Antibacterial activity of polyphenol-rich pomegranate peel extract against
            <i>Cronobacter sakazakii</i>

Yemiş, Gökçe Polat; Bach, Susan; Delaquis, Pascal

doi:10.1080/10942912.2019.1622564

Cited by 23 publications

(8 citation statements)

References 36 publications

(45 reference statements)

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“…% (Figure 4). These results are clearly indicative of the high antimicrobial performance of pomegranate seed and peel extracts, as reported by several authors [57,58]. This behaviour can be related to the polyphenolic compounds present in PSP in accordance with previous results linking the antibacterial activity of pomegranate fruit and peel extracts with their total polyphenols content [59][60][61], being phenols, tannins and flavonoids the major active compounds responsible for this activity.…”

Section: Antimicrobial Activity Of Fg/psp Filmssupporting

confidence: 91%

Gelatin-Based Antimicrobial Films Incorporating Pomegranate (Punica granatum L.) Seed Juice by-Product

et al. 2019

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Pomegranate (Punica granatum L.) seed juice by-product (PSP) was added as reinforcing and antimicrobial agent to fish gelatin (FG) films as a promising eco-friendly active material for food packaging applications. A complete linkage analysis of polysaccharides in PSP showed xylan and cellulose as main components. This residue showed also high total phenolic content and antioxidant activity. Three formulations were processed by adding PSP to FG (0, 10, 30 wt. %) by the casting technique, showing films with 10 wt. % of PSP the best performance. The addition of PSP decreased elongation at break and increased stiffness in the FG films, particularly for 30 wt. % loading. A good compatibility between FG and PSP was observed by SEM. No significant (p < 0.05) differences were obtained for barrier properties to oxygen and water vapour permeability compared to the control with the incorporation of PSP, whereas water resistance considerably increased and transparency values decreased (p < 0.05). High thermal stability of films and inhibition against S. aureus were observed. The addition of PSP at 10 wt. % into FG was shown as a potential strategy to maintain the integrity of the material and protect food against lipid oxidation, reducing huge amounts of pomegranate and fish wastes.

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Section: Antimicrobial Activity Of Fg/psp Filmssupporting

confidence: 91%

Gelatin-Based Antimicrobial Films Incorporating Pomegranate (Punica granatum L.) Seed Juice by-Product

et al. 2019

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“…Among these, plant crude extracts were more suitable as the natural disinfectants due to their low cost and simple processing technique. Yemis et al (2019) found that the MIC of polyphenol-rich pomegranate peel extract against C. sakazakii was 20 mg/mL. Fei et al (2020) explored the possibility of Amaranthus tricolor crude extract (ATCE) as a potential disinfectant and found that the MIC and MBC of ATCE against C. sakazakii were 20 and 40 mg/mL, respectively.…”

Section: Discussionmentioning

confidence: 99%

Antibacterial Activity of Chrysanthemum buds Crude Extract Against Cronobacter sakazakii and Its Application as a Natural Disinfectant

Chang

Xing

et al. 2021

Front. Microbiol.

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Cronobacter sakazakii is an opportunistic food-borne pathogen that endangers the health of neonates and infants. This study aims to elucidate the antibacterial activity and mechanism of Chrysanthemum buds crude extract (CBCE) against C. sakazakii and its application as a natural disinfectant. The antibacterial activity was evaluated by the determination of the diameter of inhibition zone (DIZ), minimum inhibitory concentration (MIC), and minimum bactericide concentration (MBC). The antibacterial mechanism was explored based on the changes of growth curve assay, intracellular ATP concentration, membrane potential, intracellular pH (pHin), content of soluble protein and nucleic acid, and cell morphology. Finally, the inactivation effects of CBCE against C. sakazakii in biofilm on stainless steel tube, tinplate, glass, and polystyrene were evaluated. The results showed that the DIZ, MIC, and MBC of CBCE against C. sakazakii were 14.55 ± 0.44–14.84 ± 0.38 mm, 10 mg/mL, and 20 mg/mL, respectively. In the process of CBCE acting on C. sakazakii, the logarithmic growth phase of the tested bacteria disappeared, and the concentrations of intracellular ATP, pHin, bacterial protein, and nucleic acid were reduced. Meanwhile, CBCE caused the cell membrane depolarization and leakage of cytoplasm of C. sakazakii. In addition, about 6.5 log CFU/mL of viable C. sakazakii in biofilm on stainless steel tube, tinplate, glass, and polystyrene could be inactivated after treatment with 1 MIC of CBCE for 30 min at 25°C. These findings reveal the antibacterial activity and mechanism of CBCE against C. sakazakii and provide a possibility of using a natural disinfectant to kill C. sakazakii in the production environment, packaging materials, and utensils.

