Anti sickling potential and chemical profiling of traditionally used Woodfordia fruticosa (L.) Kurz leaves

Mishra, Shringika; Sonter, Shruti; Dwivedi, Manish Kumar; Singh, Prashant

doi:10.1016/j.arabjc.2021.103539

Cited by 7 publications

(5 citation statements)

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“…It is noted that 200 mg/kg bw did not show a significant effect because the lesser quantity might not possess enough aptitude to lessen diarrhea 67 . The antidiarrheal potentials of the plant might be characterized by the presence of flavonoids, tannins, sterols, triterpenes, and so on, which might have possessed the antagonistic ability by slowing down gastrointestinal motility and emission caused by castor oil via inhibiting the secretion of autacoids and prostaglandins 7,68–70 . These plant extracts rich in flavonoids may be an effective treatment for both acute and chronic diarrhea because of their capacity to reduce intestinal fluid output and slow bowel motions.…”

Section: Discussionmentioning

confidence: 99%

“… 67 The antidiarrheal potentials of the plant might be characterized by the presence of flavonoids, tannins, sterols, triterpenes, and so on, which might have possessed the antagonistic ability by slowing down gastrointestinal motility and emission caused by castor oil via inhibiting the secretion of autacoids and prostaglandins. 7 , 68 , 69 , 70 These plant extracts rich in flavonoids may be an effective treatment for both acute and chronic diarrhea because of their capacity to reduce intestinal fluid output and slow bowel motions. Additionally, they might protect the intestines from oxidative stress and preserve the mucosal lining's integrity, potentially minimizing the severity of long‐term inflammatory damage.…”

Section: Discussionmentioning

confidence: 99%

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Exploring therapeutic potential of Woodfordia fruticosa (L.) Kurz leaf and bark focusing on antioxidant, antithrombotic, antimicrobial, anti‐inflammatory, analgesic, and antidiarrheal properties

Rahman,

Soma,

Sultana

et al. 2023

Health Science Reports

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Background and AimsThe study aimed to evaluate the pharmacological properties of methanolic extracts of leaves and barks of Woodfordia fruticosa (L.) Kurz (family: Lythraceae) focusing on antioxidant, thrombolytic, anti‐inflammatory, antibacterial, analgesic, and antidiarrheal effects.Methods1,1‐Diphenyl‐2‐picrylhydrazyl (DPPH) free radical scavenging assay, clot lysis, disc diffusion, and membrane stabilizing methods were employed to assess in vitro antioxidant, thrombolytic, antibacterial, and anti‐inflammatory properties of the leaf and bark methanolic extracts (ME) of W. fruticosa and different organic solvents, that is, petroleum ether (PE), dichloromethane (DCM), chloroform (CL), and aqueous (AQ) fractions. In addition, in vivo central and peripheral analgesic and antidiarrheal activities of both crude extracts were evaluated at two doses (200 and 400 mg/kg of body weight [bw]).ResultsAll the extracts and fractions showed promising antioxidant properties by scavenging DDPH free radicals with IC50 of 6.11–20.79 μg/mL. AQ fraction (41.24%) of leaves and ME (44.90%) of bark exerted notable in vitro thrombolytic activity. The CL fraction of leaves and AQ fraction of the bark showed 43.16% and 45.37% inhibition of RBC hemolysis, respectively, compared to the inhibition of RBC hemolysis by aspirin in a hypotonic‐induced membrane stabilizing assay. Besides, both extracts were observed to provide significant (p < 0.001) central and peripheral analgesic responses at both doses of 200 and 400 mg/kg bw. Furthermore, both doses of bark extract (p < 0.001) and the 400 mg/kg bw of leaf extract (p < 0.05) were observed to possess statistically significant antidiarrheal activity. Additionally, in an in vivo acute toxicity investigation, both extracts had a median lethal dose (LD50) greater than 5000 mg/kg bw, indicating their safety level.ConclusionThe current study proves the ethnomedicinal uses of W. fruticosa; however, further studies are required for phytochemical screening to isolate the responsible bioactive compounds and discover the lead molecules from the plant species.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Exploring therapeutic potential of Woodfordia fruticosa (L.) Kurz leaf and bark focusing on antioxidant, antithrombotic, antimicrobial, anti‐inflammatory, analgesic, and antidiarrheal properties

