Antibiotic resistance pattern of Acinetobacter baumannii from burns patients: increase in prevalence of blaOXA-24-like and blaOXA-58-like genes

Tafreshi, Niloofar; Babaeekhou, Laleh; Ghane, Maryam

doi:10.18502/ijm.v11i6.2222

Cited by 17 publications

(15 citation statements)

References 25 publications

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“…In contrast, OXA-24 had a high expression rate in Ab strains from both groups, but its expression level in the CRAB group was lower than that in the CSAB group. This differs from the results of most studies showing that OXA-24 was highly expressed in MDR-Ab [14,17,18,32]. The thought was that this might be associated with a small sample size, but it might be that the expression trend of OXA-24 in Ab strains from the PICU of our hospital was indeed different from that of other regions; this needs to be validated in a subsequent larger-sized study.…”

Section: Table 2 (Continued)contrasting

confidence: 96%

Mechanical ventilation-associated pneumonia caused by Acinetobacter baumannii in Northeast China region: analysis of genotype and drug resistance of bacteria and patients’ clinical features over 7 years

Zhang

et al. 2021

Antimicrob Resist Infect Control

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Objective To investigate the clinical features and outcomes of patients with mechanical ventilation-associated pneumonia (VAP) caused by Acinetobacter baumannii (Ab), and to characterize the drug resistance of pathogenic strains and carbapenem resistance-associated genes. Methods Clinical data were collected from the PICU of Shengjing Hospital. Patients who met the diagnostic criteria of VAP and for whom Ab was a pathogen were selected as study participants. The patients were divided into carbapenem-resistant A. baumannii (CRAB) and carbapenem-sensitive A. baumannii (CSAB) groups. The genes closely associated with Ab resistance to carbapenems and the efflux pump-related genes were detected by real-time polymerase chain reaction, and results compared between the two groups. Results The total mechanical ventilation time and the administration time of antibiotics after a diagnosis of Ab infection were significantly higher in the CRAB group. And the CRAB group strains were only sensitive to amikacin, cephazolin, compound sulfamethoxazole, and tigecycline. Genetic test results indicated that IPM expression was not significantly different between two groups. The OXA-51 and OXA-23 in the CRAB group was markedly higher than that in the CSAB group, while OXA-24 expression was markedly lower. The expression of AdeABC and AdeFGH was significantly greater in the CRAB compared to CSAB group. Conclusion In pediatric patients with VAP caused by Ab infection, the detection rate of CRAB strains is far higher than that of CSAB strains; The abnormal expression of β-lactamase-producing genes (OXA-23, OXA-24, and OXA-51) and efflux pump-related genes (AdeABC and AdeFGH) is closely related to the production of CRAB.

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Section: Table 2 (Continued)contrasting

confidence: 96%

Mechanical ventilation-associated pneumonia caused by Acinetobacter baumannii in Northeast China region: analysis of genotype and drug resistance of bacteria and patients’ clinical features over 7 years

Zhang

et al. 2021

Antimicrob Resist Infect Control

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“…Our findings are in agreement with the results reported by other studies. [27][28][29][30] The bla OXA-51-like is an intrinsic carbapenemase produced by A. baumannii, while the high prevalence of bla OXA-23-like could be explained by its possible dissemination via horizontal gene transfer, 31 as several studies have shown that this gene is carried by transposons and conjugative plasmids. [32][33][34][35] Overexpression of OXA-51 and OXA-23 has been associated with insertion (IS) elements located upstream of their gene.…”

Section: Discussionmentioning

confidence: 99%

Molecular epidemiology of carbapenem‐resistant Acinetobacter baumannii isolated from three major hospitals in Jordan

