Anti-inflammatory and Antioxidative Effects of Tetrahedral DNA Nanostructures via the Modulation of Macrophage Responses

Abstract

Tetrahedral DNA nanostructures (TDNs) are a new type of nanomaterials that have recently attracted attention in the field of biomedicine. However, the practical application of nanomaterials is often limited owing to the host immune response. Here, the response of RAW264.7 macrophages to TDNs was comprehensively evaluated. The results showed that TDNs had no observable cytotoxicity and could induce polarization of RAW264.7 cells to the M1 type. TDNs attenuated the expression of NO IL-1β (interleukin-1β), IL-6 (interleukin-6), and TNF-α (tumor necrosis factor-α) in LPS-induced RAW264.7 cells by inhibiting MAPK phosphorylation. In addition, TDNs inhibited LPS-induced reactive oxygen species (ROS) production and cell apoptosis by up-regulating the mRNA expression of antioxidative enzyme heme oxygenase-1 (HO-1). The findings of this study demonstrated that TDNs have great potential as a novel theranostic agent because of their anti-inflammatory and antioxidant activities, high bioavailability, and ease of targeting.

Tetrahedral Framework Nucleic Acids Promote Corneal Epithelial Wound Healing in Vitro and in Vivo

“…According to our results of the experiment in vitro, HCECs proliferated and migrated faster to repair the injured area after exposure to tFNAs. Our previous experiments showed that tFNAs can inhibit cell apoptosis as well as inflammatory response in cells . In conclusion, the promotion effect of tFNAs on corneal epithelial wound healing could be explained as the anti‐inflammatory, anti‐oxidant, and regenerative potentials of tFNAs.…”

Effects of tetrahedral framework nucleic acid/wogonin complexes on osteoarthritis

“…liferation and promoting the migration of chondrocytes, TFNA, as a novel DNA material, can exhibit anti-inflammation and antioxidation activity in macrophages by suppressing the phosphorylation of members of the MAPK subfamilies. 21,22,41 Therefore, in this study, we aimed to explore how the combination of TFNA with wogonin affected inflammatory chondrocytes induced by IL-1β and inflamed knee joints in rats.…”

Multi-targeted Antisense Oligonucleotide Delivery by a Framework Nucleic Acid for Inhibiting Biofilm Formation and Virulence

“…In particular, FNAs are highly editable and can function as carriers for nucleic acid drugs to enter cells [18][19][20]. Indeed, various such drugs, including aptamers or cytosine-phosphate-guanine, have been designed for FNAs to provide anticancer or immunostimulatory activities [21][22][23].…”