Rahman,

Soma,

Sultana

et al. 2023

Health Science Reports

View full text Add to dashboard Cite

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“…Several plant extracts have demonstrated antisickling properties through the inhibitory efect of hemoglobin S polymerization, the reversibility of sickling, or the increase of the Fe 2+ /Fe 3+ ratio. Tese include the hydroethanolic extract of Ficus sycomorus leaves [9], aqueous extract of Hemodya [10], aqueous extracts of Zanthoxyllum heitzii [11], hydroethanolic extract of Teobroma cacao [12], aqueous extracts of Phaseolus vulgarus L. [13] and Rubia cordifolia L. [14], leaves of Rhaphiostylis beninensis [15], and Woodfordia fruticosa (L.) Kurz leaves [16], among others. Other studies demonstrated that nutraceutic and nutrition supplement have benefcial efects on the management of sickle cell diseases [17,18].…”

Section: Introductionmentioning

confidence: 99%

“…Other studies demonstrated that nutraceutic and nutrition supplement have benefcial efects on the management of sickle cell diseases [17,18]. Te benefcial efects of these nutraceutic and nutrition in the management of sickle cell disease are attributed both to their higher levels of micronutrients (vitamins and minerals) and bioactive molecules (phenolic compounds and amino acids) composition, as well as the antiradical and antioxidant properties of these bioactive compounds [13][14][15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Antisickling and Antihemolytic Mechanism of Spirulina platensis (Oscillatoriaceae): A Nutraceutical Commonly Used in Cameroon

Teguem Tchoulegheu,

Nya Nkwikeu,

Lena Yembeau

et al. 2023

Evidence-Based Complementary and Alternative Medicine

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Sickle cell anaemia (SCA) is a widespread genetic disease in Africa, associated with chronic hemolytic anaemia and vaso-occlusive and infectious complications. The most commonly used means of management and treatment such as blood transfusions and allografting are expensive and predispose patients to the risk of infections. This research study aimed at evaluating the antisickling and antihemolytic activities of aqueous extracts of Spirulina platensis from Cameroon for optimising the management of this disease using natural substances. The Spirulina platensis harvested in Nomayos-Yaounde was dried, crushed, and macerated for 24 h in distilled water and the filtrate was freeze dried. The determination of the inhibition rates of falciformation induced by 2% sodium metabisulfite (MBS) and the sickling reversibility rate was carried out at different concentrations (100, 200, 400, 800, and 1600 μg·mL−1) of the spirulina extract at different time intervals (2 h, 4 h, and 24 h). Aspirin, hypotonic solution, Triton X-100, and hydrogen peroxide were used as hemolytic inducers and the antihemolytic activity of the extract was studied at 800 μg·mL−1 and 1600 μg·mL−1 using the colorimetric method. The extraction yield was 14.015%. The maximum duration of induced falciformation was 2 h 30 min and the percentage of falciformation increased from 27.99 ± 3.15% (at the initial time) to 91.44 ± 3.70%, giving a falciformation induction rate of 69.3%. The falciformation inhibition rate after 2 h 30 min ranges from 15.10 ± 0.60% to 66.09 ± 4.69% for the concentration of 100 μg·mL−1 to 1600 μg·mL−1 of the spirulina extract. This rate of inhibition of falciformation was found to be dose-dependent. The best concentration of the extract was 1600 μg·mL−1. The reversibility rate of falciformation at 800 μg·mL−1 and 1600 μg·mL−1 varied from 37.54 ± 6.35% to 82.34 ± 5.63% as a function of time. 1600 μg·mL−1 was the most active concentration after 24 h. In addition, the extract improved the Fe2+/Fe3+ ratio with an increase in the rates of 69.78 ± 8.81 and 69.78 ± 13.82 at 800 μg·mL−1 and 1600 μg·mL−1, respectively. According to each inducer at 800 μg·mL−1 and 1600 μg·mL−1, respectively, of the spirulina extract, the following rates of inhibition of hemolysis were found: 53.03 ± 9.46% and 96.67 ± 5.77% (aspirin); 80 ± 8.66% and 71.25% (hypotonic solution); 36.56 ± 9.53% and 45.67 ± 22.55% (Triton X-100); 24.26 ± 9.55% and 36.76 ± 1.27% (hydrogen peroxide). At the end of this study, the best antickling activities were obtained at the concentrations 800 μg·mL−1 and 1600 μg·mL−1. It also has antihemolytic properties on various hemolysis inducers at concentrations 800 μg·mL−1 and 1600 μg·mL−1 with inhibition rates varying from 36% to 96%.

