Anti-Sca-1 antibody-functionalized vascular grafts improve vascular regeneration via selective capture of endogenous vascular stem/progenitor cells

He, Wei; Xing, Mengmeng; Deng, Weiliang; Qian, Meng; Wang, Fei; Wang, Kai; Midgley, Adam C.; Zhao, Qiang

doi:10.1016/j.bioactmat.2022.03.007

Cited by 11 publications

(10 citation statements)

References 74 publications

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“…Macrophages can be divided into activated macrophages (M1 phenotype) and alternatively activated macrophages (M2 phenotype). [ 45 ] The transformation of pro‐inflammatory M1 macrophages into reparative M2 macrophages is a key step in promoting wound healing, leading to the regression of inflammation and the promotion of tissue repair. CD86, the main synergistic factor that induces T lymphocyte proliferation and interleukin‐2 (IL‐2) production, is a characteristic marker of M1 macrophages.…”

Section: Resultsmentioning

confidence: 99%

Injectable Antibacterial Hydrogel with Asiaticoside‐Loaded Liposomes and Ultrafine Silver Nanosilver Particles Promotes Healing of Burn‐Infected Wounds

Feng

Liu²,

Chen

et al. 2023

Adv Healthcare Materials

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Post‐injury infection and wound healing are recurrent daily life problems. Therefore, the necessity of developing a biomaterial with antibacterial and wound‐healing properties is paramount. Based on the special porous structure of hydrogel, this work modifies recombinant collagen and quaternary ammonium chitosan and fused them with silver nanoparticles (Ag@mental‐organic framework (Ag@MOF)) with antibacterial properties, and asiaticoside‐loaded liposomes (Lip@AS) with anti‐inflammatory/vascularization effects to form the rColMA/QCSG/LIP@AS/Ag@MOF (RQLAg) hydrogel. The prepared hydrogel possesses good sustainable release capabilities of Ag+ and AS and exhibits concentration‐dependent swelling properties, pore size, and compressive strength. Cellular experiments show that the hydrogel exhibit good cell compatibility and promote cell migration, angiogenesis, and M1 macrophage polarization. Additionally, the hydrogels exhibit excellent antibacterial activity against Escherichia coli and Staphylococcus aureus in vitro. In vivo, Sprague Dawley rats burn‐wound infection model showed that the RQLAg hydrogel could efficiently promote wound healing and has stronger healing promoting abilities than those of Aquacel Ag. In summary, the RQLAg hydrogel is expected to be an excellent material for accelerating open wound healing and preventing bacterial infections.

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Section: Resultsmentioning

confidence: 99%

Injectable Antibacterial Hydrogel with Asiaticoside‐Loaded Liposomes and Ultrafine Silver Nanosilver Particles Promotes Healing of Burn‐Infected Wounds

Feng

Liu²,

Chen

et al. 2023

Adv Healthcare Materials

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“…The results suggested that the surface functionalization procedures employed in this study would not induce undesirable morphological changes. The peptide-modified scaffolds could also provide a favorable fibrous structure for cell growth and mechanical support …”

Section: Resultsmentioning

confidence: 99%

“…The peptide-modified scaffolds could also provide a favorable fibrous structure for cell growth and mechanical support. 35 Cell Adhesion and Proliferation on Peptide-Functionalized Electrospun Membranes. The cell adhesion, proliferation, and morphology of HUVECs cultured on various peptide-functionalized membranes with a similar grafting density of approximately 0.16 nmol/cm 2 were evaluated to study the cell−biomaterial interactions.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Surface Functionalization of Electrospun Scaffolds by QK-AG73 Peptide for Enhanced Interaction with Vascular Endothelial Cells

