Anticancer and antimetastatic activity of Hypomyces chrysospermus, a cosmopolitan parasite in different human cancer cells

Dikmen, Miriş; Öztürk, Selin Engür; Cantürk, Zerrin; Ceylan, Göksu; Karaduman, Ayşe Betül; Yamaç, Mustafa

doi:10.1007/s11033-020-05468-6

Cited by 8 publications

(3 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The sample was positioned on an RTCA station within the incubator for an extended period to quantify cellular proliferation. Cell proliferation and IC 50 concentrations of bortezomib and ixazomib were monitored and evaluated for 24 h according to the cell index values by RTCA DP Software (Agilent, version numbers 1.2.1) [22].…”

Section: Methodsmentioning

confidence: 99%

Enhanced angiogenesis of human umbilical vein endothelial cells via THP‐1‐derived M2c‐like macrophages and treatment with proteasome inhibitors ‘bortezomib and ixazomib’

Engür‐Öztürk,

Kaya‐Tİlkİ,

Cantürk

et al. 2024

APMIS

Self Cite

View full text Add to dashboard Cite

The leading cause of cancer‐related death is lung cancer, with metastasis being the most common cause of death. To elucidate the role of macrophages in lung cancer and angiogenesis processes, we established an in vitro co‐culture model of A549 or HUVEC with THP‐1 cells that polarized to M2c macrophages with hydrocortisone. The proteasome inhibitors bortezomib and ixazomib were investigated for their effects on proliferation, invasion, migration, metastasis, and angiogenesis pathways. The effects of bortezomib and ixazomib on gene expression in gene panels, including crucial genes related to angiogenesis and proteasomes, were investigated after the co‐culture model to determine these effects at the molecular level. In conclusion, bortezomib and ixazomib showed antiproliferative effects in both cells, as well as in M2c macrophage co‐culture. M2c macrophages also increased invasion in A549 cells and both invasion and migration in HUVEC. mRNA expression upregulation, specifically in the NFKB and VEGF genes, supported the metastatic and angiogenic effects found in A549 and HUVEC with M2c macrophage co‐culture. Additionally, bortezomib inhibited the VEGFB pathway in HUVEC and NFKB1 in A549 cells. The significant findings obtained as a result of this study will provide information regarding angiogenesis induced by M2 macrophages.

show abstract

Section: Methodsmentioning

confidence: 99%

Enhanced angiogenesis of human umbilical vein endothelial cells via THP‐1‐derived M2c‐like macrophages and treatment with proteasome inhibitors ‘bortezomib and ixazomib’

Engür‐Öztürk,

Kaya‐Tİlkİ,

Cantürk

et al. 2024

APMIS

Self Cite

View full text Add to dashboard Cite

show abstract

“…RTCA DP detects the cell number indirectly by measuring electrical impedance and generates real-time data by collecting measurements at predetermined intervals over a period of days [14][15][16]. In our study, the RTCA DP system was used for the real-time and time-dependent analysis of IC 50 concentrations of A549 and CCD-19Lu cells.…”

Section: Antiproliferative Effects Of Bortezomib and Ixazomib Determined By Rtca Dpmentioning

confidence: 99%

“…(BioTek, Winooski, VT, USA). The number of living cells was directly proportional to the absorbance, and cell viability was expressed as a percentage of controls [13,14].…”

Section: Wst-1 Cytotoxicity Testmentioning

confidence: 99%

Proteasome Inhibitor Immunotherapy for the Epithelial to Mesenchymal Transition: Assessing the A549 Lung Cancer Cell Microenvironment and the Role of M1, M2a and M2c ‘Hydrocortisone-Polarised’ Macrophages

Öztürk

Dikmen

2022

Preprint

Self Cite

View full text Add to dashboard Cite

Lung cancer is a leading cause of cancer-related deaths, primarily as a result of metastases. In this metastasis, the epithelial-to-mesenchymal transition (EMT) is essential. Interaction with the cancer cell microenvironment is primarily dependent on M1- and M2-polarized macrophage. In this study, we revealed the EMT-associated activity of M1, M2a and M2c macrophages in A549 lung cancer cells. We established a co-culture model of A549 lung cancer cells utilizing THP-1-derived M1/M2 polarised macrophages to explore the involvement of macrophages in the immune response, apoptosis, and EMT in lung cancer. Although multiple polarising agents are routinely used for M1 and M2 conversion, we assessed a new possible polarising agent, hydrocortisone. M1 increased A549 cell sensitivity to proteasome inhibitors and decreased A549 cell viability by inducing apoptosis. EMT was induced in the presence of M2c macrophages in A549 cells by the levels of vimentin, fibronectin, E-cadherin, NF-kB, CCL-17. We also revealed the antiproliferative effects of bortezomib and ixazomib on A549 cells in both 2D and 3D cultures. Our findings could help develop an immunotherapeutic strategy by shedding light on a previously undiscovered part of the EMT pathway. Furthermore, additional investigation may reveal that polarising tumour-associated macrophages to M1 and eliminating the M2a or particularly the M2c subtype are effective anti-cancer strategies.

show abstract

Proteasome inhibitor immunotherapy for the epithelial to mesenchymal transition: assessing the A549 lung cancer cell microenvironment and the role of M1, M2a and M2c ‘hydrocortisone-polarised’ macrophages

Öztürk

Dikmen

2022

Mol Biol Rep

View full text Add to dashboard Cite

Anticancer and antimetastatic activity of Hypomyces chrysospermus, a cosmopolitan parasite in different human cancer cells

Cited by 8 publications

References 23 publications

Enhanced angiogenesis of human umbilical vein endothelial cells via THP‐1‐derived M2c‐like macrophages and treatment with proteasome inhibitors ‘bortezomib and ixazomib’

Enhanced angiogenesis of human umbilical vein endothelial cells via THP‐1‐derived M2c‐like macrophages and treatment with proteasome inhibitors ‘bortezomib and ixazomib’

Proteasome Inhibitor Immunotherapy for the Epithelial to Mesenchymal Transition: Assessing the A549 Lung Cancer Cell Microenvironment and the Role of M1, M2a and M2c ‘Hydrocortisone-Polarised’ Macrophages

Proteasome inhibitor immunotherapy for the epithelial to mesenchymal transition: assessing the A549 lung cancer cell microenvironment and the role of M1, M2a and M2c ‘hydrocortisone-polarised’ macrophages

Contact Info

Product

Resources

About