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“…Myricetin, oenothein B, isochimacoalin-A, –terpinene, limonene, and oligomers such as woodfordins A, B, C, E, G, H, and I, as well as quercetin, are among the phenolic compounds found in the flowers. The anti-inflammatory activities of quercetin and myricetin are related to their inhibition of the two key enzymes, lipoxygenase and cyclooxygenase of pelargonidin, that play a key role in the pathophysiology of inflammation ( 1 , 4 ). –terpinene and limonene have antibacterial and antifungal characteristics, and they act by entering the cell membrane and destroying cellular components ( 5 , 6 ).…”

Section: Introductionmentioning

confidence: 99%

Woodfordia fruticosa extract nanoemulsion: Influence of processing treatment on droplet size and its assessment for in vitro antimicrobial and anti-inflammatory activity

et al. 2022

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Recently, plant-derived bioactive compounds have been utilized in the preparation of several functional food products; however, stability and water solubility are major constraints to these compounds. Therefore, to overcome this problem, the synthesis of nanoemulsion (oil in water) with varying concentrations of Woodfordia fruticosa flower extract (1%−10% w/v) was carried out and characterization of nanoemulsion was done. The average droplet size of nanoemulsion samples ranges from 149.25 to 244.33 nm. The control and WFNE3 nanoemulsion showed significantly (p < 0.05) higher thermal stability when correlated with average droplet size. An insignificant difference (p > 0.05) was observed in the average droplet size and zeta potential WFNE3 (−30.3mV) with the increased temperature rate. At varied pH ranges, WFNE3 showed significantly higher (p < 0.05) stability in comparison with the control nanoemulsion sample. In terms of ionic strength, WFNE3 nanoemulsion sample showed significantly (p < 0.05) higher stability, and with an increasing concentration of salt, the colloidal system of the WFNE3 sample showed significantly (p < 0.05) higher droplet size (318.91 nm). Therefore, the antimicrobial potential of WFNE3 nanoemulsion in comparison with extract of W. fruticosa flower extract was studied against Gram-positive Staphylococcus aureus, Gram-negative bacteria Pseudomonas aeruginosa, and fungal strain Candida albicans, respectively. WFNE3 nanoemulsion sample in comparison to flower extract showed a significantly higher (p < 0.05) zone of inhibition against gram-negative bacteria as compared to the control nanoemulsion sample upon storage for 7 days. WFNE3 nanoemulsion sample showed significant (p < 0.05) higher inhibition of protein denaturation (57.89%−87.65%) and (55.36%−83.58%) in comparison to control nanoemulsion sample (54.67%−80.28%) and flower extract (51.56%−79.36%), respectively. Due to these biological activities, the WFNE3 nanoemulsion sample could be scaled up to the industrial level for the formulation of varied types of functional foods.

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Anti sickling potential and chemical profiling of traditionally used Woodfordia fruticosa (L.) Kurz leaves

Cited by 7 publications

References 44 publications

Exploring therapeutic potential of Woodfordia fruticosa (L.) Kurz leaf and bark focusing on antioxidant, antithrombotic, antimicrobial, anti‐inflammatory, analgesic, and antidiarrheal properties

Exploring therapeutic potential of Woodfordia fruticosa (L.) Kurz leaf and bark focusing on antioxidant, antithrombotic, antimicrobial, anti‐inflammatory, analgesic, and antidiarrheal properties

Antisickling and Antihemolytic Mechanism of Spirulina platensis (Oscillatoriaceae): A Nutraceutical Commonly Used in Cameroon

Woodfordia fruticosa extract nanoemulsion: Influence of processing treatment on droplet size and its assessment for in vitro antimicrobial and anti-inflammatory activity

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