Antifeedant activity of ethanolic extract from Flourensia oolepis and isolation of pinocembrin as its active principle compound

Napal, Georgina Natalia Díaz; Carpinella, María Cecilia; Palacios, Sara M.

doi:10.1016/j.biortech.2009.02.050

Cited by 78 publications

(42 citation statements)

References 42 publications

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“…Other resin components in Flourensia spp. have also been reported to have insect antifeedant [20]- [22], antifungal [23], antibacterial [24] [25], antialgal and antitermite properties [26], and herbicidal [10] [15] [20], and to act as tyrosinase inhibitors [27]. To our knowledge, the link between resins secretion and secretory structures in the genus Flourensia has never been addressed before.…”

Section: Introductionmentioning

confidence: 97%

Secretory Structures in Flourensia campestris and F. oolepis: Ultrastructure, Distribution, and (-)-Hamanasic Acid A Secretion

Silva¹,

Tourn²,

López³

et al. 2015

AJPS

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In this work, the localization, density, morphology and ultrastructure of secretory structures in aerial organs of Flourensia campestris (FC) and F. oolepis (FO) (Asteraceae) by means of a combination of light, fluorescence, transmission (TEM) and scanning electron microscopy (SEM) were examined. The possible role of secretory structures in the production and secretion of the phytotoxic sesquiterpene (-)-hamanasic acid A ((-)HAA) in both species was also assessed. Capitate glandular trichomes were found in all reproductive organs of FC and FO, and were being reported for the first time. These glandular trichomes, typically associated to edges and veins, were of the same type as those already described for vegetative organs, and were abundant in involucral bracts and corolla of tubulose and ligulate flowers. Their density in reproductive organs of both species was similar (ca. 30/mm 2 ) and lower than that found in leaves (ca. 100/mm 2 ) and stems (ca. 160/mm 2 in FC, and up to 650/mm 2 in FO). Glandular trichomes in vegetative organs followed a species-* Corresponding authors. M. P. Silva et al. 926specific pattern of distribution. TEM and SEM observations suggest that each species differs in the way in which secretory materials are released to the outside: through cracks or pores in FC, or through a loose cuticle in FO. Similar inspections of the secretory ducts revealed lipophilic vacuoles localized in subepithelial and epithelial cells, in which secretions accumulated before being transferred to the duct. The presence of wall ingrowths in subepithelial cells suggests that granulocrine secretion operates in these species. Secretory ducts varied in density and diameter among the organs in both species, with the combination being maximal in woody stems. (-)HAA was only detected in surface secreted resins of both species, and its concentration (2D-TLC, GC-FID) was intimately associated with the distribution and density of glandular trichomes in each organ (capitula, leaves, and stems with primary or secondary growth). In addition, no (-)HAA was detected internally in the resins collected from secretory ducts. The composition of these resins showed distinctive profiles for FC and FO, and only four from ca. 30 compounds detected (GC/MS) were shared by both species. In addition to the elucidation of ultrastructural traits, distribution and density of secretory structures in aerial organs of FC and FO, present findings suggest a functional role for glandular trichomes in the secretion of the putative phytotoxic allelochemical (-)HAA.

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Section: Introductionmentioning

confidence: 97%

Secretory Structures in Flourensia campestris and F. oolepis: Ultrastructure, Distribution, and (-)-Hamanasic Acid A Secretion

Silva¹,

Tourn²,

López³

et al. 2015

AJPS

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“…These are synthesized by the phenylpropanoid pathway (Ververidis et al, 2007) and impart pigmentation to flowers (Tanaka et al, 2008), provide protection against damage by UV radiation (Izaguirre et al, 2007) and insects (Thoison et al, 2004;Diaz Napal et al, 2009), as well as act as signaling molecules (Kobayashi et al, 2004). About 20% of the carbon fixed in photosynthesis is directed into this pathway, which produces the majority of phenolic compounds found in nature.…”

