Antigenotoxic effects of a polyherbal drug septilin against the genotoxicity of cyclophosphamide in mice

Sd, Shruthi; Vijayalaxmi, K.K.

doi:10.1016/j.toxrep.2016.07.001

Cited by 21 publications

(13 citation statements)

References 57 publications

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“…The micronuclei (MN) assay is a minimally invasive method as an evidence of chromosomal damage (Arul et al, 2017). It is used to detect the genotoxic effects of environmental mutagens (Shruthi and Vijayalaxmi, 2016). The incidence of micronucleated polychromatic erythrocytes (MnPCEs), caused appearance of some changes in polymorphism band patterns, and induced different histopathological lesions in the livers, kidneys, and testis (Khalil et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

The Potential Protective Role of Vitamin E and Selenium against Sub-chronic Toxicity of Hexavalent Chromium on the Testis of Adult Male Albino Rats

Mohammed

Abdelwahab

2020

Ain Shams Journal of Forensic Medicine and Clinical Toxicology

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Introduction Uptake of Hexavalent chromium (Cr 6) by the testis and its subsequent reduction to trivalent chromium is well known. Several studies attributed Cr 6 testicular toxicity to oxidative stress. Vitamin E is a lipid soluble antioxidant prevents damage to membranes. Selenium (Se) is an essential micronutrient with an antioxidant activity. This study aimed to investigate the potential protective role of vitamin E and selenium against sub-chronic toxicity of Cr 6 on testes of adult male albino rats through biochemical, cytogenetic and sperm analysis. Methodology: The duration of the present study was six weeks and was carried out on 60 adult male albino rats. They were divided into 10 rats of six groups, control group, selenium group (0.5 mg/kg by oral gavage), vitamin E group (125 mg/kg by oral gavage), Cr 6 group (10 mg/kg by oral gavage), Cr 6 + Selenium group and Cr 6 + Vitamin E group. Biochemical, cytogenetic and sperm analysis were done to all groups. Results: Levels of cholesterol, triglyceride, micronucleus polychromatic erythrocyte, total sperm abnormality are increased while glucose, serum glutathione-s-transferase, testosterone, DNA and RNA contents, sperm viability and content decreased. Treatment with Se or vit.E improved all these effects. Conclusion: Hexavalent chromium induced changes in biochemical, cytogenetic and sperm analysis, which lead to disruption in reproductive cell functions. Se or vit. E has the ability for reduction of these deleterious effects. Recommendation: Since vitamin E and selenium have antioxidant properties and play vital role in preventing hexavalent chromium induced toxicity, dietary supplementation with them is recommended for exposed workers and general population.

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Section: Discussionmentioning

confidence: 99%

The Potential Protective Role of Vitamin E and Selenium against Sub-chronic Toxicity of Hexavalent Chromium on the Testis of Adult Male Albino Rats

Mohammed

Abdelwahab

2020

Ain Shams Journal of Forensic Medicine and Clinical Toxicology

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“…The P/N ratio in normal condition is found to be 1 and suppression in its value suggests the cytotoxic effect of the compound [ 16 , 17 ]. The reduction in the total sperm count and increase in sperm shape abnormalities is reported to indicate defective spermatogenesis and structural damage to spermatozoa, respectively, and both are indicative of the nuclear abnormalities the germinal cell has undergone [ 5 , 18 ]. The diminished sperm motility has been linked to the ability of the compound to affect the viability of the sperm cells [ 19 ].…”

Section: Discussionmentioning

confidence: 99%

Morus alba Prevented the Cyclophosphamide Induced Somatic and Germinal Cell Damage in Male Rats by Ameliorating the Antioxidant Enzyme Levels

