Antibacterial efficiency of the Sudanese Roselle (Hibiscus sabdariffa L.), a famous beverage from Sudanese folk medicine

Abstract

Background:Hibiscus sabdariffa L. is a plant native to tropical Africa and intensively cultivated in Sudan. Its calyces are widely consumed with many uses in Sudanese folk medicine.Materials and Methods:The dried calyces of H. sabdariffa were subjected to soak in 80% v/v methanol to get the methanolic extract, which was tested against five Gram-negative and three Gram-positive referenced bacterial strains using disc diffusion method. Selected bioactive phytochemical compounds were also investigated using qualitative methods.Results:The results of the antibacterial test indicate that the methanol extract of H. sabdariffa calyces contained effective antibacterial agent(s), revealed a considerable zone of inhibition against all tested Gram-negative and Gram-positive bacteria, and it was a competitor to gentamicin and greatly higher than penicillin which showed weak or no effect.Conclusion:The results of current investigation support the folk medicine application of this plant against different microbial ailments and suggest it as a promising source for new antibacterial agents.

A Rare Triterpenoidal Saponin Isolated and Identified fromTetraena simplex(L.) Beier &Thulin (Syn.Zygophyllum simplexL.)

“…[10,12] A plethora of studies revealed the presence of flavonoids, phenolic acids, lignans, triterpenoids and sterols in such family. [10,[17][18][19][20][21][22][23] For instance, Zaki and co-workers [23] applied multiplesolvents extraction to the aerial parts of Z. aegyptium A. Hosny sp. nov.…”

“…[25] It is distributed in North Africa and known in the Arab region as (Garmal and Hureim). [17] It was found that it belongs to genus Tetraena, a sister to genus Zygophyllum and recently reassigned to such genus and could be referred as Tetraena simplex (L.) Beier & Thulin (syn. Z. simplex L.) (Zygophyllaceae).…”

Detection of potential AcrAB-TolC multidrug efflux pump inhibitor in calyces extract of Hibiscus sabdariffa

“…The antimicrobial activity of H. sabdariffa has been reported against foodborne and food spoilage microorganisms, like E. coli O157:H7, is a major foodborne pathogen [23,37], also against Gram-negative bacteria such as Pseudomonas aeruginosa, Klebsiella pneumonia, Proteus vulgaris, Salmonella enterica and some multidrug resistant Salmonella strains, in addition to some Gram-positive bacteria such as Bacillus subtilis, S. aureus, Staphylococcus epidermis, and Staphylococcus cereus [24,25,37,38].…”

“…Finely powdered and extracted in a Soxhlet with 2 L of methanol for 48 h. The methanol was evaporated by vacuum pressure, the ×10 concentrated extract 10 mg/ml was used in further experiments [23,25,31].…”

“…The antimicrobial efficiency of H. sabdariffa L. against E. coli and some other Gram-negative bacteria has been demonstrated [23][24][25]. Combination of methanol extracts of H. sabdariffa L. with antibiotics enhances the activity of some antibiotics against resistant strain and standard strain of E. coli [26].…”