Anti-Leishmania amazonensis activity of the terpenoid fraction from Eugenia pruniformis leaves

Albuquerque, Ricardo Diego Duarte Galhardo de; Oliveira, Adriana Passos; Ferreira, Christian; Passos, Carlos Luan Alves; Fialho, Eliane; Soares, Deivid Costa; Amaral, Verônica F.; Bezerra, Gabrielle B; Esteves, Rui; Santos, Marcelo Guerra; Albert, André Luís Mazzei; Rocha, Leandro

doi:10.1590/0001-3765202020201181

Cited by 9 publications

(3 citation statements)

References 27 publications

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“…The Myrtaceae family has great diversity and is found mainly in tropical and temperate areas of the globe (Judd et al, 2009). Ethnobotanical studies conducted on several family species indicate numerous popular uses, such as treating diarrhea, sore throat, gout, rheumatism, flu, urinary tract diseases, and diabetes (Boscolo and Senna-Valle, 2008) The genus Eugenia, belonging to the Myrtaceae family, the most commonly used folk medicine in Restinga Jurubatiba National Park in Carapebus city, RJ, Brazil (28) Regarding the constituents and biological activities of E. pruniformis, these remain scarce, and only the essential oil and antileishmanial activity of the terpenoids of this species have been previously reported (Albuquerque et al, 2012(Albuquerque et al, , 2020 For the species E. sulcata, there is only one study on its insecticidal and acaricide potential; however, according to the authors, other chemical and physiological studies are needed to determine whether these substances can be helpful in the control of arthropods and vector transmission in the field (Feder et al, 2019). Therefore, we evaluated the metabolic activity and cell death by necrosis in these two species in the present study.…”

Section: Discussionmentioning

confidence: 99%

Comparative evaluation of plant extract effects on peritoneal, medullary and J774 cells. G8 macrophages

et al. 2023

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The use of medicinal plants as raw material for extracts production and pure substances isolation and subsequence development of new drugs represents a constantly growing area. However, some stages are indispensable before pharmacologically evaluating natural products such as medicines. Toxicity tests in mammalian cells are essential to initiate new drugs development or verify the substance’s biocompatibility. Thus, we verified the toxicity of crude extracts and fractions with different polarities obtained from the leaves and stems of eight plant species. The toxic effect was evaluated on macrophages obtained from the bone marrow and peritoneal cavity of a Swiss webster mouse and J774 macrophages. G8 cell lineage. These macrophages were cultured in a 96-well plate, and the compounds were added at a concentration of 100 µg/mL for 24 hours. After this time, the supernatant was removed. The toxicity was evaluated for lactate dehydrogenase (LDH) release assay and the resazurin assay, which uses an indicator dye to measure oxidation-reduction reactions. The results showed a difference in the percentage of toxicity when comparing the same extract in different types of macrophages. This outcome indicates that these cells from different origins may exhibit different responses when exposed to the same natural compounds.

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Section: Discussionmentioning

confidence: 99%

Comparative evaluation of plant extract effects on peritoneal, medullary and J774 cells. G8 macrophages

et al. 2023

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“…Additionally, it has been observed that apoptotic processes induced by oxidative stress are favored in cells treated with ursolic, betulinic and pomolic acids (Liu et al, 2014;Lewinska et al, 2017;Zheng et al, 2019;Wang et al, 2021). Other studies have found inhibitory effects in trypanosomatid species after exposure to betulinic and ursolic acids associated with alterations in the mitochondrial membrane potential and increases in oxidative stress, which in turn implies effects on oxidative phosphorylation (Yamamoto et al, 2015;Bossolani et al, 2017;Sousa et al, 2017;Albuquerque et al, 2020). With this panorama, two possible hypotheses arise as an explanation for glucose deprivation or depletion.…”

Section: Frontiers In Molecular Biosciencesmentioning

confidence: 99%

Clethra fimbriata hexanic extract triggers alteration in the energy metabolism in epimastigotes of Trypanosoma cruzi

Pardo-Rodriguez,

Lasso,

Santamaría-Torres

et al. 2023

Front. Mol. Biosci.

