Antibiotic susceptibilities and prevalence of Methicillin resistant Staphylococcus aureus (MRSA) isolated from bovine milk in Pakistan

Aqib, Amjad Islam; Ijaz, Muhammad; Anjum, Aftab Ahmad; Malik, Muhammad Abdul; Mehmood, Khalid; Farooqi, Shahid Hussain; Hussain, Kashif

doi:10.1016/j.actatropica.2017.08.008

Cited by 47 publications

(45 citation statements)

References 24 publications

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“…The MRSA isolates from the field were highly sensitive to Moxifloxacin (95%), Gentamycin (90%) and Trimethoprim (87.7%) in line with the reported findings in the previous studies (Aqib et al, 2017). Penicillin groups including; Cloxacillin (100%), Ticarcillin (100%), Amoxicillin (100%), Oxacillin (100%), Ampicillin(100%) and Cefixime (100%) in Cephalosporin group was resistant to these isolates (Kulshrestha et al 2017).…”

Section: Discussionsupporting

confidence: 87%

“…Similar highlevel MRSA prevalence of 60%, was reported by Locatelli et al (2017). In contrast to our study lower MRSA prevalence (34%) in Pakistan (2017) was reported: while from other regions of the world a prevalence of 16.7%, and 9.2%, is reported from India (2011), Saudi Arabia (2018), respectively (Kumar et al, 2011;Aqib et al, 2017;El-Deeb et al, 2018). This higher MRSA prevalence in our study area could be due to the difference in management practices and geographical variations.…”

Section: Discussionsupporting

confidence: 66%

“…Such higher resistance might have developed due to the prolonged and haphazard use of antimicrobials (Kistler et al, 2018). Which is a result of plasmid transfer and transposons between Staphylococcal species highly prevalent in our environment (Aqib et al, 2017) and excessive production of beta-lactamase enzyme disturbing the function of antibiotic by breaking the beta-lactam ring (Adediran et al, 2018). The non-professional veterinary practice in the field is a serious threat to antibiotic resistance dramatic increase currently and in future if not controlled.…”

Section: Discussionmentioning

confidence: 99%

“…Regular monitoring is required for such rising concerns on human as well as animal side. MRSA causing sub-clinical mastitis are originated into animal body exposed to bad management and contaminated environment (Aqib et al, 2017).…”

Section: Research Articlementioning

confidence: 99%

“…The positive sample was thenprocess for an in-vitro therapeutic sensitivity test. In-vitro antibiotic susceptibility test: PCR confirmed (n=40) MRSA samples were carried out for antibiotic sensitivity following Kirby-Baur disc diffusion method, aseptically applied on Muller Hinton Agar by following reported procedure (Aqib et al, 2017). The antibiotic sensitivity against MRSA was processed for different classes of drugs used in the field, included penicillin, Cephalosporin, Sulphonamide, Amino-glycoside, and Quinolones group.…”

Section: Detection Of Mec-a Gene By Pcr Techniquementioning

confidence: 99%

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Epidemiology and Antimicrobial Susceptibility of Methicillin-Resistant Staphylococcus aureus in Cattle of Pothohar Region, Pakistan

Khan¹

2019

PVJ

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Sub-clinical mastitis has remained the leading cause for decline in production of dairy animals as a silent epidemic all the times in Pakistan. A questionnaire-based cross-sectional study was conducted to assess the prevalence and potential risk factors for sub-clinical mastitis in the Pothohar region of Pakistan in 2018. Total of 104 cattle milk samples were collected from commercial and subsistent dairy farms. CMT positive samples were cultured and biochemical tests were conducted before confirmed on PCR for mecA gene Methicillin Resistant Staphylococcus aureus (MRSA) bacteria. In-vitro antibiotic susceptibility was also assessed. An overall prevalence of 71.1% was found; where cross-bred was found more susceptible (80.7%) as compared to other breeds. MecA gene-MRSA prevalence based on PCR was 54%. On regression analysis the potential risk factors identified here included; daily milk yield, parity, udder shape, teat morphology, shed type, quarantine of new animals and deworming of animals (OR>1; P-value<0.05). In MRSA confirmed isolates, Penicillin group was found highly resistant (92.5%) amongst all the groups. While amongst individual antibiotics Amoxicillin was found highly (100%) resistant followed by Cefixime and Spectinomycin. Whereas based on sensitivity Quinolone (Moxifloxacin 95%) group was the most sensitive (91.7%) followed by Sulphonamides (Sulphaphenazole 87.5%) and Amino-glycoside (Gentamycin 90%). An emerging pattern of mecA gene MRSA was recorded here with alarming subclinical mastitis prevalence in the study area. Immediate preventive measures need to be taken to address the problem. The findings of the current study will assess in control and prevention of subclinical mastitis in Pakistan. A, 2019. Epidemiology and antimicrobial susceptibility of methicillin-resistant staphylococcus aureus in cattle of Pothohar Region, Pakistan. Pak Vet J, 39(3): 438-442. http://dx.

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Section: Discussionsupporting

confidence: 87%