Anticellular and immunosuppressive properties of ethanolic extract of Acorus calamus rhizome

Mehrotra, Shikhar; Mishra, K.P.; Maurya, Rakesh; Srimal, R. C.; Yadav, V. S.; Pandey, Rahul; Singh, Vijay K.

doi:10.1016/s1567-5769(02)00212-6

Cited by 101 publications

(42 citation statements)

References 25 publications

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“…The cell culture was performed in microtiter plates (TTP, Techno Plastic Products, Trasadingen, Switzerland) with incubation of the cells (50,000 cells/mL) and methanol extracts (10, 50, 100 and 200 µg/mL) in the presence or absence of PHA (Sigma, 5 µg/mL) for 72 hours at 37 °C and in 5% CO 2 (Jouan, model IG150, Saint Herbain, France) (Yasni et al, 1993;Roseghini et al, 2006). The cell and mitogen concentrations were determined by the growth curve (data not shown), while the concentrations of the extracts were defined according to the literature (Wilasrusmee et al, 2002;Manosroi, Saraphanchotiwitthaya, Manosroi, 2003;Mehrotra et al, 2003). The assay revelation was performed by blue tetrazolium reduction assay (MTT), with the addition of 10 µL of MTT at 5 mg/mL (Amreco, Solon, Ohio, USA) in 0.9% NaCl to each well of the microplate three hours before the end of the incubation period.…”

Section: Cell Culturementioning

confidence: 99%

Evaluation of the proliferative activity of methanol extracts from six medicinal plants in murine spleen cells

Zandonai

Coelho

Ferreira

et al. 2010

Braz. J. Pharm. Sci.

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A number of natural compounds have been used as immunomodulatory agents, enabling the function of the immune system to be modified by stimulating or suppressing it. There has been increasing interest in the study of therapeutic action of plant extracts regarding their immunomodulatory activity. The aim of this study was to identify and evaluate the action of extracts of the medicinal plants Calophyllum brasiliense, Ipomoea pes-caprae, Matayba elaeagnoides, Maytenus robusta, Rubus imperialis and Vernonia scorpioides on the development of spleen cells from mice, using the in vitro cellular proliferation assay. The cells, obtained by mechanical rupture of mice spleen (5x10 4 cells/mL), were incubated with methanol extracts (10, 50, 100 and 200 mg/mL) and phytohemagglutinin (PHA, 5 mg/mL). The basal control for proliferation consisted of cells alone, while the positive control consisted of cells and PHA. The cell culture was kept at 37 o C in 5% CO 2 for 72 hours, and cell proliferation was revealed by the blue tetrazolium reduction assay (MTT). The results were expressed as percentage of growth and were analyzed using the Kruskal-Wallis and Mann-Whitney tests. The C. brasiliense, I. pes-caprae and M. elaeagnoides extracts showed dose-dependent induction of cell proliferation, with a significant increase in cell proliferation (p<0.03) and percentage growth of 88.2%, 73.1% and 52.7%, respectively, suggesting T lymphocyte stimulation. By contrast, M. robusta, R. imperialis and V. scorpioides extracts showed significance only with a negative percentage of growth, suggesting inhibition of cell proliferation (p<0.04). Further biomonitoring studies will enable the fractions and isolated substances responsible for the immunomodulatory activities to be identified. Várias substâncias de origem natural têm sido utilizadas como agentes imunomoduladores, permitindo modificar a função do sistema imune e propiciando o estudo de atividades terapêuticas de extratos de plantas. Este trabalho objetivou identificar a atividade imunomodulatória dos extratos de seis plantas medicinais da flora brasileira, Calophyllum brasiliense, Ipomoea pes-caprae, Matayba elaeagnoides, Maytenus robusta, Rubus imperialis e Vernonia scorpioides, sobre a proliferação de células esplênicas de camundongos. As células esplênicas murinas obtidas por ruptura mecânica do baço (5x14 3 células/mL) foram incubadas com os extratos metanólicos das plantas (10, 50, 100, 200 mg/mL) e fito-hemaglutinina (PHA, 5 mg/mL). O controle basal de proliferação foi constituído de células apenas e o controle positivo formado por células e PHA. O cultivo celular foi mantido a 37 o C, 5% de CO 2 , 72 horas, com quantificação da proliferação celular pelo ensaio de redução do azul de tetrazólio. Os resultados expressos em percentagem de crescimento foram analisados pelos testes de Kruskal-Wallis e Mann-Whitney. Os extratos de C. brasiliense, I. pes-caprae e M. elaeagnoides mostraram indução dose-dependente da proliferação celular (p<0,03), com percentagem de crescimento de, respecti...

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Section: Cell Culturementioning

confidence: 99%

Evaluation of the proliferative activity of methanol extracts from six medicinal plants in murine spleen cells

Zandonai

Coelho

Ferreira

et al. 2010

Braz. J. Pharm. Sci.

