1990
DOI: 10.1002/jmr.300030304
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Abstract: The use of antibodies in immunoaffinity separations represents one of the most specific methods for purifying substances of biological interest. Since the binding affinity of antibody greatly influences its behavior in such separations, it is often important to know the value of the antibody affinity expressed as an equilibrium constant K. The present review discusses the equations used in the quantitative analysis of antigen/antibody interactions and describes currently used experimental methods for measuring… Show more

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Cited by 42 publications
(22 citation statements)
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References 58 publications
(51 reference statements)
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“…Various surface effects have been considered as possible explanations for differences in affinities in solid-phase assays [12][13][14]. Coating of protein on microtitre plate wells may change conformation or even lead to denaturation [15].…”
Section: Discussionmentioning
confidence: 99%
“…Various surface effects have been considered as possible explanations for differences in affinities in solid-phase assays [12][13][14]. Coating of protein on microtitre plate wells may change conformation or even lead to denaturation [15].…”
Section: Discussionmentioning
confidence: 99%
“…β 3 has been used as a measure of affinity (Azimzadeh and Rongenmortel, 1990) and reciprocal of β 3 was used as the apparent association constant ( K a ). The molecular weight of d‐dimer was assumed to be 228 kD in the calculations.…”
Section: Resultsmentioning
confidence: 99%
“…The strength of the affinity is represented by the affinity constant. Equilibrium dialysis is the classical method used to determine the affinity constant, but it is only suitable for small molecule antigen and antibody, and the operations involved are quite complicated [18]. The solid phase non-competitive ELISA method for the determination of affinity constants is not limited by the antigen size and it is easy to operate, but some scholars believed that antigen-antibody reaction kinetics would be changed and the application of the Law of Mass Action would be limited, because the antigen-antibody reaction is transferred from a liquid phase to the solid-liquid interface, caused by the antigen has been coated in microtitre plate [19].…”
Section: Discussionmentioning
confidence: 99%