Anticoagulant and anti-platelet activity of polyphenolic-polysaccharide preparation isolated from the medicinal plant Erigeron canadensis L.

Pawlaczyk, Izabela; Czerchawski, L; Kuliczkowski, Wiktor; Karolko, Bożena; Pilecki, Witold; Witkiewicz, Wojciech; Gancarz, Roman

doi:10.1016/j.thromres.2010.11.031

Cited by 97 publications

(66 citation statements)

References 27 publications

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“…Thus in the present study, in vitro thrombolytic activity showed that B. orthobotrys aqueous methanolic extract possesses notable ability to break down blood clot thus strengthening (Manach et al, 2004). Formerly, the polysaccharide phenolic protein complex has been found to exhibit both anticoagulant and procoagulant effects on blood coagulation system (Pawlaczyk et al, 2011). Also, Pawlaczyk et al (2011) proved phenolic polysaccharide isolated from Erigeron canadensis as a potentially useful anticoagulant and antiplatelet agents.…”

Section: Discussionmentioning

confidence: 54%

“…Formerly, the polysaccharide phenolic protein complex has been found to exhibit both anticoagulant and procoagulant effects on blood coagulation system (Pawlaczyk et al, 2011). Also, Pawlaczyk et al (2011) proved phenolic polysaccharide isolated from Erigeron canadensis as a potentially useful anticoagulant and antiplatelet agents. HPLC screening of B. orthobotrys revealed the presence of quericitin, gallic acid, 4-hydroxy-3-methoxy benzoic acid and p-coumaric acid.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of anticoagulant and thrombolytic activity of Berberis orthobotrys in animal model

Alamgeer

Tarar

Hasan

et al. 2018

Bangladesh J Pharmacol

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Fundamental to this research was the evaluation of anticoagulant and thrombolytic potential of Berberis orthobotrys using clotting time, prothrombin time and activated partial thromboplastin time performed at 2.5, 5, 10% concentration. Aqueous-methanol extract significantly (p<0.05) increased the coagulation parameters both in human blood as well as in rabbit blood and exhibited the more salient effect on prolongation of these coagulation parameters at 10% concentration. Aqueous methanol extract (25, 50 and 100 mg/kg) produced the preferential increase in bleeding time and clotting time nevertheless the effect was more perceptible after 90 min at 100 mg/kg. Moreover, the crude extract presented a substantial increase in coagulation parameters after 7 days of administration in rabbits with more pronounced effects at the highest dose. HPLC profile of aqueous methanol extract revealed the presence of quercetin, p-coumaric acid, gallic acid, vanillic acid, 4-hydroxy-3-methoxy benzoic acid. Thus, the present study provides the pharmacological basis for its medical use in cardiovascular and other thrombotic disorders.

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Section: Discussionmentioning

confidence: 54%

Section: Discussionmentioning

confidence: 99%

Evaluation of anticoagulant and thrombolytic activity of Berberis orthobotrys in animal model

Alamgeer

Tarar

Hasan

et al. 2018

Bangladesh J Pharmacol

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“…Yield variations of polysaccharide extraction can be explained by the changes in their distribution in plant parts and also by the type of extraction employed (Gottlieb & Borin, 2004). EP-Mt revealed high content of carbohydrate (41%), which was superior in comparison with that obtained from C. ferrea (31%) (Pereira et al, 2012a) and Erigeron canadensis flowers (34.1%) (Pawlaczyk et al, 2011). Importantly, the protein content (0.34%) was much less compared to the polysaccharides of Geoffroea spinosa barks (9%) (Silva et al, 2015), C. ferrea (3%) (Pereira et al, 2012a) and A. indica (4%) (Pereira et al, 2012b), both extracted by the same protocol.…”

Section: Resultsmentioning

confidence: 67%

Polysaccharide extract of Mimosa tenuiflora stem barks stimulates acute inflammatory response via nitric oxide

Silva-Leite

Queiroz

Madeira

et al. 2016

Acta Sci. Biol. Sci.

