mL of Ficus dubia root extract weakly inhibits wing spot formation induced by 20 mM URE [133] Benzo[a]pyrene (BaP) is a well-known promutagen presented in the environment and in grilled or smoked food. BaP metabolizes into benzo(a)pyrene-7,8-diol 9,10-epoxide (BPDE), derived from phase I and Phase II enzymes that arrest and impede the progress of the DNA replication fork [134] and are highly mutagenic and carcinogenic [135].…”

Trifluralin,Treflan ve Etil Metan Sülfonatın Oreochromis niloticus’ta Oluşturduğu Genotoksik Hasar Üzerine Askorbik Asitin Antigenotoksik Etkisi

“…Balıklarda mikronukleus ve nuklear anomali testi, sucul çevrede hem ksenobiyotiklerin genotoksik etkilerinin değerlendirilmesinde hem de çeşitli kimyasalların antigenotoksik potansiyellerinin değerlendirilmesinde yoğun olarak kullanılmaktadır [22,23,24,25]. Bir kaç antioksidanın farklı kimyasalların genotoksisitesini azaltıp azaltmadığına dair çalışmalar literatürde mevcuttur [26,27,28].…”