Anti-Osteoclastogenic Activity of Praeruptorin A via Inhibition of p38/Akt-c-Fos-NFATc1 Signaling and PLCγ-Independent Ca2+ Oscillation

Yeon, Jeong-Tae; Kim, Kwang‐Jin; Choi, Sik‐Won; Moon, Sang‐Jin; Park, Young Sik; Ryu, Beyong Hwan; Oh, Jae‐Min; Kim, Min Seuk; Erkhembaatar, Munkhsoyol; Son, Young–Jin; Kim, Seong Hwan

doi:10.1371/journal.pone.0088974

Cited by 27 publications

(26 citation statements)

References 44 publications

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“…Phytochemical and pharmacological studies showed that angular-type pyranocoumarins are the major constituents of this plant [3][4][5][6][7][8][9], and these compounds have various beneficial effects such as anti-inflammatory [10][11][12], antiasthma [13], chemopreventive [14], smooth muscle relaxant [15], neuroprotective [16], and anti-osteoclastogenic properties [17].…”

Section: Introductionmentioning

confidence: 99%

Pyranocoumarins from Root Extracts of Peucedanum praeruptorum Dunn with Multidrug Resistance Reversal and Anti-Inflammatory Activities

Lee

Kim

et al. 2015

Molecules

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Abstract:In the search for novel herbal-based anticancer agents, we isolated a new angular-type pyranocoumarin, (+)-cis-(3 1 S,4 1 S)-3 1 -angeloyl-4 1 -tigloylkhellactone (1) along with 12 pyranocoumarins (2-13), two furanocoumarins (14, 15), and a polyacetylene (16) were isolated from the roots of Peucedanum praeruptorum using chromatographic separation methods. The structures of the compounds were determined using spectroscopic analysis with nuclear magnetic resonance (NMR) and high-resolution-electrospray ionization-mass spectrometry (HR-ESI-MS). The multidrug-resistance (MDR) reversal and anti-inflammatory effects of all the isolated compounds were evaluated in human sarcoma MES-SA/Dx5 and lipopolysaccharide (LPS)-induced RAW 264.7 cells. Among the 16 tested compounds, two (2 and 16) downregulated nitric oxide (NO) production and five (1, 7, 8, 11, and 13) inhibited the efflux of drugs by MDR protein, indicating the reversal of MDR. Therefore, these compounds may be potential candidates for the development of effective agents against MDR forms of cancer.

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Section: Introductionmentioning

confidence: 99%

Pyranocoumarins from Root Extracts of Peucedanum praeruptorum Dunn with Multidrug Resistance Reversal and Anti-Inflammatory Activities

Lee

Kim

et al. 2015

Molecules

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“…Here, IL‐7 decreased the phosphorylation of p38 and Akt without affecting JNK and ERK. It was demonstrated that the p38 signaling pathway has a direct involvement in mediating the induction of c‐Fos and NFATc1 during RANKL‐induced osteoclastogenesis (Yeon et al, ). A recent study showed that the Akt‐NFATc1 signaling axis has been known to play a critical role in osteoclast differentiation (Moriwaki et al, ), therefore the osteoclastogenic action of IL‐7/IL7R could activate the c‐Fos/c‐Jun pathway and up‐regulate NFATc1expression via the p38 and Akt signaling pathways.…”

Section: Discussionmentioning

confidence: 99%

Effects of interleukin‐7/interleukin‐7 receptor on RANKL‐mediated osteoclast differentiation and ovariectomy‐induced bone loss by regulating c‐Fos/c‐Jun pathway

Zhao

Zhaofeng

et al. 2018

Journal Cellular Physiology

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To explore the effects of IL-7/IL-7R on the RANKL-mediated osteoclast differentiation in vitro and OVX-induced bone loss in vivo. BMMs and RAW264.7 were transfected with IL-7, IL-7R siRNA, c-Fos siRNA, and c-jun siRNA and later stimulated by RANKL. TRAP and toluidine blue staining were used to observe osteoclast formation and bone resorption, respectively. HE and TRAP staining were used to detect trabecular bone microstructure and osteoclasts of mice, respectively. qRT-PCR and Western blot analysis were used to examine expression. IL-7 unregulated the expression of CTSK, NFATc1, MMP9, and the phosphorylation of p38 and Akt by activating the c-Fos/c-Jun pathway, which increased osteoclast numbers and bone resorption in RANKL-stimulated macrophages. While IL-7R siRNA and c-Fos siRNA decreased the expression, as well as and the phosphorylation of p38 and Akt.IL-7 decreased the BMD and OPG expression in OVX-induced mice and increased the TRAP positive cells, the mRNA expression of c-fos, c-jun, and RANKL, which was contradictory to IL-7R siRNA, and c-Fos siRNA. Furthermore, IL-7R siRNA and c-Fos siRNA caused thicker trabeculae, increased trabecular number, and decreased osteolysis in OVX mice. IL-7/IL-7R can promote RANKL-mediated osteoclast formation and bone resorption by activating the c-Fos/c-Jun pathway, as well as inducing bone loss in OVX mice.

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“…Western blot and real-time PCR analysis were performed as described previously. 37 Primers for real-time PCR were chosen with the online Primer3 design program. 38 The primer sets used in this study are shown in Table 2.…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

Acredinone C and the Effect of Acredinones on Osteoclastogenic and Osteoblastogenic Activity

Yeon¹,

Kim²,

Lee³

et al. 2016

J. Nat. Prod.

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A new inhibitor, acredinone C (1), of receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclast differentiation was isolated from the culture broth of the fungus Acremonium sp. (F9A015) along with acredinones A (2) and B (3). The structure of acredinone C (1), which incorporates benzophenone and xanthone moieties, was established by the analyses of combined spectroscopic data including 1D and 2D NMR and MS. All of the acredinones studied efficiently inhibited the RANKL-induced formation of TRAP(+)-MNCs in a dose-dependent manner without any cytotoxicity up to 10 μM. Acredinone A showed dual activity in both osteoclast and osteoblast differentiation in vitro and good efficacy in an animal disease model of bone formation.

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Anti-Osteoclastogenic Activity of Praeruptorin A via Inhibition of p38/Akt-c-Fos-NFATc1 Signaling and PLCγ-Independent Ca2+ Oscillation

Cited by 27 publications

References 44 publications

Pyranocoumarins from Root Extracts of Peucedanum praeruptorum Dunn with Multidrug Resistance Reversal and Anti-Inflammatory Activities

Pyranocoumarins from Root Extracts of Peucedanum praeruptorum Dunn with Multidrug Resistance Reversal and Anti-Inflammatory Activities

Effects of interleukin‐7/interleukin‐7 receptor on RANKL‐mediated osteoclast differentiation and ovariectomy‐induced bone loss by regulating c‐Fos/c‐Jun pathway

Acredinone C and the Effect of Acredinones on Osteoclastogenic and Osteoblastogenic Activity

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