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Chagas disease (ChD), caused by Trypanosoma cruzi, is endemic in American countries and an estimated 8 million people worldwide are chronically infected. Currently, only two drugs are available for therapeutic use against T. cruzi and their use is controversial due to several disadvantages associated with side effects and low compliance with treatment. Therefore, there is a need to search for new tripanocidal agents. Natural products have been considered a potential innovative source of effective and selective agents for drug development to treat T. cruzi infection. Recently, our research group showed that hexanic extract from Clethra fimbriata (CFHEX) exhibits anti-parasitic activity against all stages of T. cruzi parasite, being apoptosis the main cell death mechanism in both epimastigotes and trypomastigotes stages. With the aim of deepening the understanding of the mechanisms of death induced by CFHEX, the metabolic alterations elicited after treatment using a multiplatform metabolomics analysis (RP/HILIC-LC-QTOF-MS and GC-QTOF-MS) were performed. A total of 154 altered compounds were found significant in the treated parasites corresponding to amino acids (Arginine, threonine, cysteine, methionine, glycine, valine, proline, isoleucine, alanine, leucine, glutamic acid, and serine), fatty acids (stearic acid), glycerophospholipids (phosphatidylcholine, phosphatidylethanolamine and phosphatidylserine), sulfur compounds (trypanothione) and carboxylic acids (pyruvate and phosphoenolpyruvate). The most affected metabolic pathways were mainly related to energy metabolism, which was found to be decrease during the evaluated treatment time. Further, exogenous compounds of the triterpene type (betulinic, ursolic and pomolic acid) previously described in C. fimbriata were found inside the treated parasites. Our findings suggest that triterpene-type compounds may contribute to the activity of CFHEX by altering essential processes in the parasite.

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“…Diosgenin, βsitosterol, furostanol saponinscostusosides, βDglucoside, prosapogenins, dioscin, gracillin, dihydrophytylplastoquinone, and αtocopherolquinone [4]. Among them; Flavonoids, alkaloids, tannins, and polyphenols exert antiviral properties, anti-inflammatory properties, anti-bacterial, antiproliferative, and wound healing properties [6][7][8].…”

Section: Introductionmentioning

confidence: 99%

Costus speciosus leaf and seed extracts for wound healing: a comparative evaluation using mice excision wound models

Zishan,

Uddin,

Mohammad

et al. 2024

Clin Phytosci

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Background The present study investigated the potential wound healing activity comparisons between ethanol leaf & seed extracts of Costus speciosus topical formulation using mice Excision wound models, compared to Neomycin sulphate ointment as a standard drug. To assess the efficacy of Costus speciosus ointment as a potential alternative to commercially available ointments, a study was conducted using a cohort of 16 healthy male mice, aged between 3 and 4 months. The ointment formulation was prepared utilizing ethanol extracts from both the leaves and seeds, presented in a dosage form for application. This investigation seeks to provide insights into the comparative effectiveness of Costus speciosus ointments in relation to conventional market preparations, with a focus on its potential applicability as an alternative therapeutic option. The parameter measured was wound contraction and epithelization period. Result The mice groups treated with seed & leave extracts ointment showed better wound size changes than the negative and standard groups. However, the leaf extract ointment promotes the formation of granulation in tissue, allowing the reepithelization phase to occur more rapidly than seed extract. From the 1st day to the results of the 9th day of the extract indicate that there is a significant increase (P < 0.05) in the percentage of wound contraction in the group. After 21 days the wound was healed fully. These results suggest that ointment of ethanol leaf extract of Costus speciosus could be an answer to facilitate wound healing compared to seed extract, to approve the traditional claims of the plant in wound healing activity. Conclusion The study concludes that ethanol leaf and seed extracts of Costus speciosus exhibit potential wound healing activity in mice excision wound models. The topical formulation of these extracts significantly enhances wound closure, reduces wound size, and increases tensile strength compared to the control group. The efficacy of the extracts is comparable to the standard drug, Neomycin sulphate ointment. Therefore, the use of Costus speciosus extracts in topical formulations can be considered a promising alternative for the treatment of wounds. However, further studies are needed to determine the safety and efficacy of these extracts in humans.

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Anti-Leishmania amazonensis activity of the terpenoid fraction from Eugenia pruniformis leaves

Cited by 9 publications

References 27 publications

Comparative evaluation of plant extract effects on peritoneal, medullary and J774 cells. G8 macrophages

Comparative evaluation of plant extract effects on peritoneal, medullary and J774 cells. G8 macrophages

Clethra fimbriata hexanic extract triggers alteration in the energy metabolism in epimastigotes of Trypanosoma cruzi

Costus speciosus leaf and seed extracts for wound healing: a comparative evaluation using mice excision wound models

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