Antibacterial Drug Release Electrochemically Stimulated by the Presence of Bacterial Cells – Theranostic Approach

Gamella, María; Guz, Nataliia; Mailloux, Shay; Pingarrón, José M.; Katz, Evgeny

doi:10.1002/elan.201400473

Cited by 29 publications

(39 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…−60 mV on the modified electrode, Figure D. Overall, the stepwise electrode modification finally resulting in the inhibition of the bioelectrocatalytic process and its re‐activation upon removal of the GOx‐NPs species are similar to the systems recently reported by us . In the previously published systems, the linkers keeping GOx‐NPs were split and GOx‐NPs were removed by different biomolecular/biological signals, but not with the DNA‐signal.…”

Section: Figuresupporting

confidence: 83%

“…This approach has been already applied for activation of DNA computing systems by enzyme‐biocatalyzed reactions . The substance‐releasing systems, composed of the sensing and releasing electrodes connected to each other, were activated by enzyme‐substrates (e.g., glucose) , pH changes , immune‐complex formation and presence of bacterial cells . However, DNA signals have never been used as signals activating the release processes from bioelectrochemical systems.…”

Section: Figurementioning

confidence: 99%

“…This result can be explained by interception of glucose through the biocatalytic reaction of GOx localized in the external layer of the biomolecular assembly on the electrode surface, thus restricting glucose penetration to the internal layer of PQQ‐GDH. The GOx‐biocatalyzed reaction resulted in oxidation of glucose by O 2 , thus resulting in the formation of H 2 O 2 , but without production of current on the modified electrode . It should be noted that the amount of GOx on the electrode surface should be large enough to fully consume glucose not allowing its diffusion to PQQ‐GDH localized in the first layer on the electrode surface.…”

Section: Figurementioning

confidence: 99%

See 2 more Smart Citations

DNA Release from a Bioelectronic Interface Stimulated by a DNA Signal – Amplification of DNA Signals

2016

Self Cite

View full text Add to dashboard Cite

A new bioelectronic system activated by a DNA‐signal and releasing another DNA has been designed. The system was composed of two modified electrodes: one electrode functionalized with enzymes was activated with a DNA‐signal, while another electrode coated with an alginate film released pre‐loaded DNA species, when the first electrode was activated. The sensing electrode was functionalized with pyrroloquinoline quinone‐dependent glucose dehydrogenase (PQQ‐GDH) producing negative potential in the presence of glucose. This bioelectrocatalytic process was inhibited by the presence of glucose oxidase (GOx) at the external surface of the biomolecular system organized on the electrode surface through a DNA‐linker. GOx consumed incoming glucose and did not allow its penetration to the internal layer functionalized with PQQ‐GDH, thus leaving the electrode mute. The DNA‐signal resulted in the detachment of GOx and activated the process biocatalyzed by PQQ‐GDH due to penetration of glucose to the internal layer at the electrode surface. Finally, formation of a negative potential on the sensing electrode resulted in the activation of the second electrode and DNA release from the alginate matrix. The Sense‐and‐Act system allowed amplification of the DNA‐signal by releasing much higher amount of DNA comparing with the applied DNA‐signal. The DNA‐signal amplification will find applications in various biosensor and biomolecular computing systems.

show abstract

Section: Figuresupporting

confidence: 83%

Section: Figurementioning

confidence: 99%

Section: Figurementioning

confidence: 99%

See 1 more Smart Citation

DNA Release from a Bioelectronic Interface Stimulated by a DNA Signal – Amplification of DNA Signals

2016

Self Cite

View full text Add to dashboard Cite

show abstract

“…On the other hand, the PQQ electrodes will produce ap otential of approximately 0mVi ft he solution pumped to the electrochemical cells from the Fredkin gate does not contain NADH (logic value 0). Again, this process repeats the pattern of outputs Q and R generated by the Fredkin gate for different combinations of primary inputs A, B,a nd C.T he electrochemically stimulated alginated issolution, resultingf rom the conversion of Fe 3 + to Fe 2 + has been already applied forv arious signal-stimulated releasep rocesses, [59][60][61][62][63] including DNA release. In simpler terms, the potentials measured on the PQQ electrodes repeat the same logic as outputs Q and R after the Fredkin gate, thus conserving the logic of the outputs, but being measured differently.T he PQQ electrodes in both electrochemical cells were connected electrically to the alginate-modified electrodes loaded with DNA.…”

