Antiapoptotic and neuroprotective effects of mycophenolate mofetil after acute spinal cord injury in young rats

Bilginer, Burçak; Önal, Mehmet Bülent; Narin, Fırat; Üstün, Hüseyin; Kılınç, Kamer; Akalan, Nejat

doi:10.1007/s00381-009-0985-5

Cited by 11 publications

(7 citation statements)

References 25 publications

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“…The observed neuroprotective effect of mycophenolate could be partially attributed to its anti-oxidant activity, as mycophenolate treatment at doses of 100 and 200 mg/kg ameliorated the raised NO activity after 24 h of ischemia (Bilginer et al, 2009). Our results demonstrated that cephalexin, mycophenolate, and atorvastatin reduced the expression of proinflammatory cytokines and oxidative stress-related factors like TNF-α, GFAP, and COX-2.…”

Section: Discussionsupporting

confidence: 52%

RETRACTED: NF-κB Inhibitors Attenuate MCAO Induced Neurodegeneration and Oxidative Stress—A Reprofiling Approach

Ali

Shah

Zeb

et al. 2020

Front. Mol. Neurosci.

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Stroke is the leading cause of morbidity and mortality worldwide. About 87% of stroke cases are ischemic, which disrupt the physiological activity of the brain, thus leading to a series of complex pathophysiological events. Despite decades of research on neuroprotectants to probe for suitable therapies against ischemic stroke, no successful results have been obtained, and new alternative approaches are urgently required in order to combat this pathological torment. To address these problems, drug repositioning/reprofiling is explored extensively. Drug repurposing aims to identify new uses for already established drugs, and this makes it an attractive commercial strategy. Nuclear factor-kappa beta (NF-κB) is reported to be involved in many physiological and pathological conditions, such as neurodegeneration, neuroinflammation, and ischemia/reperfusion (I/R) injury. In this study, we examined the neuroprotective effects of atorvastatin, cephalexin, and mycophenolate against the NF-κB in ischemic stroke, as compared to the standard NF-κB inhibitor caeffic acid phenethyl ester (CAPE). An in-silico docking analysis was performed and their potential neuroprotective activities in the in vivo transient middle cerebral artery occlusion (t-MCAO) rat model was examined. The percent (%) infarct area and 28-point composite neuro score were examined, and an immunohistochemical analysis (IHC) and enzyme-linked immunosorbent assay (ELISA) were further performed to validate the neuroprotective role of these compounds in stroke as well as their potential as antioxidants. Our results demonstrated that these novels NF-κB inhibitors could attenuate ischemic stroke-induced neuronal toxicity by targeting NF-κB, a potential therapeutic approach in ischemic stroke.

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Section: Discussionsupporting

confidence: 52%

RETRACTED: NF-κB Inhibitors Attenuate MCAO Induced Neurodegeneration and Oxidative Stress—A Reprofiling Approach

Ali

Shah

Zeb

et al. 2020

Front. Mol. Neurosci.

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“…Weight-drop model was performed for SCI. [4,20,21] The animals were subjected to an impact of 50 g/cm to the dorsal surface of the spinal cord. The force was applied via a stainless steel rod (3 mm diameter tip, weight 5 g) that was rounded at the surface.…”

