Antifungal and enzymatic evaluation of plant crude extracts derived from cinnamon and rosemary against Sclerotinia carrot rot

Ojaghian, Mohammad Reza; Chen, Yuzheng; Chen, S.; Cui, Zhou; Xie, Guoxin; Zhang, Jianhua

doi:10.1111/aab.12111

Cited by 30 publications

(18 citation statements)

References 55 publications

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“…In previous studies, crude extracts of some medicinal plants as well as cinnamaldehyde significantly increased the level of PPO, POD and PAL against storage carrot white mould. Moreover, sclerotia germination was markedly reduced due to the inhibitory effect of plants extract (Ojaghian, Chen, et al, ; Ojaghian, Wang, et al, , ). In conclusion, this study revealed that thyme and savoury EOs had potential to be used as resistance inducer against carrot white mould.…”

Section: Discussionmentioning

confidence: 99%

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Inhibitory efficacy of different essential oils against storage carrot rot with antifungal and resistance‐inducing potential

Ojaghian

Xie

Zhang

2019

Journal of Phytopathology

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The inhibitory effects of essential oils (EOs) derived from coriander, lavender, geranium, thyme, savoury and eucalyptus were assessed against Sclerotinia sclerotiorum, the causal agent of carrot white mould. All EOs showed antifungal activity against the pathogen in vitro and in vivo. In addition, all EOs markedly increased 6‐methoxymellein in the treated carrots. The EOs of thyme and savoury were found to be more effective than other tested EOs. The enzymatic tests showed that thyme and savoury EOs were more able than lavender EO to significantly increase the level of chitinases, peroxidases, β‐1,3‐glucanases, polyphenol oxidase, peroxidase and phenylalanine ammonia‐lyase in the treated carrots, indicating that the EOs of thyme and savoury have potential to be considered as effective inducers of resistance against carrot white mould. In this study, all EOs inhibited myceliogenic and carpogenic germination of sclerotia at concentrations 1 and 10 µl/ml sterile distilled water, respectively. After gas chromatography/mass spectrometry, major component in the thyme EO was found to be thymol (39.15%) followed by p‐cymene (13.85%) and carvacrol (10.36%), while in savoury EO were carvacrol (41.9%), γ‐terpinene (17.38) and p‐cymene (11.25%).

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Section: Discussionmentioning

confidence: 99%

“…The plates were remained at 22°C. After 20 days, per cent inhibition of sclerotial viability was measured using the method and formula described by Ojaghian, Chen, et al (2014b).…”

Section: Myceliogenic Germinationmentioning

confidence: 99%

Inhibitory efficacy of different essential oils against storage carrot rot with antifungal and resistance‐inducing potential

Ojaghian

Xie

Zhang

2019

Journal of Phytopathology

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“…The S. sclerotiorum is a ubiquitous phytopathogen which infects many dicotyledonous plants including Brassica crops. Although many research have been conducted to assess control measures against Sclerotinia diseases on different crops, [4][5][6][7][8][9][10] no research has been found about nonchemical methods against tuber mustard white rot. The in vitro antifungal effect of tested chitosans and ASA was evaluated in previous research.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, the percent inhibition of sclerotia formation was calculated after 15 days according to the formula S=100-(Ts×100/Cs), where: S= percent inhibition of sclerotia formation, Ts= the number of sclerotia in treatment and Cs= the number of sclerotia in control. 8 This experiment was repeated 3 times with 5 repetitions for each treatment, and the effects of different treatments were determined in a completely randomized design.…”

Section: In Vitro Effect Of Chitosans Against Sclerotinia Sclerotiorummentioning

confidence: 99%

“…Even though no study has been carried out to evaluate yield loss caused by S. sclerotiorum in Hangzhou, Sclerotinia rot of mustard is reported to cause yield losses up to 40 percent in India. 2 Although some control methods such as biological control, 3-5 biofumigation 6,7 and application of plant crude extracts 8,9 have been effective against Sclerotinia diseases, the majority of local mustard growers prefer the application of fungicides mostly iprodione and dichloran. 10 However, due to increasing concerns about the environment, human health and the development of fungicide resistance several research have been conducted in the Zhejiang province to introduce sustainable control methods to farmers in order to reduce fungicides applied in mustard fields.…”