Ghosh

Rabbani

Asdaq³

et al. 2021

Molecules

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Cytogenetic analysis is essential to determine the effect of mutagens and antimutagens on genetic material. This study was done to evaluate the protective effect of root bark extract of Morus alba (M. alba) against cyclophosphamide induced somatic and germinal cell damage in male rats. The ethanolic extract of M. alba (0.25, 0.5 and 1 g/kg, 2 weeks) was evaluated against cyclophosphamide (75 mg/kg, single dose) induced nuclear damage. The sampling was done after 48 h of the clastogen treatment. The somatic and germinal nuclear damage was studied by bone marrow micronucleus and sperm analysis, respectively. Serum superoxide and catalase levels were estimated to determine the antioxidant status in each group. The results were analyzed statistically to find the significant variation. The administration of M. alba for 2 weeks suppressed dose-dependently the changes induced by cyclophosphamide. M. alba (0.5 g/kg) decreased the frequency of micronucleated erythrocyte, sperm shape abnormality and enhanced the sperm count, sperm motility and polychromatic-normochromatic erythrocytes ratio significantly (p < 0.05) in comparison with the cyclophosphamide treated group. The highest tested dose of M. alba (1 g/kg) produced more prominent suppression (p < 0.01) in the cyclophosphamide-induced somatic and germinal cell defects. The results also showed significant (p < 0.05) improvement in the serum antioxidant enzymes levels with M. alba when compared with the challenge group. The lower dose of M. alba extract (0.25 g/kg) prevented the CP-induced changes but was found to be statistically insignificant. Therefore, antimutagenic potential of the high dose of the extract of M. alba is possibly due to its antioxidant nature. The ability of the M. alba extract to prevent the nuclear damage could play an important role in overcoming several mutational defects that are associated with anticancer chemotherapy.

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“…It is well known that the antioxidant enzymes present in plants are responsible for their antigenotoxic activity. A majority of the phytochemicals with antioxidant activity also show anti-mutagenic and anti-genotoxic potential [51, 52, 53]. Therefore, the investigation on the antioxidant properties of ZFE was of prime importance.…”

Section: Discussionmentioning

confidence: 99%

Potential antigenotoxicity assessment of Ziziphus jujuba fruit

Goswami¹,

Banerjee²,

Mukherjee

2019

Heliyon

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Ziziphus jujuba Mill. fruits are nutritionally rich and have a broad spectrum of health benefits. In this work we hypothesized that this natural product rich in polyphenols might protect humans against DNA damage and its consequences. This has led to our investigation to find out if the fruit extract showed an ability to decrease the frequency of DNA damage (antigenotoxicity) induced by two known genotoxins namely an alkylating agent methyl methane sulphonate (MMS) and a reactive oxygen species (ROS) inducer hydrogen peroxide (H 2 O 2 ). Human lymphocytes were incubated with the Ziziphus fruit ethanol extracts (ZFE) or betulinic acid (BA) followed by an exposure to either 50 μM of MMS or 250 μM of H 2 O 2 . Results suggest that ZFE (250, 500, 1000 μg/ml) and BA (10, 20, 40 μg/ml) were able to inhibit the DNA damaging effect caused by MMS and H 2 O 2 indicative of their protection against the genotoxin. This could be attributed to the interactions of the phenolics, flavonoid and BA present in the fruits. Additional in vivo experiments were carried since BA is an important phytochemical detected in ample amounts in the fruit extract. Mice were primed with BA (2.5, 5.0 and 10 mg/kg body weight) for a period of 6 days. The animals were injected with MMS (10 mg/kg body weight) 24 h later and sacrificed. The genotoxic activity of MMS was inhibited in a dose – related manner by BA. BA reduced the frequency of MMS – induced DNA damage in liver, kidney and bone marrow cells of mice thereby exhibiting its antigenotoxic properties. It could also reduce total glutathione level, lipid peroxidation and hydrogen peroxide content in liver cells of mice through the up-regulation of antioxidant enzymes. Therefore taking into account the antioxidant and antigenotoxic properties, the consumption of the Ziziphus fruit should be more popularized worldwide.

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Antigenotoxic effects of a polyherbal drug septilin against the genotoxicity of cyclophosphamide in mice

Cited by 21 publications

References 57 publications

The Potential Protective Role of Vitamin E and Selenium against Sub-chronic Toxicity of Hexavalent Chromium on the Testis of Adult Male Albino Rats

The Potential Protective Role of Vitamin E and Selenium against Sub-chronic Toxicity of Hexavalent Chromium on the Testis of Adult Male Albino Rats

Morus alba Prevented the Cyclophosphamide Induced Somatic and Germinal Cell Damage in Male Rats by Ameliorating the Antioxidant Enzyme Levels

Potential antigenotoxicity assessment of Ziziphus jujuba fruit

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