Antibiotics and common antibacterial biocides stimulate horizontal transfer of resistance at low concentrations

Jutkina, Jekaterina; Marathe, Nachiket P.; Flach, Carl-Fredrik; Larsson, D. G. Joakim

doi:10.1016/j.scitotenv.2017.10.312

Cited by 259 publications

(141 citation statements)

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“…The HAR method was faster and easier to perform than conventional scrubbing. Emergence of resistance to CHX has been observed in clinical populations, and this could translate into cross resistance to antibiotics . Currently, there is no known resistance to HA solutions, and this method with HA gel might prevent the emergence of resistant bacteria.…”

Section: Discussionmentioning

confidence: 99%

“…Emergence of resistance to CHX has been observed in clinical populations, and this could translate into cross resistance to antibiotics. 15,16 Currently, there is no known resistance to HA solutions, and this method with HA gel might prevent the emergence of resistant bacteria. The efficiency of this asepsis method should now be investigated in clinical situations in which the transient skin flora is more abundant and more heterogeneous.…”

Section: Discussionmentioning

confidence: 99%

“…To preserve the efficacy of antiseptic agents in controlling infections, it is essential to prevent the emergence of bacteria resistant to these compounds as well as cross resistance by using these antimicrobial compounds in an appropriate manner . An emergence of resistance to CHX has been observed in clinical populations, which could translate into cross resistance to antibiotics . Currently, there is no known resistance to hydroalcoholic (HA) solutions.…”

Section: Introductionmentioning

confidence: 99%

“…13,14 An emergence of resistance to CHX has been observed in clinical populations, which could translate into cross resistance to antibiotics. 15,16 Currently, there is no known resistance to hydroalcoholic (HA) solutions.…”

Section: Introductionmentioning

confidence: 99%

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Comparison of hydroalcoholic rubbing and conventional chlorhexidine scrubbing for aseptic skin preparation in dogs

et al. 2019

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Objective To compare preparation time, ease of application, and elimination of skin contamination of 3 skin preparation methods for asepsis. Study design Experimental study. Animals Healthy dogs (n = 6) with no clinical signs of skin disease. Methods Three sites on each dog were randomly allocated to 1 of 3 preparation protocols for asepsis: (1) 5 scrubbings with chlorhexidine gluconate and rinsing (CHXG), (2) washing with mild soap prior to 3 rubbings with hydroalcoholic solution (soap‐HAR), or (3) 3 rubbings with hydroalcoholic solution (HAR). The duration of each method of skin preparation was recorded. A Count‐Tact agar plate was placed in the center of each site before, immediately after, 1 hour after, and 3 hours after antiseptic application. Plates were cultured, and colony forming units (CFU) were counted. Results Skin preparation lasted an average of 375 seconds for CHXG, 240 seconds for soap‐HAR, and 190 seconds for HAR (P = .00049). Nine CFU (median) were cultured from the skin prior to preparation, with no difference between sites on any animal or for any method. Colony forming units were not detected at any time on any site in any dog after antiseptic application. Conclusion Rubbing with hydroalcoholic (HA) solution was as effective as CHXG and prevented bacterial growth for at least 3 hours under these experimental conditions. Rubbing with hydroalcoholic solution was also faster and easier to perform. Clinical significance Because there is currently no known resistance to HA solution, preparation of the surgical site with HAR should be considered to prevent the emergence of bacterial resistance to chlorhexidine as well as potential cross‐resistances to antibiotics. Transfer to clinical animals requires additional investigation.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comparison of hydroalcoholic rubbing and conventional chlorhexidine scrubbing for aseptic skin preparation in dogs

et al. 2019

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“…At a continental scale, ARGs in sediments are strongly correlated with MGEs and antibiotic residues (6). Recently, it has been observed that microorganisms living in aquatic microbial communities that came from wastewater were able to transfer ARGs via horizontal gene transfer (HGT) after exposure to low levels of antibiotics and biocides (7). Many of the ARGs that can be found in clinical settings have also been found in the environment, suggesting the possibility of movement and dissemination between these two scenarios (8).…”

Section: Introductionmentioning

confidence: 99%

Antibiotic resistance genes and class 1 integron: Evidence of fecal pollution as a major driver for their abundance in water and sediments impacted by metal contamination and wastewater in the Andean region of Bolivia

