Antibiotic Resistance of Human Periodontal Pathogen Parvimonas micra Over 10 Years

Abstract

Changes were evaluated over 10 years in the in vitro resistance of human periodontopathic strains of Parvimonas micra to four antibiotics. Subgingival biofilms culture positive for P. micra from 300 United States adults with severe periodontitis in 2006, and from a similar group of 300 patients in 2016, were plated onto anaerobically incubated enriched Brucella blood agar alone, or supplemented with either doxycycline (4 mg/L), clindamycin (4 mg/L), amoxicillin (8 mg/L), or metronidazole (16 mg/L). P. micra growth on antibiotic-supplemented media indicated in vitro resistance to the evaluated antibiotic concentration. P. micra resistance was significantly more frequent among patients in 2016, as compared to 2006, for doxycycline (11.3% vs. 0.3% patients; 37.7-fold increase), and clindamycin (47.3% vs. 2.0% patients; 23.7-fold increase) (both p < 0.001), whereas resistance to amoxicillin (2.3% vs. 1.0% patients) and metronidazole (0% vs. 0.3% patients) remained low and statistically unchanged between the two patient groups (p-values > 0.05). No P. micra isolates in 2006 or 2016 were jointly resistant in vitro to both amoxicillin and metronidazole. The alarming increases in subgingival P. micra resistance to doxycycline and clindamycin raise serious questions about the empiric use of these antibiotics, either locally or systemically, in the treatment of United States periodontitis patients harboring subgingival P. micra.

Evaluation of the strategies to reduce third‐generation oral cephalosporins in dentistry at a Japanese academic hospital: An interrupted time series analysis

“…To our knowledge, there is one report that evaluated the antibiotic resistance of periodontal pathogens over time. This report showed that Parvimonas micra resistance to AMPC remained low in 2016 compared with 2006 (2.3% vs. 1.0% of patients) 15 …”

“…This report showed that Parvimonas micra resistance to AMPC remained low in 2016 compared with 2006 (2.3% vs. 1.0% of patients). 15 Thus, it is difficult to posit that antimicrobial resistance was the cause. Second, the difference in prophylactic antibiotics alone may not be sufficient to control clinical outcomes in the real world.…”

A case report of sepsis and death caused by Parvimonas micra, a rare anaerobe

“…Both of these underlying conditions can cause severe disruption of and a reduction in the strength of a patient's autoimmune system, which likely led to infection of this patient's lower back with P. micra at first. For the treatment of P. micra infection, penicillin, metronidazole, and clindamycin are usually preferred ( 8 ). Our patient was initially treated with a 3-day infusion of piperacillin–tazobactam, but her infection was not controlled.…”

A Review of Antimicrobial Activity of Dental Mesenchymal Stromal Cells: Is There Any Potential?

“…The occurrence of antibiotic resistance increased within the last decades and is one of the biggest challenges in medicine, particularly in dentistry [81][82][83]. The development of alternative strategies for antimicrobial defense is one of the challenges of contemporary dental research [84].…”