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Cited by 36 publications
(53 citation statements)
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References 25 publications
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“…The correct in-frame cloning of E7, E6, and E5-encoding genes was confirmed by nucleotide sequencing. The DNA vaccine (pgD) encoding the complete nonfused HSV-1 gD has been described previously (24).…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…The correct in-frame cloning of E7, E6, and E5-encoding genes was confirmed by nucleotide sequencing. The DNA vaccine (pgD) encoding the complete nonfused HSV-1 gD has been described previously (24).…”
Section: Methodsmentioning
confidence: 99%
“…Improved activation of antigen-specific CD8 ϩ T-cell responses by anti-HPV DNA vaccines were achieved after genetic fusion of the E7 or E6 oncoprotein with different carrier proteins carrying cell targeting signals or mediators of immune responses (8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19). The focus of our DNA vaccines targeting HPV-induced cancers has been the augmentation of adaptive immune responses through the blockade of an immune inhibitory pathway based on the expression of hybrid proteins genetically fused with glycoprotein D (gD) of herpes simplex virus (HSV) (24,25). HSV gD binds the herpes virus entry mediator (HVEM) and competes for the same binding site as the B-and T-lymphocyte attenuator (BTLA).…”
mentioning
confidence: 99%
“…The DNA vaccine encoding the HPV-16 E7 protein genetically fused after amino acid 244 of the HSV-1 gD protein has been described previously (21,23). The gene sequence encoding the gDE7 chimeric protein was cloned into the pVAX1 vector (Invitrogen), which contains a cytomegalovirus (CMV) promoter and a kanamycin resistance gene.…”
Section: Dna Vaccinesmentioning
confidence: 99%
“…We showed previously that a vaccine expressing an Ag fused into the C-terminal domain of gD away from the HVEM binding N terminus induces a more potent immune response in mice than the same vaccine expressing the Ag without gD (36). In this study, we tested if this strategy can augment a CD8 + T cell response to influenza Avirus in aged mice immunized with a replication-defective adenovirus (Ad) vector derived from chimpanzee serotype 68 (AdC68).…”
Section: Foxp3mentioning
confidence: 99%