Section: Methodsmentioning

confidence: 99%

Antioxidant effects of curcumin in spinal cord injury in rats

Kavakli

Koca²,

Alıcı³

2011

Ulus Travma Acil Cerrahi Derg

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AMAÇBu deneysel çalışma, sıçanlarda spinal kord yaralanmasın-da curcuminin antioksidan etki yoluyla faydasını araştır-mak için yapıldı. GEREÇ VE YÖNTEMYirmi dört adet Wistar albino sıçan 3 gruba randomize edildi. Spinal kord yaralanması ağırlık düşürme modeliyle gerçekleştirildi. Grup 1'e laminektomi ardından spinal kord yaralanması uygulandı ve herhangi bir tedavi verilmedi. Grup 2'ye laminektomi ardından spinal kord yaralanması uygulandı ve curcumin verildi (200 mg/kg/gün ağızdan). Grup 3'e laminektomi ardından spinal kord yaralanması uygulandı ve metilprednizolon verildi (30 mg/kg periton içine), 24 saat sonra tüm sıçanlardan kan örnekleri alındı, sonra serum süperoksit dismutaz (SOD) ve malondialdehit (MDA) düzeyleri belirlendi ve elde edilen sonuçlar karşılaştırıldı. BULGULARCurcumin grubunda SOD düzeyi kontrol ve metilprednizolon grubundan daha yüksekti (p<0,001 ve p<0,012). Curcumin grubunda MDA düzeyi kontrol grubundan daha düşüktü (p<0,042). Benzer şekilde metilprednizolon grubunda MDA düzeyi kontrol grubundan daha düşüktü (p<0,001). SONUÇBu çalışmanın sonuçları curcuminin etkin biçimde oksidatif hasara karşı spinal kord dokularını koruduğunu gös-terir.Anahtar Sözcükler: Antioksidan; curcumin; malondialdehit; spinal kord yaralanması; süperoksit dismutaz.

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“…Several chemicals, ranging from immunosuppressive to anti-inflammatory agents, have been studied as therapeutic agents. [ 11 , 12 ] Methylprednisolone, a strong anti-inflammatory agent that was once thought to be effective and recommended in guidelines, was later found to be ineffective in subsequent studies. Considering the severe complications associated with high-dose steroids, their use has been widely abandoned.…”

Section: Discussionmentioning

confidence: 99%

“…This activation results in an excessive release of Ca 2+ under pathological conditions, which, in turn, may progress to cellular death. [ 12 ] The extent of axonal damage has been shown to be limited by chelating intracellular Ca 2+ . [ 13 ] It is thought that the endoplasmic reticulum (ER) is the source of intracellular Ca 2+ during injury.…”

Section: Discussionmentioning

confidence: 99%

Effects of lercanidipine on traumatic spinal cord injury: an experimental study

Türk

2024

Ulus Travma Acil Cerrahi Derg

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BACKGROUND: Spinal cord injury is a devastating trauma that leaves survivors at risk for several medical complications throughout their lives. Lercanidipine, a third-generation calcium channel blocker, possesses anti-apoptotic, anti-inflammatory, and antioxidative properties. This study aimed to evaluate the neuroprotective effects of lercanidipine in an experimental spinal cord trauma model. METHODS: Twenty-one Wistar rats were randomly assigned to three groups. Group 1 (G1) underwent laminectomy. Group 2 (G2) were subjected to trauma following laminectomy. Group 3 (G3) were exposed to trauma following laminectomy and treated with lercanidipine. Lercanidipine was administered intraperitoneally for seven days. Histopathological and immunohistochemical evaluations were conducted. RESULTS: Regarding Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining, there was no significant difference among the groups. However, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) levels were significantly different across the groups. G2 had significantly higher NF-κB levels compared to G1 and G3. CONCLUSION: Lercanidipine, a third-generation calcium channel blocker, is effective against inflammatory responses induced in spinal cord injury. Further studies are required to determine its capability in preventing apoptosis or improving functional recovery. To the best of our knowledge, this study is the first in the literature to examine the neuroprotective effects of lercanidipine on spinal cord injury.

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Antiapoptotic and neuroprotective effects of mycophenolate mofetil after acute spinal cord injury in young rats

Abstract: Our results showed that the administration of mycophenolate mofetil on traumatic spinal cord injury decreases apoptosis and improves neurologic recovery.

Cited by 11 publications

References 25 publications

RETRACTED: NF-κB Inhibitors Attenuate MCAO Induced Neurodegeneration and Oxidative Stress—A Reprofiling Approach

RETRACTED: NF-κB Inhibitors Attenuate MCAO Induced Neurodegeneration and Oxidative Stress—A Reprofiling Approach

Antioxidant effects of curcumin in spinal cord injury in rats

Effects of lercanidipine on traumatic spinal cord injury: an experimental study

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