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ABSTRACT. Mimosa tenuiflora (Mimosaceae) or "jurema-preta" is well distributed in the northeast Brazil, being popularly used to treat skin lesions, burns and inflammation. The healing effect of the alcoholic extract prepared with its barks corroborates the popular use. This study aimed to evaluate the inflammatory response of polysaccharides extracted from M. tenuiflora barks (EP-Mt) by methanol/NaOH and ethanol precipitation. Inflammatory activity was assessed in rat models of acute inflammation (paw edema and peritonitis), by the following parameters: edema, vascular permeability, leukocyte migration, myeloperoxidase activity and pharmacological modulation of nitric oxide and prostaglandins. EP-Mt presented 3.8% yield, 41% carbohydrate and 0.34% protein. EP-Mt (0.01, 0.1, 1.0 mg kg -1 ) injected by subcutaneous route elicited paw edema that lasted from 30-420 min, with maximal effect at 1 mg kg -1 (40x vs. saline), and was inhibited by L-NAME (52%) and dexamethasone (26%). EP-Mt (1 mg kg -1 , via intraperitoneal) stimulated leukocytes migration (2.2x), mainly neutrophils (6.5x) and MPO activity (96%). The leukocyte migration elicited by EP-Mt was inhibited by dexamethasone (39%) and L-NAME (38%). EP-Mt containing high carbohydrate content induces acute inflammation via nitric oxide, which open perspectives of application in pathological conditions of immunosuppression.Keywords: Mimosaceae, medicinal plant, carbohydrate, inflammation.Extrato polissacarídico oriundo das cascas do caule de Mimosa tenuiflora induz resposta inflamatória aguda via óxido nítrico RESUMO. Mimosa tenuiflora (Mimosaceae) ou "jurema-preta", amplamente distribuída no nordeste brasileiro, é utilizada popularmente no tratamento de lesões de pele, queimaduras e inflamação. O efeito cicatrizante do extrato alcoólico de suas cascas corrobora o uso popular. Avaliou-se o efeito inflamatório dos polissacarídeos da casca de M. tenuiflora (EP-Mt), obtidos por extração com metanol/NaOH e precipitação com etanol. O efeito inflamatório foi avaliado em modelos agudos em ratos (edema de pata, peritonite) por meio dos seguintes parâmetros: edema, permeabilidade vascular, migração leucocitária, atividade da mieloperoxidase e modulação farmacológica de prostaglandinas e óxido nítrico. EP-Mt apresentou 3,8% de rendimento, 41% de carboidratos totais e 0,34% de proteína. EP-Mt (0,01, 0,1, 1,0 mg kg -1 ), administrado por via subcutânea, induziu edema de pata com duração de 30 a 420 min e efeito máximo na dose de 1 mg kg -1 (40x vs. salina), o qual foi inibido por L-NAME (52%) e dexametasona (26%). EP-Mt (1 mg kg -1 , via intraperitoneal) estimulou a migração de leucócitos (2,2x vs. salina), principalmente de neutrófilos (6,5x), com aumento da atividade da mieloperoxidase (96%). A migração de leucócitos foi inibida por dexametasona (39%) e L-NAME (38%). EP-Mt contendo elevado teor de carboidratos induz inflamação aguda via óxido nítrico com perspectivas de aplicação em condições patológicas de imunossupressão.Palavras-chave: Mimosaceae, plantas medicinais, carb...

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“…The chemical analysis of most of the presented plant preparations was published recently [10,12]. The analyses of A. millefolium preparation as well of the C. recutita preparation were not published and were done using the same methods and their conditions.…”

Section: Chemical Analysismentioning

confidence: 99%

“…The polyphenolic-polysaccharide conjugates isolated from Conyza canadensis L. [10] as well as from the other medicinal plants of Asteraceae family, including Achillea millefolium L., Arnica montana L., Echinacea purpurea (L.) Moench, Solidago virgaurea L. and Chamomilla recutita (L.) Rauschert., had anticoagulant in vitro activity on human blood plasma [11,12]. However, the role of the polyphenolic-polysaccharide conjugates present in these medicinal plants selected from Asteraceae family in the defence against oxidative/nitrative stress in human platelets is still unknown.…”

Section: Introductionmentioning

confidence: 99%

The effect of polyphenolic-polysaccharide conjugates from selected medicinal plants of Asteraceae family on the peroxynitrite-induced changes in blood platelet proteins

Saluk-Juszczak

Pawlaczyk

Olas

et al. 2010

International Journal of Biological Macromolecules

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Anticoagulant and anti-platelet activity of polyphenolic-polysaccharide preparation isolated from the medicinal plant Erigeron canadensis L.

Cited by 97 publications

References 27 publications

Evaluation of anticoagulant and thrombolytic activity of Berberis orthobotrys in animal model

Evaluation of anticoagulant and thrombolytic activity of Berberis orthobotrys in animal model

<b>Polysaccharide extract of <i>Mimosa tenuiflora</i> stem barks stimulates acute inflammatory response via nitric oxide

The effect of polyphenolic-polysaccharide conjugates from selected medicinal plants of Asteraceae family on the peroxynitrite-induced changes in blood platelet proteins

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