Anti-PcrV IgY antibodies protect against Pseudomonas aeruginosa infection in both acute pneumonia and burn wound models

Ranjbar, Mahya; Behrouz, Bahador; Norouzi, Fatemeh; Gargari, Seyed Latif Mousavi

doi:10.1016/j.molimm.2019.10.005

Cited by 33 publications

(26 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Ranjbar et al have recently shown that anti-PcrV IgY was more protective against P. aeruginosa acute pneumonia and burn-associated infections than control IgY. IgY was comparable to IgG and could serve to be a more affordable alternative in the future [93].…”

Section: Needle Tipmentioning

confidence: 99%

Antibodies Inhibiting the Type III Secretion System of Gram-Negative Pathogenic Bacteria

Hotinger

May

2020

Antibodies

View full text Add to dashboard Cite

Pathogenic bacteria are a global health threat, with over 2 million infections caused by Gram-negative bacteria every year in the United States. This problem is exacerbated by the increase in resistance to common antibiotics that are routinely used to treat these infections, creating an urgent need for innovative ways to treat and prevent virulence caused by these pathogens. Many Gram-negative pathogenic bacteria use a type III secretion system (T3SS) to inject toxins and other effector proteins directly into host cells. The T3SS has become a popular anti-virulence target because it is required for pathogenesis and knockouts have attenuated virulence. It is also not required for survival, which should result in less selective pressure for resistance formation against T3SS inhibitors. In this review, we will highlight selected examples of direct antibody immunizations and the use of antibodies in immunotherapy treatments that target the bacterial T3SS. These examples include antibodies targeting the T3SS of Pseudomonas aeruginosa, Yersinia pestis, Escherichia coli, Salmonella enterica, Shigella spp., and Chlamydia trachomatis.

show abstract

Section: Needle Tipmentioning

confidence: 99%

Antibodies Inhibiting the Type III Secretion System of Gram-Negative Pathogenic Bacteria

Hotinger

May

2020

Antibodies

View full text Add to dashboard Cite

show abstract

“…Titers. Antigen-specific IgY titers against whole-cell P. aeruginosa PAO1 as well as r-OprF were assessed by ELISA, as described previously [25]. Briefly, each ELISA plate well (Nunc, USA) was coated with 10 8 CFU of P. aeruginosa PAO1 or 2.5 μg r-OprF in 15 mM Na 2 CO 3 and 35 mM NaHCO 3 (pH 9.6), incubated overnight at 4°C, washed with 0.05% T-PBS, and blocked with PBS +5% skim milk.…”

Section: Evaluation Of Anti-oprf Igymentioning

confidence: 99%

“…To test the inhibitory activity of anti-OprF IgY antibodies on P. aeruginosa invasion to the A549 cell line, a gentamicin protection assay was performed, and we followed the methods of Ranjbar et al [25]. Briefly, anti-OprF IgY antibodies were mixed with PAO1 strain and then added to confluent A549 cells seeded in a 24-well plate (Nunc).…”

Section: Invasion Assaymentioning

confidence: 99%

“…The burn control group comprised untreated mice with burn wounds that were not infected. The survival rate of experimental mice was monitored twice daily up to 5 days, which were analyzed using the Mantel-Cox log-rank test [25].…”

Section: Invasion Assaymentioning

confidence: 99%

“…The data were analyzed by one-way analysis of variance with Tukey's multiple comparison tests. Survival analysis for different mouse groups was performed using the Kaplan Meier survival curve with the Mantel-Cox log-rank test [25]. All results were expressed as the mean ± standard deviation (SD).…”

Section: Invasion Assaymentioning

confidence: 99%

See 2 more Smart Citations

Immunotherapy with IgY Antibodies toward Outer Membrane Protein F Protects Burned Mice against Pseudomonas aeruginosa Infection

Norouzi

Behrouz

Ranjbar

et al. 2020

Journal of Immunology Research

Self Cite

View full text Add to dashboard Cite

Burn patients with multidrug-resistant Pseudomonas aeruginosa infections commonly suffer from high morbidity and mortality, which present a major challenge to healthcare systems throughout the world. Outer membrane protein F (OprF), as a main outer membrane porin, is required for full virulence expression of P. aeruginosa. The aim of this study was to evaluate the protective efficacy of egg yolk-specific antibody (IgY) raised against recombinant OprF (r-OprF) protein in a murine burn model of infection. The hens were immunized with r-OprF, and anti-r-OprF IgY was purified using salt precipitation. Groups of mice were injected with different regimens of anti-OprF IgY or control IgY (C-IgY). Infections were caused by subcutaneous injection of P. aeruginosa strain PAO1 at the burn site. Mice were monitored for mortality for 5 days. The functional activity of anti-OprF IgY was determined by in vitro invasion assays. Immunotherapy with anti-OprF IgY resulted in a significant improvement in the survival of mice infected by P. aeruginosa from 25% to 87.5% compared with the C-IgY and PBS. The anti-OprF IgY decreased the invasion of P. aeruginosa PAO1 into the A549. Passive immunization with anti-OprF IgY led to an efficacious protection against P. aeruginosa burn infection in the burn model.

show abstract

Pseudomonas aeruginosa Antivirulence Strategies: Targeting the Type III Secretion System

Goldberg

Crisan

Luu

2022

Advances in Experimental Medicine and Biology

View full text Add to dashboard Cite

Anti-PcrV IgY antibodies protect against Pseudomonas aeruginosa infection in both acute pneumonia and burn wound models

Cited by 33 publications

References 65 publications

Antibodies Inhibiting the Type III Secretion System of Gram-Negative Pathogenic Bacteria

Antibodies Inhibiting the Type III Secretion System of Gram-Negative Pathogenic Bacteria

Immunotherapy with IgY Antibodies toward Outer Membrane Protein F Protects Burned Mice against Pseudomonas aeruginosa Infection

Pseudomonas aeruginosa Antivirulence Strategies: Targeting the Type III Secretion System

Contact Info

Product

Resources

About