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“…The MIC of ATCE against C. sakazakii strains was 20 mg/mL, a value higher than that of some other pure natural products, such as eugenol (MIC = 1.0 mg/mL), lipoic acid (MIC = 2.5-5 mg/mL), and syringic acid (MIC = 5.0 mg/mL) (Bajpai et al, 2013;Shi et al, 2016b,c), which indicated that further concentrations of ATCE should be evaluated to achieve a better bacteriostatic effect. Similarly, the average MIC of polyphenol-rich pomegranate peel extract (pH = 6.0) against C. sakazakii was 20 mg/mL, according to the report of Yemis et al (2019), who deemed that a MIC of 20 mg/mL was acceptable for the crude extract, and that a concentration process be performed before using the extract as a disinfectant.…”

Section: Discussionmentioning

confidence: 99%

Amaranthus tricolor crude extract inhibits Cronobacter sakazakii isolated from powdered infant formula

Peng

Feng

Wang

et al. 2020

Journal of Dairy Science

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The purpose of this study was to elucidate the antibacterial activity and possible mechanism of action of Amaranthus tricolor crude extract (ATCE) against Cronobacter sakazakii isolated from powdered infant formula (PIF). The antibacterial activity of ATCE was assessed by measuring the diameter of inhibition zone (DIZ), minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC). The possible mechanism of action of ATCE was revealed by analyzing the effects of ATCE on growth curves and changes in cell membrane potential, intracellular pH, content of bacterial protein and genomic DNA, and cell morphology. Finally, ATCE was applied to the disinfection of C. sakazakii in biofilm on stainless steel tube. The results showed that the DIZ, MIC, and MBC of ATCE against C. sakazakii strains were from 14.35 ± 0.67 to 14.84 ± 0.67 mm, 20 mg/mL, and 40 mg/mL, respectively. Treatment with ATCE ended the logarithmic growth phase of C. sakazakii, and led to depolarization of the cell membranes, reducing intracellular pH and bacterial protein and genomic DNA contents, and resulting in cytoplasmic leakage and deformation. In addition, ATCE effectively inactivated C. sakazakii in biofilm, reducing viable bacteria by approximately 6.5 log cfu/mL bacterial count after treatment with 1 MIC (1 MIC = 20 mg/mL) of ATCE for 20 min at 25°C. Our findings showed that ATCE inactivated C. sakazakii strains isolated from PIF and has potential as a natural disinfectant to reduce the contamination of PIF by C. sakazakii.

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Antibacterial activity of polyphenol-rich pomegranate peel extract against Cronobacter sakazakii

Cited by 23 publications

References 36 publications

Gelatin-Based Antimicrobial Films Incorporating Pomegranate (Punica granatum L.) Seed Juice by-Product

Gelatin-Based Antimicrobial Films Incorporating Pomegranate (Punica granatum L.) Seed Juice by-Product

Antibacterial Activity of Chrysanthemum buds Crude Extract Against Cronobacter sakazakii and Its Application as a Natural Disinfectant

Amaranthus tricolor crude extract inhibits Cronobacter sakazakii isolated from powdered infant formula

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