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“…Essential oil, a-asarone, b-asarone Arora, 1965;Chopra et al, 1954;Madan et al, 1960;Mamgain & Singh, 1994;Moholkar et al, 1975 Hypolipidemic activity a-Asarone, 50% ethanol extract Garduno et al, 1997;Parab & Mengi, 2002;Mukherjee, 2003 Actions on respiratory system Alcohol extract Bose et al, 1960;Chandra, 1980;Rajasekharan & Srivastava, 1977 Anticancer activity Lectins, ethanol extract Bains et al, 2005;Mehrotra et al, 2003 Immunosuppressive activity Ethanol extract Mehrotra et al, 2003 Anti-inflammatory Coconut oil extract Varde et al, 1988;Vohora et al, 1989 Antiulcer and cytoprotective properties Ethanol extract Rafatullah et al, 1994 (Continued) Antispasmodic Essential oil, water-soluble fraction of dried alcohol extract, a-asarone Agarwal et al, 1956;Bhakuni et al, 1988;Bose et al, 1960;Chopra et al, 1954;Das et al, 1962;…”

Section: Cns Depressant Activitiesmentioning

confidence: 99%

Acorus calamus.: Scientific Validation of Ayurvedic Tradition from Natural Resources

Mukherjee

Venkatesan

Mal

et al. 2007

Pharmaceutical Biology

109

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Acorus calamus Linn. (Araceae), commonly known as ''sweet flag'' or ''calamus'', is a semiaquatic, perennial, aromatic herb with creeping rhizomes. The plant is found in the northern temperate and subtropical regions of Asia, North America, and Europe. The plant exhibits polyploidy. Many ethnomedicinal and ethnobotanical uses have been ascribed to the rhizomes of the plant. A. calamus Linn. (AC) has been used as traditional Chinese and Indian prescriptions for its beneficial effects on memory disorder, learning performance, lipid peroxide content, and anti-aging and anticholinergic activity. Moreover, pharmacological studies have revealed that Acorus rhizome and its constituents, particularly a-and b-asarone, possess a wide range of pharmacological activities such as sedative, CNS depressant, behavior modifying, anticonvulsant, acetylcholinesterase inhibitory, memory enhancing, anti-inflammatory, antioxidant, antispasmodic, cardiovascular, hypolipidemic, immunosuppressive, cytoprotective, antidiarrheal, antimicrobial, anthelmintic, insecticidal, adulticidal, diuretic, antioxidant, genotoxic, and mutagenic activities. This review is an effort to explore the different phytoconstituents and pharmacological activities of Acorus calamus.

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“…The potential of this plant as an antifungal (Lee et al, 2004;Lee, 2007), antibacterial (McGraw et al, 2002;Phongpaichit et al, 2005), allopathic (Nawamaki and Kuroyanagi, 1996), anticellular and immunosuppressive (Mehrotra and Mishra, 2003) agent has been known for quite some time. Conversely, the magnitude of work centered at uncovering its true potential as an antifungal, anti-yeast and anti-oxidant agent does not stand at par with the gigantic biological potency of its rhizomes.…”

Section: Introductionmentioning

confidence: 99%

Rhizome extracts of Acorus odoratus: Antifungal, anti-yeast, anti-oxidant and HPLC quantification

Khan

Bakht

Khan

2017

Bangladesh J Pharmacol

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Different extracts from the rhizomes of Acorus odoratus were assessed for the presence of antifungal, anti-yeast and anti-oxidant potential. Mild antifungal and robust anti-yeast potential were revealed by the tested samples. A gigantic anti-oxidant activity, however, was noted for all extracts at each concentration used. The data further revealed that n-butanol and ethyl acetate fractions measured fair antifungal (41.5% inhibition against Rhizopus oryzae and 36.2% inhibition against Acremonium alternatum at 2000 µg/well respectively), and highest anti-yeast (70.3 and 66.8% activities respectively at 2000 µg/disc) and anti-oxidant (95.8 and 97.9% activities respectively at 250 ppm) potential while samples extracted with water were least potent. Furthermore, both n-butanol and ethyl acetate were found as the best extraction solvents for antifungal, anti-yeast and anti-oxidant compounds (particularly gallic acid) from the rhizomes of A. odoratus in HPLC analysis.

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Anticellular and immunosuppressive properties of ethanolic extract of Acorus calamus rhizome

Cited by 101 publications

References 25 publications

Evaluation of the proliferative activity of methanol extracts from six medicinal plants in murine spleen cells

Evaluation of the proliferative activity of methanol extracts from six medicinal plants in murine spleen cells

Acorus calamus.: Scientific Validation of Ayurvedic Tradition from Natural Resources

Rhizome extracts of <i>Acorus odoratus</i>: Antifungal, anti-yeast, anti-oxidant and HPLC quantification

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