Anti-Inflammatory Effects ofLiriope platyphyllain LPS-Stimulated Macrophages and Endotoxemic Mice

Abstract

In the present study, the anti-inflammatory and antisepticemic activities of a water extract of Liriope platyphylla (LP) were investigated. We first estimated the scavenging activity of DPPH and the hydroxyl radical and total phenolic contents of LP. Results indicated that LP, a rich source of phenolic compounds, showed a remarkable radical scavenging capacity. A MTT assay showed that LP treatment did not affect the toxicity against the RAW 264.7 macrophage cells, up to the concentration of 500[Formula: see text][Formula: see text]g/mL. Treatment of LP significantly attenuated the production of inflammatory mediators, such as nitric oxide (NO), interleukin-6 (IL-6), tumor-necrosis factor (TNF)-[Formula: see text] and prostaglandin (PG)E in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages cells. Moreover, LP contributed to the down-regulation of inducible NO synthase (iNOS) and TNF-[Formula: see text] mRNA expression, as well as cyclooxygenase-2 (COX-2) protein expression. A western blotting assay further showed that LP inhibited activation of mitogen-activated protein kinase (MAPK) and nuclear factor (NF)-[Formula: see text]B. In an animal experiment using an LPS-induced septicemia model in C57BL/6 mice, oral administration of LP (40[Formula: see text]mg/kg body weight) markedly reduced the level of TNF-[Formula: see text] and IL-6 in serum and protected against LPS-induced lethal shock in mice. Taken together, the results of treatments of LP on inhibited LPS-induced inflammatory responses in both in vitro and in vivo models and indicate it may be a promising neutraceutical or medicinal agent to prevent or cure inflammation-related disease.

Anti-inflammatory potential of Petiveria alliacea on activated RAW264.7 murine macrophages

“…Cells were cultured in DMEM supplemented with 10% of FBS, streptomycin (100 μg/ml), and penicillin (100 units/ml) incubated overnight at 37°C and 5% CO 2 . [ 12 ]…”

Biological Mechanisms and Related Natural Inhibitors of CD36 in Nonalcoholic Fatty Liver

“…Liriope platyphylla is traditional medicine, which suggested a protective role of LPE in anti-obesity and anti-inflammatory effects. 111 Le TNH et al 112 showed that the treatment with Liriope platyphylla root ethanolic extract (LPE) reduced the protein expression of CD36 both in vivo and in vitro, leading to reduced fatty acid uptake.…”

Antiencephalitic Activities of Bioactive Substances Partially Purified From Liriope Platyphylla Tuber.

“…The hot water extract from L. platyphylla tuber inhibits the production of inflammatory mediators such as nitric oxide (NO), interleukin-6 (IL-6), tumor necrosis factor (TNF) and prostaglandin (PG) E2 on RAW 264.7 macrophages cells treated by LPS (Kim et al, 2016). Therefore, in this study, antiencephalitic activity was measured for centrifugal supernatant and pellet according to methanol concentration to analyze functional materials in hot water and homogenized extracts.…”