Antiangiogenic property of pigment epithelium-derived factor in hepatocellular carcinoma

Matsumoto, Kojiro; Ishikawa, Hiroki; Nishimura, Daisuke; Hamasaki, Koji; Nakao, Kazuwa; Eguchi, Katsumi

doi:10.1002/hep.20259

Cited by 106 publications

(89 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The proteomic screen also showed over-expression of novel proteins such as the 14-3-3 proteins which are phospho-serine/phosphothreonine-binding proteins that may promote cell survival and have been suggested to be a possible target for therapies aimed at enhancing killing of cancer cells [20]. Other proteins that were overexpressed and could be postulated to have a link to tumorigenesis or cancer growth or spread included pigment epithelium derived factor precursor, a potent anti-angiogenic protein that has the potential to limit tumor growth [21]. Interestingly, we also found increased expression of periostin in the tumoral liver tissue compared to non-tumoral liver tissue and this protein has been described in other tumor types for example breast cancers, melanoma and colon cancers ( [22][23][24]).…”

Section: Proteins Under-expressed In the Tumoral Liver Tissue Comparementioning

confidence: 99%

Proteomic Analysis of Differentially Expressed Proteins in Peripheral Cholangiocarcinoma

Darby

Vuillier-Devillers

Pinault

et al. 2010

Cancer Microenvironment

View full text Add to dashboard Cite

Cholangiocarcinoma is an adenocarcinoma of the liver which has increased in incidence over the last thirty years to reach similar levels to other liver cancers. Diagnosis of this disease is usually late and prognosis is poor, therefore it is of great importance to identify novel candidate markers and potential early indicators of this disease as well as molecules that may be potential therapeutic targets. We have used a proteomic approach to identify differentially expressed proteins in peripheral cholangiocarcinoma cases and compared expression with paired non-tumoral liver tissue from the same patients. Two-dimensional fluorescence difference gel electrophoresis after labeling of the proteins with cyanines 3 and 5 was used to identify differentially expressed proteins. Overall, of the approximately 2,400 protein spots visualised in each gel, 172 protein spots showed significant differences in expression level between tumoral and non-tumoral tissue with p<0.01. Of these, 100 spots corresponding to 138 different proteins were identified by mass spectrometry: 70 proteins were over-expressed whereas 68 proteins were under-expressed in tumoral samples compared to nontumoral samples. Among the over-expressed proteins, immunohistochemistry studies confirmed an increased expression of 14-3-3 protein in tumoral cells while α-smooth muscle actin and periostin were shown to be overexpressed in the stromal myofibroblasts surrounding tumoral cells. α-Smooth muscle actin is a marker of myofibroblast differentiation and has been found to be a Ian A. Darby, Karine Vuillier-Devillers, and Émilie Pinault have equally contributed to this work.

show abstract

Section: Proteins Under-expressed In the Tumoral Liver Tissue Comparementioning

confidence: 99%

Proteomic Analysis of Differentially Expressed Proteins in Peripheral Cholangiocarcinoma

Darby

Vuillier-Devillers

Pinault

et al. 2010

Cancer Microenvironment

View full text Add to dashboard Cite

show abstract

“…These results suggest that a sufficient bystander effect was achieved by this strategy, and if the transgene is expressed intratumorally, highly efficient therapeutic gene induction may not be necessarily required. We have reported that PEDF gene transduction into Huh-7 tumor significantly repressed its growth in athymic mouse models as well as tum-1 shown in this study, but PEDF did not directly inhibit the proliferation of Huh-7 cells in vitro (8). Therefore, it is likely that antitumor effect of tum-1 in vivo could be attributable to its anti- angiogenic activity rather than direct antiproliferative activity against Huh-7 cells.…”

Section: Discussionmentioning

confidence: 53%

“…PLC/PRF/5 cells stably transfected with pSecTag2B-tum-1 or vehicle were plated on 100-mm dishes. After 24 h, the medium was replaced with 5 ml serum-free RPMI and incubated for 48 h. (7,8). Briefly, 600 μl of CM-Mock or CM-tum-1 with or without 10 ng/ml of VEGF was placed in the lower chamber.…”

