Antibiotic resistance, phylogenetic typing, and virulence genes profile analysis of uropathogenic Escherichia coli isolated from patients in southern Iraq

Allami, Mohammed; Bahreini, Masoumeh; Sharifmoghadam, Mirza Mohammad Reza

doi:10.1007/s13353-022-00683-2

Cited by 17 publications

(18 citation statements)

References 45 publications

(31 reference statements)

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“…In addition, a worrisome finding from this study is a relatively high resistance rate for nitrofurantoin (14.81%) in isolates of E. coli . This is similar to a previous study demonstrating that E. coli isolates were resistant to nitrofurantoin (21%), 45 but in contrary to a few previous studies with a low prevalence of nitrofurantoin resistance in E. coli isolates. 46 , 47 The potential explanation of these resistance to antibiotics could be attributed to the inappropriate and excessive use of these antibiotics in the empirical treatment for E. coli- related infections in different parts of the world.…”

Section: Resultssupporting

confidence: 90%

Relationship Between Antibiotic Resistance, Biofilm Formation, and Biofilm-Specific Resistance in Escherichia coli Isolates from Ningbo, China

Qian¹,

Li²,

Yang³

et al. 2022

IDR

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Purpose Several Escherichia coli pathotypes still constitute an important public health concern owing to its pathogenicity and antimicrobial resistance. Moreover, biofilm formation of E. coli can allow the strains to interfere with host and antimicrobial eradication, thus conferring additional resistance. The association between the formation of biofilm and antimicrobial resistance determinants has been extensively exploited; nevertheless, there is still no definite conclusion. The purpose of this study was to provide additional data to augment the present knowledge about the subject. Methods Antibiotic resistance/susceptibility profiles of 81 isolates from pediatric individuals in China between 2011 and 2014 against 20 antibiotics were assessed using the VITEK 2 system. Biofilm-forming capacities were evaluated using the crystal violet staining method, confocal laser scanning microscopy (CLSM), and field emission scanning electron microscopy. Biofilm compositions inside the biofilm formed by representative strains were assessed using CLSM. The effects of antibiotics on biofilms generated by E. coli strains of different biofilm-forming ability were examined using CLSM in combination with gatifloxacin. The relationships between antibiotic resistance, biofilm formation, and biofilm-specific resistance in E. coli isolates were investigated. Results The results showed that 23 isolates were classified as multidrug-resistant, and 57 isolates were classified as extensively drug-resistant (XDR). Among the 69 isolates with the ability to form biofilms, 46 isolates were stronger biofilm formers. Correlation analysis demonstrated that strain populations exhibiting more robust biofilm formation likely contained larger proportions of XDR isolates. Conclusion Together, our study implies that there was an association between biofilm-formation and resistance to several antibiotics for XDR- E. coli isolates, and would provide novel insights regarding the prevention and treatment against E. coli -related infections.

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Section: Resultssupporting

confidence: 90%

Relationship Between Antibiotic Resistance, Biofilm Formation, and Biofilm-Specific Resistance in Escherichia coli Isolates from Ningbo, China

Qian¹,

Li²,

Yang³

et al. 2022

IDR

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“…In our study, phylogenetic group B2 had the highest prevalence among the UPEC strains with strains belonging to group A being the second most prevalent among the strains. These results are consistent with other studies in Iran ( 32 ) and Iraq ( 15 ). Commensal strains may become virulent by random functional point mutations adaptive for pathogenic environments and a genomic deletion that enhances pathogenicity.…”

Section: Discussionsupporting

confidence: 94%

“…The highest sensitivity of the strains was found against amikacin, meropenem, and imipenem which is consistent with other findings in Ethiopia ( 14 ), Iraq ( 15 ), and Turkey ( 12 , 18 ). In contrast, high resistance was found against erythromycin, ticarcillin, ampicillin, and piperacillin, a finding which is commonly reported for UPEC strains in Iraq ( 15 , 19 ), Romania ( 4 ), Turkey ( 12 ), Thailand ( 20 ), and India ( 21 ) and it could be partly due to the common use of these antibiotics for treatment of UTI in these countries. The high level of ESBL-positive UPEC in our study is of great concern and we found similar results in less developed countries ( 22 – 24 ).…”

Section: Discussionsupporting

confidence: 91%

“…The results of multidrug resistance showed that 60% of the strains were MDR. The prevalence of MDR-UPEC strains in some countries such as Mexico 92.7% ( 25 ), Thailand 62% ( 23 ), China 77.2% ( 26 ) and southern Iraq 94% ( 15 ) was higher than our result, while Nepal 51% ( 2 ) and Turkey 34.6% ( 12 ) reported lower results than ours. In recent years, there has been an increase in drug resistance of E. coli strains around the world.…”