Bai,

Zhang,

Gao

et al. 2023

Langmuir

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Rapid endothelialization still remains challenging for blood-contacting biomaterials, especially for long-term, functional, small-diameter vascular grafts. The vascular endothelial growth factor (VEGF)-mimicking QK peptide holds great promise in promoting vascular endothelial cellular activities such as adhesion, spreading, proliferation, and migration. Syndecans are transmembrane proteoglycans that are highly expressed on cell surfaces, including vascular endothelial cells, which can act as docking receptors to provide binding sites for a variety of cellular growth and signaling molecules. Herein, a novel peptide QK-AG73 that coupled the QK domain with the syndecan binding peptide AG73 was proposed, aiming to synergistically enhance the interaction with vascular endothelial cells. In addition, mechanically matched bioactive scaffolds based on poly(L-lactide-co-εcaprolactone) were successfully prepared by surface functionalization of the covalently combined QK-AG73 peptide. The result showed that the adhesion of human umbilical vein endothelial cells (HUVECs) was increased by approximately 2-fold on QK-AG73-modified surface compared with those modified with a single QK or AG73 peptide. Moreover, surface functionalization of electrospun scaffolds by this QK-AG73 peptide was more efficient in specifically promoting the proliferation of HUVECs and allowing them to grow with an elongated cobblestone-like cell morphology. It was hypothesized that both VEGF receptors and transmembrane syndecan receptors were involved in cellular regulation by the QK-AG73 peptide, which resulted in synergistic improvement of the interactions with vascular endothelial cells and provided a promising strategy to promote endothelialization of small-diameter vascular grafts.

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“…Vascular injury, caused by blood flow shear stress, inflammation and fibrosis, is the pathological foundation and causes of multi-cardiovascular diseases. Meanwhile, vascular injury usually occurs after the surgery of arterial occlusive disease, such as carotid endarterectomy, balloon dilatation surgeries and so on [ 12 , 13 ]. The regeneration of injured vascular is a complex but orderly process.…”

Section: The Molecular Mechanism Of Vascular Regeneration After Injurymentioning

confidence: 99%

“…These systems have obvious advantages for systematic delivery of drugs in cardiovascular diseases, e.g. membrane encapsulating drugs at nanoscale can protect them from immune system and increase drug delivery efficiency with low toxicity [ 13 , 70 ]. Due to different cell-derived EVs playing distinct roles in the process of vascular regeneration, it is important to select suitable EV systems for the therapy of vascular injured diseases.…”

Section: The Different Derived Evs For Vascular Therapymentioning

confidence: 99%

Recent advances of natural and bioengineered extracellular vesicles and their application in vascular regeneration

Wang

Chen

et al. 2022

Regenerative Biomaterials

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The progression of cardiovascular diseases such as atherosclerosis and myocardial infarction leads to serious vascular injury, highlighting the urgent need for targeted regenerative therapy. Extracellular vesicles (EVs) composed of a lipid bilayer containing nuclear and cytosolic materials are relevant to the progression of cardiovascular diseases. Moreover, EVs from multiple cells will deliver bioactive cargo in pathological cardiovascular and regulate the biological function of recipient cells, such as inflammation, proliferation, angiogenesis, and polarization. However, because the targeting and bioactivity of natural EVs are subject to several limitations, bioengineered EVs have achieved wide advancements in biomedicine. Bioengineered EVs involve three main ways to acquire including 1) Modification of the EVs after isolation; 2) Modification of producer cells before EVs’ isolation; 3) Synthesize EVs using natural or modified cell membranes, and encapsulating drugs or bioactive molecules into EVs. In this review, we firstly summarize the cardiovascular injury-related disease and describe the role of different cells and EVs in vascular regeneration. We also discuss the application of bioengineered EVs from different producer cells to cardiovascular diseases. Finally, we summarize the molecular that can be modified in EVs to specifically target abnormal cells in injured vascular.

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Anti-Sca-1 antibody-functionalized vascular grafts improve vascular regeneration via selective capture of endogenous vascular stem/progenitor cells

Cited by 11 publications

References 74 publications

Injectable Antibacterial Hydrogel with Asiaticoside‐Loaded Liposomes and Ultrafine Silver Nanosilver Particles Promotes Healing of Burn‐Infected Wounds

Injectable Antibacterial Hydrogel with Asiaticoside‐Loaded Liposomes and Ultrafine Silver Nanosilver Particles Promotes Healing of Burn‐Infected Wounds

Surface Functionalization of Electrospun Scaffolds by QK-AG73 Peptide for Enhanced Interaction with Vascular Endothelial Cells

Recent advances of natural and bioengineered extracellular vesicles and their application in vascular regeneration

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