Section: Methodsmentioning

confidence: 99%

“…Therefore, it is conceivable that antiangiogenic gene delivery to cancer cells could be suitable for the treatment of HCC, where it can increase the local concentration of therapeutic endogenous agents. In fact, we have reported that the introduction of antiangiogenic genes such as angiostatin and pigment epithelium-derived factor (PEDF) genes into HCC cells exhibited significant antitumor effect in xenograft models (7,8).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Tum-1, a tumstatin fragment, gene delivery into hepatocellular carcinoma suppresses tumor growth through inhibiting angiogenesis

Goto

Ishikawa²,

Matsumoto³

et al. 2008

Int J Oncol

Self Cite

View full text Add to dashboard Cite

Abstract. Since hepatocellular carcinoma (HCC) is a hypervascular cancer, anti-angiogenic therapy is a promising approach to treat HCC. In the present study, we investigated the antiangiogenic and antitumor effects of tum-1, a fragment of tumstatin, gene transduction into HCC in vitro and in vivo. Tum-1 gene was cloned into a pSecTag2B mammalian expression vehicle to construct pSecTag2B-tum-1. pSecTag2B-tum-1 or vehicle were transfected into human HCC cells, PLC/PRF/5 cells stably and Huh-7 cells transiently. pSecTag2B-tum-1 transfection slightly repressed the proliferation of both PLC/PRF/5 and Huh-7 cells in vitro. Addition of conditioned media (CM) from tum-1 expressing PLC/PRF/5 cells significantly inhibited the spontaneous and vascular endothelial growth factor (VEGF)-induced proliferation and migration of human umbilical vein endothelial cells (HUVEC) in vitro with diminishing the VEGF-induced phosphorylation of both Akt and extracellular signal-regulated kinase (ERK) that are known to mediate VEGF-induced proliferation and migration of endothelial cells. In in vivo experiments, intratumoral injection of pSecTag2B-tum-1 significantly repressed the growth of pre-established Huh-7 tumors in athymic mouse models accompanying the decreased density of CD34 positive vessels in tumors. In conclusion, our results suggest that antiangiogenic gene therapy using tum-1 gene may be an efficient strategy for the treatment of HCC.

show abstract

“…Researchers did a number of studies and showed that there is opposite relation between PEDF levels, grade and metastatic potential of prostate tumors (Halin et al, 2004), pancreatic adenocarcinoma (Uehara et al, 2004), prostate, melanoma, ovarian, OSA, glioma (Murray et al, 2010), hepatocellular carcinoma (Matsumoto et al, 2004) …”

Section: The Role Of Pedf In the Prevention Of The Osamentioning

confidence: 99%

Review of the Molecular Pathogenesis of Osteosarcoma

Hao²,

Wang³

et al. 2014

Asian Pacific Journal of Cancer Prevention

124

109

View full text Add to dashboard Cite

Treating the osteosarcoma (OSA) remains a challenge. Current strategies focus on the primary tumor and have limited efficacy for metastatic OSA. A better understanding of the OSA pathogenesis may provide a rational basis for innovative treatment strategies especially for metastases. The aim of this review is to give an overview of the molecular mechanisms of OSA tumorigenesis, OSA cell proliferation, apoptosis, migration, and chemotherapy resistance, and how improved understanding might contribute to designing a better treatment target for OSA.

show abstract

Antiangiogenic property of pigment epithelium-derived factor in hepatocellular carcinoma

Cited by 106 publications

References 34 publications

Proteomic Analysis of Differentially Expressed Proteins in Peripheral Cholangiocarcinoma

Proteomic Analysis of Differentially Expressed Proteins in Peripheral Cholangiocarcinoma

Tum-1, a tumstatin fragment, gene delivery into hepatocellular carcinoma suppresses tumor growth through inhibiting angiogenesis

Review of the Molecular Pathogenesis of Osteosarcoma

Contact Info

Product

Resources

About