Ababneh

Aldaken

Jaradat

et al. 2021

Int J Clinical Practice

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Background: In the last decade, incidences of carbapenem-resistant Acinetobacter baumannii have been increasingly reported worldwide. Consequently, A. baumannii was included in the World Health Organization's new list of critical pathogens, for which new drugs are desperately needed. The objective of this research was to study the molecular epidemiology and antimicrobial susceptibility of clinical carbapenemresistant A. baumannii isolated from Jordanian hospitals. Methods: A total of 78 A. baumannii and 8 Acinetobacter spp. isolates were collected from three major hospitals in Jordan during 2018. Disc diffusion and microdilution methods were used to test their susceptibility against 19 antimicrobial agents.Multilocus sequence typing (MLST) was performed using the Pasteur scheme, followed by eBURST analysis for all isolates. PCR was used to detect β-lactam resistance genes, bla OX A-23-like , bla OX A-51-like , and bla NDM-1 .Results: Of the 86 tested isolates, 78 (90.6%) exhibited resistance to carbapenems, whereas no resistance was recorded to tigecycline or polymyxins. Based on the resistance profiles, 10.4% and 84.8% of isolates were classified into multidrug resistant (MDR) or extensively drug resistant (XDR), respectively. The most prevalent carbapenems resistance genes amongst isolates were bla OX A-51-Like (89.5%), followed by bla OXA-23-Like (88.3%) and bla NDM-1 (10.4%). MLST revealed the presence of 19 sequence types (STs), belonging to eight different international complexes. The most commonly detected clonal complex (CC) was CC2, representing 64% of all typed isolates.Conclusions: This is the first study to report the clonal diversity of A. baumannii isolates in Jordan. A high incidence of carbapenem resistance was detected in the isolates investigated. In addition, our findings provided evidence for the widespread of bla OXA-23-like harbouring carbapenem-resistant A. baumannii and belonging to CC2.The number of XDR isolates identified in this study is alarming. Thus, periodic surveillance and molecular epidemiological studies of resistance factors are important to improve treatment outcomes and prevent the spread of A. baumannii infections.

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“…Analysis of AR of several A. baumannii isolates collected from different intensive care units revealed high resistance to the most commonly used antibiotics [ 16 , 17 ]. Several studies reported high carbapenem, aminoglycoside and colistin resistance of A. baumannii strains [ 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 ] ( Table 1 ). The carbapenem resistance was associated with the overexpression of OXA-51 [ 32 ] and OXA-23 [ 33 , 34 ] class D carbapenemases as well as of metallo-β-lactamases (MBLs) [ 35 ].…”

Section: Ar Profiles Of a Baumannii Strainsmentioning

confidence: 99%

Antibiotic Resistance Profiles, Molecular Mechanisms and Innovative Treatment Strategies of Acinetobacter baumannii

et al. 2020

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Antibiotic resistance is one of the biggest challenges for the clinical sector and industry, environment and societal development. One of the most important pathogens responsible for severe nosocomial infections is Acinetobacter baumannii, a Gram-negative bacterium from the Moraxellaceae family, due to its various resistance mechanisms, such as the β-lactamases production, efflux pumps, decreased membrane permeability and altered target site of the antibiotic. The enormous adaptive capacity of A. baumannii and the acquisition and transfer of antibiotic resistance determinants contribute to the ineffectiveness of most current therapeutic strategies, including last-line or combined antibiotic therapy. In this review, we will present an update of the antibiotic resistance profiles and underlying mechanisms in A. baumannii and the current progress in developing innovative strategies for combating multidrug-resistant A. baumannii (MDRAB) infections.

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Antibiotic resistance pattern of Acinetobacter baumannii from burns patients: increase in prevalence of blaOXA-24-like and blaOXA-58-like genes

Cited by 17 publications

References 25 publications

Mechanical ventilation-associated pneumonia caused by Acinetobacter baumannii in Northeast China region: analysis of genotype and drug resistance of bacteria and patients’ clinical features over 7 years

Mechanical ventilation-associated pneumonia caused by Acinetobacter baumannii in Northeast China region: analysis of genotype and drug resistance of bacteria and patients’ clinical features over 7 years

Molecular epidemiology of carbapenem‐resistant Acinetobacter baumannii isolated from three major hospitals in Jordan

Antibiotic Resistance Profiles, Molecular Mechanisms and Innovative Treatment Strategies of Acinetobacter baumannii

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