Agramont

Gutiérrez-Cortez

Joffré

et al. 2020

Preprint

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26Water and sediment samples affected by mining activities were collected from 27 three lakes in Bolivia, the pristine Andean lake Pata Khota, the Milluni Chico lake 28 directly impacted by acid mine drainage, and the Uru-Uru lake located close to 29 Oruro city and highly polluted by mining activities and human wastewater 30 discharges. Physicochemical parameters, including metal compositions, were 31 analyzed in water and sediment samples. Antibiotic resistance genes (ARGs), 32 were screened for, and verified by quantitative PCR together with the mobile 33 element class 1 integron (intl1) as well as crAssphage, a marker of human fecal 34 pollution. The gene intl1 showed a positive correlation with sul1, sul2, tetA and 35 blaOXA-2. CrAssphage was only detected in Uru-Uru lake and its tributaries and 36 significantly higher abundance of ARGs were found in these sites. Multivariate 37 analysis showed that crAssphage abundance, electrical conductivity and pH were 38 positively correlated with higher levels of intl1 and ARGs. Taken together our 39 results suggest that fecal pollution is the major driver of higher ARGs and intl1 in 40 wastewater and mining contaminated environments. 41 3 48 can be incorporated and replicated in environmental microorganisms, thereby 49 increasing their concentration (3). 50 It has been reported that anthropogenic activities cause pollution of aquatic 51 environments with ARGs and MGEs (4). Wastewater discharges cause co-52 occurrence of MGEs and different ARGs in water and sediments (5). At a 53 continental scale, ARGs in sediments are strongly correlated with MGEs and 54antibiotic residues (6). Recently, it has been observed that microorganisms living in 55 aquatic microbial communities that came from wastewater were able to transfer 56 ARGs via horizontal gene transfer (HGT) after exposure to low levels of antibiotics 57 and biocides (7). Many of the ARGs that can be found in clinical settings have also 58 been found in the environment, suggesting the possibility of movement and 59 dissemination between these two scenarios (8). 60Mining activities cause contamination of downstream water with dissolved 61 metals (9) where heavy metals tend to accumulate in sediments (10). It has been 62 suggested that heavy metals can favor selection of ARGs via co-selection, i.e. the 63 simultaneous acquisition of both, ARG and metal resistance genes, where the 64 presence of metals constitutes the selective pressure (11). Several studies support 65 this relation. Urban soil samples of Belfast in Northern Ireland, exhibited a pattern 66 of co-occurrence between metals (Zn, Cu, Cd, Co, Ni, Hg, Cr and As) and many 67 ARGs. Moreover, the degree of metal toxicity was positively correlated with the 68 abundance of MGEs, and ARGs (12). Metals, as Cu and Zncan in some cases 69 exert stronger selection pressure over soil microbial communities for the selection 70 of resistant bacteria, even more than specific antibiotics (13). In the Dongying river 71 in China, the levels of Cu and Cr were positively c...

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Strategies toward development of antimicrobial biomaterials for dental healthcare applications

Parhi

Pal

Das

et al. 2021

Biotech & Bioengineering

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Several approaches for elimination of oral pathogens are being explored at the present time since oral diseases remain prevalent affecting approximately 3.5 billion people worldwide. Need for antimicrobial biomaterials in dental healthcare include but is not restricted to designing resin composites and adhesives for prevention of dental caries. Constant efforts are also being made to develop antimicrobial strategies for clearance of endodontic space prior root canal treatment and for treatment of periimplantitis and periodontitis. This article discusses various conventional | © 2021 Wiley Periodicals LLC Microorganisms Type of disease References Lactobacilli Dental caries Caufield et al. (2015) (Mutans streptococci) Streptococcus mutans, Streptococcus sorbinus Dianawati et al. (2020) Aggregatibacter actinomycetemcomitans Periodontitis Oscarsson et al. (2019) Porphyromonas gingivalis Z. He et al. (2020) Fusobacterium nucleatum Endodontic infection Martinho et al. (2016); Matsui et al. (2014) Enterococcus faecalis Alghamdi and Shakir (2020)

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Antibiotics and common antibacterial biocides stimulate horizontal transfer of resistance at low concentrations

Cited by 259 publications

References 59 publications

Comparison of hydroalcoholic rubbing and conventional chlorhexidine scrubbing for aseptic skin preparation in dogs

Comparison of hydroalcoholic rubbing and conventional chlorhexidine scrubbing for aseptic skin preparation in dogs

Antibiotic resistance genes and class 1 integron: Evidence of fecal pollution as a major driver for their abundance in water and sediments impacted by metal contamination and wastewater in the Andean region of Bolivia

Strategies toward development of antimicrobial biomaterials for dental healthcare applications

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