Antibiotic Susceptibility Profile of Lactic Acid Bacteria with Probiotic Potential Isolated from Humans

Narayanan, Rakhie; Raghavan, Keerthi Thalakattil; Ahenkan, Albert; Apedzi, Comfort Michelle

doi:10.26717/bjstr.2019.17.003033

Cited by 4 publications

(4 citation statements)

References 5 publications

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“…The results of the present study showed that the use of antibiotics reduces the beneficial bacteria in the gut, including lactobacillus and bifidobacteria. Similarly, other colleagues achieved these results [26,29]. Antibiotic treatment also increased the severity of seizures and reduced the time required for seizures to occur in the seizure model caused by pentylene tetrazole.…”

Section: Discussionmentioning

confidence: 74%

The Effect of Intestinal Natural Micro Flora Removal on Susceptibility to Have Seizure in Male Wistar Rats

et al. 2020

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Background and Aim: Epilepsy is a common brain disorder. Brain function can be affected by the intestinal microflora. The intestinal microflora have a major role in modulating immune responses, producing essential metabolites and mediating neurotransmitters. Antibiotics can affect and reduce these roles and functions by eliminating the intestinal microflora. The aim of this study was to investigate the effect of intestinal natural micro flora removal on seizure susceptibility and seizure behavior modification with the use of probiotics in male Wistar rats. Methods & Materials: This study was performed on 32 male Wistar rats with weight range 200-250 gr. The animals were randomly divided into four groups: 1. Control group; 2. Antibiotic group; 3. Probiotic group and 4. Antibiotic + probiotic group. To remove the microflora, antibiotics (neomycin, ampicillin, and metronidazole) for three weeks and for replacement of microflora, probiotics (Lactobacilli casei, Lactobacillus acidophilus and Bifidobacterium bifidum) for four weeks were administered. Seizures were performed by intraperitoneal injection of pentylentrazole. The microflora was examined by the MRS Agar medium and the Pure Plate method. The data were statistically analyzed in Graph Pad Prism V. 8. Ethical Considerations: This study was approved by the Research Ethics Committee of Arak University of Medical Sciences (Code: IR.ARAKMU.REC.1395.176). Results: The use of antibiotics lead to decrease the number of intestinal bacteria (P<0.0001), increased the severity and stability of seizure stages (P<0.05) and decreased the time delay of seizure onset (P<0.05) compared to the control group. Probiotic consumption by modifying the intestinal microflora (P<0.0001) reduced the severity of seizure and increased the time delay of seizure onset (P<0.05). Conclusion: Elimination of microflora has the potential to induce seizures, which can be compensated by administration of probiotics.

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Section: Discussionmentioning

confidence: 74%

The Effect of Intestinal Natural Micro Flora Removal on Susceptibility to Have Seizure in Male Wistar Rats

et al. 2020

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“…After the plates were incubated, the diameter of the zone of inhibition of microbial growth was observed and measured in millimeters. Interpretation of the antibiotic susceptibility profile of LAB was performed according to previous publications [ 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 ]. The susceptibility was expressed in terms of susceptible (S), moderately susceptible (MS), and resistant (R).…”

Section: Methodsmentioning

confidence: 99%

Characterization and Protective Properties of Lactic Acid Bacteria Intended to Be Used in Probiotic Preparation for Honeybees (Apis mellifera L.)—An In Vitro Study

