Antibody formation in the transplanted spleen: a simple method of splenic transplantation in the rat using the cuff technique

Abstract

To study whether transplanted spleens produce antibodies or not, a simplified model of spleen transplantation using the cuff technique was developed. The whole spleen with vascular pedicles was implanted in the syngeneic DA (RT1a) rat combination, using the cuff technique applied to the renal artery and vein of the recipient. The functions of the grafted spleen were tested by antibody formation-titers of antibody in the serum and plaque-forming cells in the graft spleen against xenogeneic [BALB/c (H-2d) mouse] antigen. The grafted spleens included a number of plaque-forming cells, as did the host spleen. This report shows direct evidence that vascularized splenic grafts produced antibodies. The technique is described in detail.

Laparoscopic hand-assisted spleen autotransplantation

“…The first open spleen transplantations also were performed in animal models [6,11]. The related studies showed the feasibility of the technique as well as the viability of the spleen transplant and its functional state [13].…”

Simplified method of heterotopic rat heart transplantation using the cuff technique: Application to sublethal dose protocol of methotrexate on allograft survival

“…Using the cuff method, this cervical auxiliary heart graft improved vascular anastomosis, with the advantage of no bleeding at the anastomosis site and easy examination of the graft beating. We have developed a cuff technique in rats [5][6][7][8][9] , with a considerable number of HHT performed at our center. [10][11][12][13][14] In these extensive series, we suggest that the initial experience of cuff method could be acquired through rat HHT procedures in the neck for a basic understanding of vessel anastomosis, using the cuff.…”

The Impact of Heterotopic Spleen Regeneration on Tumor Growth

“…So, SE, regardless of the reason for the operation, is a procedure that is characterized by serious consequences for the body as a whole. Consequently, a promising area of research is the study of potential spleen regeneration and its impact on the body as a whole [ 14 , 15 , 16 ]. The existence of such a condition as splenosis has long been known in the literature, and there is a solid basis for research into possible regeneration and its impact.…”