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confidence: 99%

Modulation of Transcriptome and Metabolome of Tobacco by Arabidopsis Transcription Factor, AtMYB12, Leads to Insect Resistance

et al. 2010

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Flavonoids synthesized by the phenylpropanoid pathway participate in myriad physiological and biochemical processes in plants. Due to the diversity of secondary transformations and the complexity of the regulation of branched pathways, single gene strategies have not been very successful in enhancing the accumulation of targeted molecules. We have expressed an Arabidopsis (Arabidopsis thaliana) transcription factor, AtMYB12, in tobacco (Nicotiana tabacum), which resulted in enhanced expression of genes involved in the phenylpropanoid pathway, leading to severalfold higher accumulation of flavonols. Global gene expression and limited metabolite profiling of leaves in the transgenic lines of tobacco revealed that AtMYB12 regulated a number of pathways, leading to flux availability for the phenylpropanoid pathway in general and flavonol biosynthesis in particular. The tobacco transgenic lines developed resistance against the insect pests Spodoptera litura and Helicoverpa armigera due to enhanced accumulation of rutin. Suppression of flavonol biosynthesis by artificial microRNA reversed insect resistance of the AtMYB12-expressing tobacco plants. Our study suggests that AtMYB12 can be strategically used for developing safer insect pest-resistant transgenic plants.

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“…This consideration suggests that the above compounds may play different ecophysiological functions during the plant development. As a matter of fact it has been shown that pinocembrin (1) isolated from Flourencia oolepis demonstrates a strong antifeedant activity against Epilachna paenulata, Xanthogaleruca luteola and Spodoptera frugiperda 25 . In addition, the pinocembrin (1) action mechanism in Epilachna paenulata is chronic intoxication, rather than simple starvation from antifeedant effects 26 .…”

Section: Resultsmentioning

confidence: 99%

Seasonal Variation of the Flavonoids Pinocembrin and 3-O-Methylgalangin, in the Surface Component Mixture (Resinous Exudates and Waxy Coating) of Heliotropium Stenophyllum

Modak

Torres

Urzúa

2011

J. Chil. Chem. Soc.

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In this report we study the seasonal variation of the flavonoids pinocembrin and 3-O-methylgalangin in the surface component mixture (resinous exudate and waxy coating) of Heliotropium stenophyllum. The quantitative analysis of the flavonoids was performed using high-performance liquid chromatography of samples collected monthly over a whole year. The results showed an increase in the spring and summer yield of surface components and a decrease during the winter. Although the sum of pinocembrin and 3-O-methylgalangin did not follow a pattern related with hydric stress, UV radiation or high temperature during the year, a relationship between pinocembrin and 3-O-methylgalangin was found. On average during the months of September to August, excluding March, the amount of pinocembrin decreased wile the amount of 3-O-methylgalangin increased. The results suggest that the above compounds may play different ecophysiological functions during plant development and are consistent with the biosynthetic relationship between the two compounds.

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Antifeedant activity of ethanolic extract from Flourensia oolepis and isolation of pinocembrin as its active principle compound

Cited by 78 publications

References 42 publications

Secretory Structures in <i>Flourensia campestris</i> and <i>F. oolepis</i>: Ultrastructure, Distribution, and (-)-Hamanasic Acid A Secretion

Secretory Structures in <i>Flourensia campestris</i> and <i>F. oolepis</i>: Ultrastructure, Distribution, and (-)-Hamanasic Acid A Secretion

Modulation of Transcriptome and Metabolome of Tobacco by Arabidopsis Transcription Factor, AtMYB12, Leads to Insect Resistance

Seasonal Variation of the Flavonoids Pinocembrin and 3-O-Methylgalangin, in the Surface Component Mixture (Resinous Exudates and Waxy Coating) of Heliotropium Stenophyllum

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Antifeedant activity of ethanolic extract from Flourensia oolepis and isolation of pinocembrin as its active principle compound

Cited by 78 publications

References 42 publications

Secretory Structures in &lt;i&gt;Flourensia campestris&lt;/i&gt; and &lt;i&gt;F. oolepis&lt;/i&gt;: Ultrastructure, Distribution, and (-)-Hamanasic Acid A Secretion

Secretory Structures in &lt;i&gt;Flourensia campestris&lt;/i&gt; and &lt;i&gt;F. oolepis&lt;/i&gt;: Ultrastructure, Distribution, and (-)-Hamanasic Acid A Secretion

Modulation of Transcriptome and Metabolome of Tobacco by Arabidopsis Transcription Factor, AtMYB12, Leads to Insect Resistance

Seasonal Variation of the Flavonoids Pinocembrin and 3-O-Methylgalangin, in the Surface Component Mixture (Resinous Exudates and Waxy Coating) of Heliotropium Stenophyllum

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Secretory Structures in <i>Flourensia campestris</i> and <i>F. oolepis</i>: Ultrastructure, Distribution, and (-)-Hamanasic Acid A Secretion

Secretory Structures in <i>Flourensia campestris</i> and <i>F. oolepis</i>: Ultrastructure, Distribution, and (-)-Hamanasic Acid A Secretion