Leska

Nowak

Rosicka‐Kaczmarek

et al. 2023

Animals

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Lactic acid bacteria (LAB) are widely used probiotics and offer promising prospects for increasing the viability of honeybees. Thus, the probiotic potential of 10 LAB strains was determined, which in our previous studies showed the most potent protective abilities. In the current study, we investigated various properties of probiotic candidates. The tested LAB strains varied in susceptibility to tested antibiotics. Isolates showed high viability in sugar syrups and gastrointestinal conditions. None of the LAB strains exhibited β-hemolytic activity, mutual antagonism, mucin degradation, hydrogen peroxide production capacity, or bile salt hydrolase (BSH) activity. Additionally, the cytotoxicity of LAB cell-free supernatants (CFS) was assessed, as well as the effect of CFS from P. pentosaceus 14/1 on the cytotoxicity of coumaphos and chlorpyrifos in the Caco-2 cell line. The viability of Caco-2 cells reached up to 89.81% in the presence of the highest concentration of CFS. Furthermore, LAB metabolites decreased the cytotoxicity of insecticides (up to 19.32%) thus demonstrating cytoprotective activity. All tested LAB strains produced lactic, acetic, and malonic acids. This research allowed the selection of the most effective LAB strains, in terms of probiosis, for future in vivo studies aimed at developing an ecologically protective biopreparation for honeybees.

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“…Previously, Yadav et al [55] reported that all probiotics in the LAB showed resistance to vancomycin, and some isolates showed resistance to kanamycin. Resistance to kanamycin is due to a lack of cytochrome-mediated electron transport in LAB, which mediates the antibiotics' absorption [65]. Duche et al [66] found that L. paracasei with ermB and/or ermC was sensitive to both macrolide antibiotics.…”

Section: Antibiotic-resistant Probioticsmentioning

confidence: 99%

“…They also described how this resistance is considered a natural or intrinsic property, which is due to the presence of D-alanine: D-alanine ligase-related enzymes are used to separate them from other Gram-positive bacteria. The best-characterized resistance in LAB is its resistance to vancomycin, due to the lack of binding sites in peptidoglycan [65].…”

Section: Antibiotic-resistant Probioticsmentioning

confidence: 99%

Effects of Synbiotic Lacticaseibacillus paracasei, Bifidobacterium breve, and Prebiotics on the Growth Stimulation of Beneficial Gut Microbiota

Kaewarsar,

Chaiyasut,

Lailerd

et al. 2023

Foods

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The gut microbiota is a complex community of microorganisms that plays a vital role in maintaining overall health, and is comprised of Lactobacillus and Bifidobacterium. The probiotic efficacy and safety of Lacticaseibacillus paracasei and Bifidobacterium breve for consumption were confirmed by in vitro experiments. The survival rate of the probiotics showed a significant decline in in vitro gut tract simulation; however, the survival rate was more than 50%. Also, the probiotics could adhere to Caco-2 cell lines by more than 90%, inhibit the pathogenic growths, deconjugate glycocholic acid and taurodeoxycholic acid through activity of bile salt hydrolase (BSH) proteins, and lower cholesterol levels by over 46%. Regarding safety assessment, L. paracasei and B. breve showed susceptibility to some antibiotics but resistance to vancomycin and were examined as γ-hemolytic strains. Anti-inflammatory properties of B. breve with Caco-2 epithelial cell lines showed the significantly highest value (p < 0.05) for interleukin-10. Furthermore, probiotics and prebiotics (inulin, fructooligosaccharides, and galactooligosaccharides) comprise synbiotics, which have potential effects on the increased abundance of beneficial microbiota, but do not affect the growth of harmful bacteria in feces samples. Moreover, the highest concentration of short chain fatty acid was of acetic acid, followed by propionic and butyric acid.

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Antibiotic Susceptibility Profile of Lactic Acid Bacteria with Probiotic Potential Isolated from Humans

Cited by 4 publications

References 5 publications

The Effect of Intestinal Natural Micro Flora Removal on Susceptibility to Have Seizure in Male Wistar Rats

The Effect of Intestinal Natural Micro Flora Removal on Susceptibility to Have Seizure in Male Wistar Rats

Characterization and Protective Properties of Lactic Acid Bacteria Intended to Be Used in Probiotic Preparation for Honeybees (Apis mellifera L.)—An In Vitro Study

Effects of Synbiotic Lacticaseibacillus paracasei, Bifidobacterium breve, and Prebiotics on the Growth Stimulation of Beneficial Gut Microbiota

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