Antibiotic resistance and detection of the sul2 gene in urinary isolates of Escherichia coli in patients from Brazil

Teichmann, Aline; Agra, Homero; Nunes, Luciana Neves; Rocha, Marion Pereira da; Renner, Jane Dagmar Pollo; Possuelo, Lia Gonçalves; Carneiro, Marcelo; Rieger, Alexandre; Benitez, Lisianne Brittes; Valim, Andréia Rosane de Moura

doi:10.3855/jidc.3380

Cited by 21 publications

(11 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In Southern Brazil we have little recent data concerning the antimicrobial resistance of ExPEC. We had higher resistance profiles in ExPEC compared to commensal isolates, particularly with respect to resistance to fluoroquinolones (ciprofloxacin and levofloxacin) and trimethoprim-sulfamethoxazole (both at approximately 1/3 of strains being resistant) compared to other studies [24] (Figure 1). Among the ExPEC strains we tested, 28% were resistant to three or more antimicrobials.…”

Section: Discussionmentioning

confidence: 62%

See 1 more Smart Citation

Presence of virulence genes and pathogenicity islands in extraintestinal pathogenic Escherichia coli isolates from Brazil

Cyoia

Rodrigues

Nishio

et al. 2015

J Infect Dev Ctries

View full text Add to dashboard Cite

Introduction: Extraintestinal pathogenic Escherichia coli (ExPEC) is associated with various diseases such as urinary tract infections, neonatal meningitis and septicemia. There are many virulence factors (VF) encoded by genes in ExPEC, including papC, papG, ecpA, iroN, fyuA, iutA, ompTp, tsh, hlyF, hlyA and iss. These virulence genes may be present in pathogenicity islands (PAI) or plasmids. Methodology: In this study, we analyzed the presence of VF encoding genes, PAI sequences and phylogenetic groups of 96 ExPEC strains isolated from the urine and blood of patients at the University Hospital of Londrina, and we compared them with 50 faecal commensal strains from healthy individuals. Results: The VF fyuA (65.60%) was detected in pathogenic strains and commensal strains (46%). A comparison of the distribution of ExPEC and commensal strains in the phylogenetic groups showed that more ExPEC strains belonged to group B2 whereas more of the commensal isolates belonged to group A. The distribution of the seven PAI sequences between commensal strains and ExPEC strains showed that PAI IV 536 was common in both ExPEC and commensal isolates. Conclusions: These results showed that the ExPEC strains that belonged to group B2 had more PAI sequences compared to those of the other groups, especially group B1, which had virulence genes but the lowest percentage of PAI sequences, which leads us to conclude that the virulence of ExPEC strains characterized as B2 is likely attributed to PAI encoded genes, whereas the virulence of ExPEC strains belonging to phylogenetic group B1 is likely due to plasmid encoded virulence genes.

show abstract

Section: Discussionmentioning

confidence: 62%

“…Antimicrobials that are widely prescribed for treatment of UTI episodes include beta-lactams, trimethoprimsulfamethoxazole, quinolones, fluoroquinolones and aminoglycosides [24]. In Southern Brazil we have little recent data concerning the antimicrobial resistance of ExPEC.…”

Section: Discussionmentioning

confidence: 99%

Presence of virulence genes and pathogenicity islands in extraintestinal pathogenic Escherichia coli isolates from Brazil

Cyoia

Rodrigues

Nishio

et al. 2015

J Infect Dev Ctries

View full text Add to dashboard Cite

show abstract

“…These above mentioned findings were consistent with the findings in our study. Teichmann et al (2014) indicated that sul2 gene was highly prevalent in TMP-STX resistant UPEC isolates up to 67% in their study. In this study, sul2 genes were present in 68% of TMP-STX resistant isolates showing that sul2 genes was more prevalent than two other sul genes.…”

Section: Discussionmentioning

confidence: 80%

Distribution of sul genes and their variants in uropathogenic Escherichia coli isolated from two hospitals of Sabah

Wang

Lai

Zaw

et al. 2016

Asian J. Med. Biol. Res

View full text Add to dashboard Cite

Sulphonamides resistant strains are highly prevalent in uropathogenic Escherichia coli (UPEC) isolates. Sul genes encode sulphonamide resistance and are present on transferrable plasmids. Integrons (IGNs) are genetic elements containing integrase gene, attl site and gene cassettes which carry multiple antibiotic resistant genes. Class 1 integrons have been extensively studied because these were most prevalent among clinical isolates. In this study, UPEC isolates were determined for the antibiotic susceptibility patterns to four antibiotics commonly used for urinary tract infections, which include co-trimethoxazole (TMP-STX). Distribution of sul genes and integrase1 gene (intI1) was studied in TMP-STX resistant UPEC isolates by using multiplex polymerase chain reaction (mPCR). Sul genes variants were investigated by DNA sequencing of the whole open reading frame of sul1 and sul2 genes and PCR product of sul3 gene. Sul1, sul2 and sul3 genes were prevalent in 37 (24.7%) of 150 UPEC isolates. IntI1 is positive in 22 sul genes positive isolates. Of six isolates positive with sul2 genes, sul2(a) and sul2(b) variants, which were described in the previous study, in the four isolates and the two isolates respectively were observed. This is the first mPCR which investigates the prevalence of three sul genes and intI1 in the UPEC clinical isolates from two hospitals of Sabah.Asian J. Med. Biol. Res. June 2016, 2(2): 213-220

show abstract

“…Adicionalmente, houve resistência frequente em gram-negativos para cefalosporinas de primeira geração. Esta situação está de acordo com a utilização ampla dessas medicações nos últimos anos para tratamento de ITU em gestantes e não-gestantes, acarretando aumento na resistência bacteriana [21][22][23][24][25] . Todos outros antibióticos mostraram ótimo padrão de suscetibilidade.…”

Section: Discussionunclassified

Prevalência de uropatógenos e sensibilidade antimicrobiana em uroculturas de gestantes do Sul do Brasil

Schenkel¹,

Dallé²,

Antonello³

2014

Rev. Bras. Ginecol. Obstet.

View full text Add to dashboard Cite

show abstract

Antibiotic resistance and detection of the sul2 gene in urinary isolates of Escherichia coli in patients from Brazil

Cited by 21 publications

References 17 publications

Presence of virulence genes and pathogenicity islands in extraintestinal pathogenic Escherichia coli isolates from Brazil

Presence of virulence genes and pathogenicity islands in extraintestinal pathogenic Escherichia coli isolates from Brazil

Distribution of sul genes and their variants in uropathogenic Escherichia coli isolated from two hospitals of Sabah

Prevalência de uropatógenos e sensibilidade antimicrobiana em uroculturas de gestantes do Sul do Brasil

Contact Info

Product

Resources

About