Antibacterial activity of extracellular compounds produced by a Pseudomonas strain against methicillin-resistant Staphylococcus aureus (MRSA) strains

Abstract

BackgroundThe emergence of multidrug-resistant bacteria is a world health problem. Staphylococcus aureus, including methicillin-resistant S. aureus (MRSA) strains, is one of the most important human pathogens associated with hospital and community-acquired infections. The aim of this work was to evaluate the antibacterial activity of a Pseudomonas aeruginosa-derived compound against MRSA strains.MethodsThirty clinical MRSA strains were isolated, and three standard MRSA strains were evaluated. The extracellular compounds were purified by vacuum liquid chromatography. Evaluation of antibacterial activity was performed by agar diffusion technique, determination of the minimal inhibitory concentration, curve of growth and viability and scanning electron microscopy. Interaction of an extracellular compound with silver nanoparticle was studied to evaluate antibacterial effect.ResultsThe F3 (ethyl acetate) and F3d (dichloromethane- ethyl acetate) fractions demonstrated antibacterial activity against the MRSA strains. Phenazine-1-carboxamide was identified and purified from the F3d fraction and demonstrated slight antibacterial activity against MRSA, and synergic effect when combined with silver nanoparticles produced by Fusarium oxysporum. Organohalogen compound was purified from this fraction showing high antibacterial effect. Using scanning electron microscopy, we show that the F3d fraction caused morphological changes to the cell wall of the MRSA strains.ConclusionsThese results suggest that P. aeruginosa-produced compounds such as phenazines have inhibitory effects against MRSA and may be a good alternative treatment to control infections caused by MRSA.

Activity of Secondary Bacterial Metabolites in the Control of Citrus Canker

“…In the previous studies was testing the effect of secondary metabolite produced by Pseudomonas aeruginosa strain LV on different phytopathogenic bacteria [8]- [10], and other pathogenic bacteria [18] [19]. In the present study was evaluate the effect secondary metabolites produced by Pseudomonas sp.…”

Anti-methicillin Resistant Staphylococcus aureus Compound Isolation from Halophilic Bacillus amyloliquefaciens MHB1 and Determination of Its Mode of Action Using Electron Microscope and Flow Cytometry Analysis

“…Among them, MRSA has been considered as an important nosocomial pathogen among the human population [1,2]. The thick cell wall of S. aureus confers resistance towards all b-lactam antibiotics, including methicillin, oxacillin, penicillin and amoxicillin [25].…”

“…The optimized C/N ratio of 30.76, temperature at 32°C, pH at 8.0 and NaCl at 12 % in the ZMB medium enhanced the anti-MRSA activity. Further, extraction and chromatographic purification steps resulted in the isolation of purified compound which belonged to the phenolic group based on TLC, LC-MS, 1 H NMR and FTIR analysis. Earlier studies revealed that this phenolic group of compounds exhibited antagonistic activity against MRSA.…”

“…Over the last few decades, the emergence of multidrug resistant pathogens imposes many health related problems in humans worldwide [1]. Among them, methicillin-resistant Staphylococcus aureus (MRSA) is an important nosocomial pathogen and has been associated with the major cause of skin and soft tissue infections [2].…”

Antibacterial Activity of Three Medicinal Lasianthus (Rubiaceae) Extracts on Human Resistant Pathogenic Bacteria

“…The bacteria on the filter paper were fixed by a 2.5% glutaraldehyde in 0.2 M sodium phosphate saline buffer (pH 7.2) for 12 hours at 4ºC. The fixed bacteria were washed three times with 0.2 M sodium phosphate saline buffer (pH 7.2) and were then post-fixed in 1% osmium tetroxide (OsO 4 ) in distilled water for one hour [14]. After that, the filter paper was cleaned up with three cycles of distilled water.…”