Antigenemia and Antibody Titers to Core and Envelope Antigens in AIDS, AIDS-Related Complex, and Subclinical Human Immunodeficiency Virus Infection

Goudsmit, Jaap; Lange, Joep M. A.; Paul, Deborah A.; Dawson, George J.

doi:10.1093/infdis/155.3.558

Cited by 165 publications

(67 citation statements)

References 7 publications

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“…Otherwise, neutralizing antibodies and perhaps yet undefined factors in the plasma of some infected individuals inactivate HIV (892). Other procedures for increasing the detection of HIV in blood have included the measurement of p25 antigen (391) and HIV RNA by polymerase chain reaction (PCR) (480). However, the relation of these data to infectious particles, the potential source of transmission, is not clear.…”

Section: Hiv In Body Fluids and Its Reiation To Virus Transmissionmentioning

confidence: 99%

Pathogenesis of Human Immunodeficiency Virus Infection

Levy¹

1989

Annals of the New York Academy of Sciences

222

290

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Section: Hiv In Body Fluids and Its Reiation To Virus Transmissionmentioning

confidence: 99%

Pathogenesis of Human Immunodeficiency Virus Infection

Levy¹

1989

Annals of the New York Academy of Sciences

222

290

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“…And, it may depend on many factors, including T-helper lymphocyte counts, T-suppressor cell counts, the presence or absence of HIV antigen, and the presence or absence of HIV antibody (30,31,47,62,84,89).…”

Section: Properties Of Heterosexual Transmissionmentioning

confidence: 99%

Heterosexual Transmission of HIV: The Role of Other Sexually Transmitted Infections and Behavior in Its Epidemiology Prevention and Control

Aral¹

1993

Annu. Rev. Public Health

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“…The test cannot detect these antigen-antibody complexes. Even with a complex dissociation technique, the sensitivity remains low and can only detect about 50% of asymptomatic patients (6). Following this, a p24 antigen detection method modified with a booster step was developed by introducing a signal amplification boosted system to overcome the problem (2,8,9,13,14).…”

mentioning

confidence: 99%

p24 Antigen Detection Assay Modified with a Booster Step for Diagnosis and Monitoring of Human Immunodeficiency Virus Type 1 Infection

Sutthent¹,

Gaudart²,

Chokpaibulkit

et al. 2003

J Clin Microbiol

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We modified a p24 antigen enzyme-linked immunosorbent assay as a method for diagnosis and monitoring of human immunodeficiency virus type 1 (HIV-1) subtype E infection. This modified assay is based on the use of preheated immune complex dissociation combined with a booster step using a regular Vironostika HIV-1 p24 antigen assay (bioMerieux) to decrease the lower limit of p24 antigen detection from 10 pg/ml (lower limit achievable when using a regular p24 antigen assay) to 0.5 pg/ml (100 virions/ml) by the new method. The correlation between the values obtained by the HIV-1 RNA (Amplicor HIV-1 Monitor) assay and the p24 antigen assay modified with a booster step antigen assay in 160 frozen plasma samples with known viral load and 80 blind fresh plasma samples by Spearman rank were 0.671 (R 2 ‫؍‬ 0.450; P < 0.01) and 0.782 (R 2 ‫؍‬ 0.612; P < 0.01). During antiretroviral treatment, the change of p24 antigen level at >0.5 log correlated well with the level of HIV-1 in plasma. In order to improve the early diagnosis of HIV-1 infection in 121 infants born to HIV-1-infected mothers, a heat-denatured plasma p24 antigen assay modified with a booster step was compared with DNA-PCR and HIV RNA (nucleic acid sequence-based amplification) assays. The sensitivity of the antigen test modified with a booster step was similar to that of the HIV-1 RNA (NASBA QL) assay and better than that of the DNA-PCR assay (100 versus 61.90%) for subjects 1 to 2 months old. The overall results from this study might renew interest in p24 antigen detection as an easily affordable alternative method for diagnosis of HIV-1 infection and monitoring of disease progression in developing countries.

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Antigenemia and Antibody Titers to Core and Envelope Antigens in AIDS, AIDS-Related Complex, and Subclinical Human Immunodeficiency Virus Infection

Cited by 165 publications

References 7 publications

Pathogenesis of Human Immunodeficiency Virus Infection

Pathogenesis of Human Immunodeficiency Virus Infection

Heterosexual Transmission of HIV: The Role of Other Sexually Transmitted Infections and Behavior in Its Epidemiology Prevention and Control

p24 Antigen Detection Assay Modified with a Booster Step for Diagnosis and Monitoring of Human Immunodeficiency Virus Type 1 Infection

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