Section: Discussioncontrasting

confidence: 86%

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Phylogenetic groups, serogroups and virulence factors of uropathogenic Escherichia coli isolated from patients with urinary tract infection in Baghdad, Iraq

Mohammed¹,

Hasan²,

Allami³

2022

IJM

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Background and Objectives: Uropathogenic Escherichia coli (UPEC) is divided into different phylogenetic groups that differ in their antibiotic resistance patterns, serogroups and pathogenicity. This study aimed to investigate the prevalence of phylogenetic groups of UPEC isolates and their relationship with serogroups and virulence factors in patients with UTIs. Materials and Methods: Of the 412 urine samples tested a total of 150 UPEC were isolated and confirmed with PCR using 16S rRNA gene. Antibiotic resistance of the isolates was tested using disk diffusion method and the isolates were divided into phylogenetic groups by the quadruplex PCR method. The prevalence of serogroups and virulence genes were investigated using multiplex PCR. Results: 87 (58%) of the isolates belonged to phylogroup B2. Virulence genes fimH (95.3%), aer (49.3%) and serogroups O8 (22.3%), O25 (21.5%) showed the highest prevalence. The lowest drug resistance was observed against imipenem (4.6%) and meropenem (3.3%). The prevalence of multidrug-resistant and extended-spectrum beta-lactamases isolates were 60% and 61.3%, respectively. We also found a significant relationship between phylogenetic groups, serogroups and virulence factors among our isolates. Conclusion: The high abundance of phylogenetic group B2, serogroups O8 and O25, and virulence genes fimH and aer indicate their importance in the pathogenesis of UPEC in this country.

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“…по отношению к фосфомицину устойчивость для этих групп была высокой и низкой соответственно. рядом исследователей отмечены аналогичные результаты по превалирующей частоте встречаемости резистентности к абп у представителей упэк филогенетической группы B2 [5,18], а также подгруппы B2 3 [19].…”

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Antibiotic Resistance of Uropathogenic <i>Escherichia coli</i> Isolated from Patients with Urinary Tract Infections at the Urological Inpatient Facility of the Saratov Clinical Hospital

Казанцев

Proskuryakova

Kazakova

et al. 2022

Problemy Osobo Opasnykh Infektsii

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The aim of the work was to study the profile of antibiotic resistance of uropathogenic Escherichia coli strains isolated from patients with urinary tract infections in the urological inpatient facility of the clinical hospital in the Saratov city, depending on appurtenance to phylogenetic groups and subgroups, as well as O-serogroups.Materials and methods. We assessed sensitivity/resistance to 25 different antibacterial drugs in 102 strains of uropathogenic E. coli. The studies were carried out using the disk diffusion method. The production of extended spectrum beta-lactamases was evaluated by the double disk method. Carbapenemase output was determined using the CIM test. The PCR method was applied to determine appurtenance to phylogenetic groups and subgroups, O-serogroups, as well as the frequency of occurrence of the mcr‑1, mcr‑2, mcr‑3, mcr‑4, mcr‑5 genes encoding the proteins that mediate the development of resistance to colistin.Results and discussion. It has been established that all strains of uropathogenic E. coli are more or less resistant to antibacterial drugs. All studied 102 strains showed resistance to 23 antibacterial drugs from 8 functional groups. The resistance of uropathogenic E. coli had certain differences depending on belonging to phylogenetic groups and subgroups, O-serogroups. Strains of uropathogenic E. coli with high resistance (up to 100 %) belonged to the B23 phylogenetic group, the main representatives of which are cultures of the most common O-25 serogroup. The production of extended-spectrum beta-lactamases has been phenotypically confirmed for 69 (67.6 %) strains. No carbapenemaseproducing cultures were found in the study. The mcr‑1 and mcr‑2 genes encoding resistance to colistin have been identified in 3 uropathogenic E. coli strains (2.9 %).

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Antibiotic resistance, phylogenetic typing, and virulence genes profile analysis of uropathogenic Escherichia coli isolated from patients in southern Iraq

Cited by 17 publications

References 45 publications

Relationship Between Antibiotic Resistance, Biofilm Formation, and Biofilm-Specific Resistance in Escherichia coli Isolates from Ningbo, China

Relationship Between Antibiotic Resistance, Biofilm Formation, and Biofilm-Specific Resistance in Escherichia coli Isolates from Ningbo, China

Phylogenetic groups, serogroups and virulence factors of uropathogenic Escherichia coli isolated from patients with urinary tract infection in Baghdad, Iraq

Antibiotic Resistance of Uropathogenic <i>Escherichia coli</i> Isolated from Patients with Urinary Tract Infections at the Urological Inpatient Facility of the Saratov Clinical Hospital

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