Section: Introductionmentioning

confidence: 99%

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Resistance Induction Against White Rot of Tuber Mustard Using Chitosans and Acetyl Salicylic Acid

Ojaghian

Xie

2019

J Apple Biotechnol Rep

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Introduction: White rot, caused by Sclerotinia sclerotiorum, has recently become a serious threat to tuber mustard cultivation in Hangzhou, China. The objective of this study was to evaluate the inhibitory effect of acetyl salicylic acid (ASA) and three different chitosans (A, B and C) against mustard white rot. The degree of N-deacetylation and the molecular weight of chitosans A, B and C were 85%-1129 kDa, 95%-521 kDa and 75%-607 kDa, respectively. Materials and Methods: The inhibitory effect of chitosans with different concentrations against the mycelia growth and sclerotia formation of 3 isolates of the pathogen was determined in vitro. In addition, the efficacy of chitosans and ASA against mustard white rot was assessed during in vivo tests. After protein extraction, effects of chitosans and ASA on resistance related enzymes including chitinase, β-1,3-glucanase, phenylalanine ammonia lyase, polyphenol oxidase (PPO) and peroxidase (POD) were evaluated. Results: The chitosans reduced the mycelia growth and sclerotia formation of the pathogen. The chitosans showed significant antifungal effect against the disease in vivo. The chitosans and ASA markedly reduced the severity of the disease over time. Moreover, the chitosans and ASA markedly enhanced the level of most of the resistant related enzymes after 3 and 6 days. The chitosan B was found to have the best effect against tested pathogen isolates. Conclusions: The chitosan with the lowest molecular weight was found to be more effective against the disease. In addition, chitosans and ASA were able to significantly increase resistance-related enzymes over time indicating that they can be considered as resistant inducers against mustard white rot.

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Cinnamon powder: an in vitro and in vivo evaluation of antifungal and plant growth promoting activity

et al. 2019

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In this study, the effects of organic powder of Cinnamomum zeylanicum on the development of Botrytis cinerea and its influence on tomato plants were evaluated. The cinnamon bark powder and its water suspensions and filtrates were used at 0.5 and 1% rates. After 6 days of the start of an in vitro experiment the mycelium growth was inhibited by both 0.5 and 1% cinnamon water filtrates-to a greater degree in the case of the higher concentration, by 54.4 and 81.4%, respectively. Spraying with cinnamon water filtrates positively influenced the growth of plants both in the greenhouse and the field. Antifungal activity of cinnamon was proved in the greenhouse tests-the disease symptoms of grey mould on infected tomato plants decreased. The fresh weight of non-inoculated tomato plants treated with cinnamon filtrates was significantly higher than control plants (17.17 g compared to 12.83 g) showing a stimulating effect of cinnamon filtrates. In the case of inoculated plants due to treatment, their weight increased from 7.83 to 10.50 g. In the field experiment, tomato plants sprayed six times with cinnamon were better developed than the control plants. The most significant effect was observed for Hamlet variety-the mean number of leaves was higher by 27.3% and the mean number of branches by 19.7% compared to the untreated control plants. Thus it was proved that cinnamon powder has potential to inhibit B. cinerea growth and also has a stimulating effect for tomato plants.

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Antifungal and enzymatic evaluation of plant crude extracts derived from cinnamon and rosemary against Sclerotinia carrot rot

Cited by 30 publications

References 55 publications

Inhibitory efficacy of different essential oils against storage carrot rot with antifungal and resistance‐inducing potential

Inhibitory efficacy of different essential oils against storage carrot rot with antifungal and resistance‐inducing potential

Resistance Induction Against White Rot of Tuber Mustard Using Chitosans and Acetyl Salicylic Acid

Cinnamon powder: an in vitro and in vivo evaluation of antifungal and plant growth promoting activity

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