Anti-pesticide DNA aptamers fail to recognize their targets with asserted micromolar dissociation constants

Zara, Lorena; Achilli, Silvia; Chovelon, Benoît; Fiore, Emmanuelle; Toulmé, Jean‐Jacques; Peyrin, Eric; Ravelet, Corinne

doi:10.1016/j.aca.2021.338382

Cited by 14 publications

(14 citation statements)

References 56 publications

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“…Recently, a number of papers have been published to scrutinize the binding of some commonly used aptamers 52 for important analytes such as arsenic, 53 ethanolamine, 54 ampicillin, 55 and some pesticides. 56 None of them showed binding. In this work, another example was discovered.…”

Section: Sensors and Diagnostics Papermentioning

confidence: 96%

Comparing two cortisol aptamers for label-free fluorescent and colorimetric biosensors

et al. 2022

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Section: Sensors and Diagnostics Papermentioning

confidence: 96%

Comparing two cortisol aptamers for label-free fluorescent and colorimetric biosensors

et al. 2022

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“…Recently, quite a few papers have been published to scrutinize the aptamers for a few small molecules such as arsenic, ampicillin, chloramphenicol and a few pesticides, [30][31][32][33][34][35] where careful homogeneous binding assays revealed no binding. 36 Given that heterogeneous aptamer binding assays are more difficult to interpret, 37,38 and intrigued by the reported binding assays for ethanolamine, in this work, we examined the binding of the ethanolamine aptamers using homogeneous binding assays.…”

Section: Introductionmentioning

confidence: 99%

Homogeneous assays for aptamer-based ethanolamine sensing: no indication of target binding

Ding

Liu

Huang

et al. 2022

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“…Over the last decades, biosensor technology has been proving its capability to complement or replace many of the complicated, relatively expensive, and time-consuming traditional analytical methods [ 1 , 2 , 3 , 4 ]. More recently, the use of nucleic acid aptamers as biorecognition elements has brought numerous advantages in comparison with the enzymes or antibodies commonly used in biosensors, such as smaller size, better stability, reversible binding ability, simple chemical modification, and a lower cost [ 4 , 5 , 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

Critical Design Factors for Electrochemical Aptasensors Based on Target-Induced Conformational Changes: The Case of Small-Molecule Targets

et al. 2022

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Nucleic-acid aptamers consisting in single-stranded DNA oligonucleotides emerged as very promising biorecognition elements for electrochemical biosensors applied in various fields such as medicine, environmental, and food safety. Despite their outstanding features, such as high-binding affinity for a broad range of targets, high stability, low cost and ease of modification, numerous challenges had to be overcome from the aptamer selection process on the design of functioning biosensing devices. Moreover, in the case of small molecules such as metabolites, toxins, drugs, etc., obtaining efficient binding aptamer sequences proved a challenging task given their small molecular surface and limited interactions between their functional groups and aptamer sequences. Thus, establishing consistent evaluation standards for aptamer affinity is crucial for the success of these aptamers in biosensing applications. In this context, this article will give an overview on the thermodynamic and structural aspects of the aptamer-target interaction, its specificity and selectivity, and will also highlight the current methods employed for determining the aptamer-binding affinity and the structural characterization of the aptamer-target complex. The critical aspects regarding the generation of aptamer-modified electrodes suitable for electrochemical sensing, such as appropriate bioreceptor immobilization strategy and experimental conditions which facilitate a convenient anchoring and stability of the aptamer, are also discussed. The review also summarizes some effective small molecule aptasensing platforms from the recent literature.

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Anti-pesticide DNA aptamers fail to recognize their targets with asserted micromolar dissociation constants

Cited by 14 publications

References 56 publications

Comparing two cortisol aptamers for label-free fluorescent and colorimetric biosensors

Comparing two cortisol aptamers for label-free fluorescent and colorimetric biosensors

Homogeneous assays for aptamer-based ethanolamine sensing: no indication of target binding

Critical Design Factors for Electrochemical Aptasensors Based on Target-Induced Conformational Changes: The Case of Small-Molecule Targets

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