Section: Enzyme-based Fredkin Gate:electrochemical Outputsmentioning

confidence: 99%

Bioelectronic Interface Connecting Reversible Logic Gates Based on Enzyme and DNA Reactions

et al. 2016

Self Cite

View full text Add to dashboard Cite

It is believed that connecting biomolecular computation elements in complex networks of communicating molecules may eventually lead to a biocomputer that can be used for diagnostics and/or the cure of physiological and genetic disorders. Here, a bioelectronic interface based on biomolecule-modified electrodes has been designed to bridge reversible enzymatic logic gates with reversible DNA-based logic gates. The enzyme-based Fredkin gate with three input and three output signals was connected to the DNA-based Feynman gate with two input and two output signals-both representing logically reversible computing elements. In the reversible Fredkin gate, the routing of two data signals between two output channels was controlled by the control signal (third channel). The two data output signals generated by the Fredkin gate were directed toward two electrochemical flow cells, responding to the output signals by releasing DNA molecules that serve as the input signals for the next Feynman logic gate based on the DNA reacting cascade, producing, in turn, two final output signals. The Feynman gate operated as the controlled NOT gate (CNOT), where one of the input channels controlled a NOT operation on another channel. Both logic gates represented a highly sophisticated combination of input-controlled signal-routing logic operations, resulting in redirecting chemical signals in different channels and performing orchestrated computing processes. The biomolecular reaction cascade responsible for the signal processing was realized by moving the solution from one reacting cell to another, including the reacting flow cells and electrochemical flow cells, which were organized in a specific network mimicking electronic computing circuitries. The designed system represents the first example of high complexity biocomputing processes integrating enzyme and DNA reactions and performing logically reversible signal processing.

show abstract

“…PQQ is a well‐known electrocatalyst for the NADH oxidation, particularly when it is used in biofuel cells . It should be noted that the NADH oxidation electrocatalyzed by PQQ proceeds without the application of an external potential and the process proceeds spontaneously resulting in the formation of a negative potential of ca.‐60 mV (vs. Ag/AgCl reference; the same reference was used in all other electrochemical experiments) on the electrode . This negative potential was used in our previous studies to dissolve electrochemically an Fe 3+ ‐crosslinked alginate gel thin film on a connected electrode, resulting in the release of biomolecules entrapped in the alginate gel .…”

Section: Figurementioning

confidence: 99%

An Enzyme‐based 1:2 Demultiplexer Interfaced with an Electrochemical Actuator

et al. 2016

Self Cite

View full text Add to dashboard Cite

An enzyme-based 1:2 demultiplexer is designed in a flow system composed of three cells where each one is modified with a different enzyme: hexokinase, glucose dehydrogenase and glucose-6-phosphate dehydrogenase. The Input signal activating the biocatalytic cascade is represented by glucose, while the Address signal represented by ATP is responsible for directing the Input signal to one of the output channels, depending on the logic value of the Address. The biomolecular 1:2 demultiplexer is extended to include two electrochemical actuators releasing entrapped DNA molecules in the active output channel. The modular design of the system allows for easy exchange and extension of the functional elements. The present demultiplexer can be easily integrated in various biomolecular logic systems, including different logic gates based on the enzyme- or DNA-based reactions, as well as containing different chemical actuators, for example, with a biomolecular release function.

show abstract

Antibacterial Drug Release Electrochemically Stimulated by the Presence of Bacterial Cells – Theranostic Approach

Cited by 29 publications

References 41 publications

DNA Release from a Bioelectronic Interface Stimulated by a DNA Signal – Amplification of DNA Signals

DNA Release from a Bioelectronic Interface Stimulated by a DNA Signal – Amplification of DNA Signals

Bioelectronic Interface Connecting Reversible Logic Gates Based on Enzyme and DNA Reactions

An Enzyme‐based 1:2 Demultiplexer Interfaced with an Electrochemical Actuator

Contact Info

Product

Resources

About