Antifreeze Glycoproteins from Antarctic Notothenioid Fishes Fail to Protect the Rat Cardiac Explant during Hypothermic and Freezing Preservation

Wang, Ting‐Chung; Zhu, Qingyan; Yang, Xiaoping; Layne, Jack R.; DeVries, Arthur L.

doi:10.1006/cryo.1994.1022

Cited by 67 publications

(53 citation statements)

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“…Furthermore, to the best of our knowledge, there are only three reported syntheses of AF(G)Ps because of the difficulty of installing the correct disaccharide moiety, and have only succeeded on a small scale [12][13][14] . The second problem is that the majority of attempts at cryopreservation using AF(G)Ps and AFPs have shown minimal benefit, and in most cases a decrease in cell recovery has been observed 11,15,16 . This has been attributed to a secondary property of AF(G)Ps known as dynamic ice shaping (DIS), which is intrinsically linked to their TH property.…”

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confidence: 99%

“…Carpenter et al 11 and Matsumoto et al 18 demonstrated small improvements in cryopreservation with an AFP and an AF(G)P mimic respectively, but the benefit was limited by the low concentrations, which could be applied before cell viability was reduced because of ice-shaping. Wang et al 15 observed that AF(G)P failed to preserve rat hearts. Bovine sperm and mouse oocytes 19 appear to benefit from AFP during cryopreservation but AFPs reduce the viability of mouse sperm under similar conditions 16 .…”

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Synthetic polymers enable non-vitreous cellular cryopreservation by reducing ice crystal growth during thawing

et al. 2014

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The cryopreservation of cells, tissue and organs is fundamental to modern biotechnology, transplantation medicine and chemical biology. The current state-of-the-art method of cryopreservation is the addition of large amounts of organic solvents such as glycerol or dimethyl sulfoxide, to promote vitrification and prevent ice formation. Here we employ a synthetic, biomimetic, polymer, which is capable of slowing the growth of ice crystals in a manner similar to antifreeze (glyco)proteins to enhance the cryopreservation of sheep and human red blood cells. We find that only 0.1 wt% of the polymer is required to attain significant cell recovery post freezing, compared with over 20 wt% required for solvent-based strategies. These results demonstrate that synthetic antifreeze (glyco)protein mimics could have a crucial role in modern regenerative medicine to improve the storage and distribution of biological material for transplantation.

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Synthetic polymers enable non-vitreous cellular cryopreservation by reducing ice crystal growth during thawing

et al. 2014

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“…This spiculated ice growth and the resulting cellular damage that ensues has been extensively documented [10,12,67,79]. It is interesting however that this effect is negated when higher concentrations of DMSO are utilized.…”

Section: (A) 0% Dmso + 1 Mg/ml Af(g)p (B) 2% Dmso + 1 Mg/ml Af(g)pmentioning

confidence: 85%

“…Consequently, AF(G)Ps often fail to effectively protect mammalian cells from cryoinjury. This is due in part to non-specific toxic effects and the change in ice crystal habit caused by the irreversible binding of the AF(G)P to ice [10,12,67,79]. Thus, the rational design of ice recrystallization inhibitors lacking TH activity (and the ability to bind to ice) is a worthwhile goal [4,8,[30][31][32]63].…”

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Modulation of antifreeze activity and the effect upon post-thaw HepG2 cell viability after cryopreservation

et al. 2015

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“…Spicular ice formed rapidly in the presence of 10 mg/ml AFGP and needle-like crystals appeared to penetrate the cardiomyocytes, resulting in intracellular freezing followed by cell lysis. Wang et al (1994) evaluated subzero cryopreservation of rat hearts using the Langendorff in vitro model of working isolated rat hearts. Cardiac explants were preserved using AFGP at different concentrations at subzero temperatures of −1.4°C.…”

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Lessons from nature for preservation of mammalian cells, tissues, and organs

Brockbank

Campbell

Greene

et al. 2010

In Vitro Cell.Dev.Biol.-Animal

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The study of mechanisms by which animals tolerate environmental extremes may provide strategies for preservation of living mammalian materials. Animals employ a variety of compounds to enhance their survival, including production of disaccharides, glycerol, and antifreeze compounds. The cryoprotectant glycerol was discovered before its role in amphibian survival. In the last decade, trehalose has made an impact on freezing and drying methods for mammalian cells. Investigation of disaccharides was stimulated by the variety of organisms that tolerate dehydration stress by accumulation of disaccharides. Several methods have been developed for the loading of trehalose into mammalian cells, including inducing membrane lipid-phase transitions, genetically engineered pores, endocytosis, and prolonged cell culture with trehalose. In contrast, the many antifreeze proteins (AFPs) identified in a variety of organisms have had little impact. The first AFPs to be discovered were found in cold water fish; their AFPs have not found a medical application. Insect AFPs function by similar mechanisms, but they are more active and recombinant AFPs may offer the best opportunity for success in medical applications. For example, in contrast to fish AFPs, transgenic organisms expressing insect AFPs exhibit reduced ice nucleation. However, we must remember that nature's survival strategies may include production of AFPs, antifreeze glycolipids, ice nucleators, polyols, disaccharides, depletion of ice nucleators, and partial desiccation in synchrony with the onset of winter. We anticipate that it is only by combining several natural low temperature survival strategies that the full potential benefits for mammalian cell survival and medical applications can be achieved.

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Antifreeze Glycoproteins from Antarctic Notothenioid Fishes Fail to Protect the Rat Cardiac Explant during Hypothermic and Freezing Preservation

Cited by 67 publications

References 0 publications

Synthetic polymers enable non-vitreous cellular cryopreservation by reducing ice crystal growth during thawing

Synthetic polymers enable non-vitreous cellular cryopreservation by reducing ice crystal growth during thawing

Modulation of antifreeze activity and the effect upon post-thaw HepG2 cell viability after cryopreservation

Lessons from nature for preservation of mammalian cells, tissues, and organs

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